Background:The immune system of insects exerts fundamentally different antiviral mechanisms than mammals.MicroRNAs(miRNAs)play vital roles in developing insect antiviral immunity.MiRNAs expression profiles of insects ...Background:The immune system of insects exerts fundamentally different antiviral mechanisms than mammals.MicroRNAs(miRNAs)play vital roles in developing insect antiviral immunity.MiRNAs expression profiles of insects changed significantly during baculovirus infection.Methods:Differential expression profiles of miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing(NGS)and RT-qPCR during Autographa californica multiple nucleopolyhedrovirus(AcMNPV)infection.The transcription levels of genes were detected by RT-qPCR.The 50%tissue culture infective dose(TCID_(50))endpoint dilution assay was used to determine the proliferation of progeny virus.Results:NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,and 10 of them were significantly up-or down-regulated.Though RT-qPCR analysis,we observed the similar trends for the expression patterns of significantly differentially expressed miRNAs from NGS.Moreover,the transcription levels of core genes,Exportin5,Dicer1,and Argonaute1,in miRNA biogenesis pathways were significantly increased after AcMNPV infection.For five selected miRNAs,miR-34-5p could regulate the proliferation of baculovirus progeny virus and energy metabolism.Conclusion:The miRNAs biogenesis pathway in Sf9 cells plays an important role and may be stimulated to resist AcMNPV infection.This work provides evidence for the molecular mechanism of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology.展开更多
The Autographa californica multiple capsid nucleopolyhedrovirus (AcMNPV) was the first baculovirus for which the complete nucleotide sequence became known. Since then 15 years lapsed and much research has been perform...The Autographa californica multiple capsid nucleopolyhedrovirus (AcMNPV) was the first baculovirus for which the complete nucleotide sequence became known. Since then 15 years lapsed and much research has been performed to elucidate putative functions of the annotated open reading frames of this virus and this endeavour is still ongoing. AcMNPV is the most well-known and well-studied baculovirus species, not in the least for its application as a vector for the high-level expression of foreign genes in insect cells. This article is the first monograph of a single baculovirus and gives a current overview of what is known about the 151 AcMNPV ORFs, including (putative) function and temporal and spatial presence of transcripts and protein. To date 60 ORFs have a proven function, another 19 ORFs have homologs for which functions are known in other baculoviruses and 72 ORFs are still enigmatic. This paper should assist the reader in quickly finding the essentials of AcMNPV.展开更多
分别研究了甜菜夜蛾在幼虫期和成虫期对苜蓿银纹夜蛾核多角体病毒(AcNPV)的敏感性.结果表明,AcNPV对4龄甜菜夜蛾幼虫的致死中浓度为1 ml 9.1×106 PIB(多角体),4龄幼虫摄入亚致死剂量病毒对化蛹产生不良影响,并使性比发生变化,雌雄...分别研究了甜菜夜蛾在幼虫期和成虫期对苜蓿银纹夜蛾核多角体病毒(AcNPV)的敏感性.结果表明,AcNPV对4龄甜菜夜蛾幼虫的致死中浓度为1 ml 9.1×106 PIB(多角体),4龄幼虫摄入亚致死剂量病毒对化蛹产生不良影响,并使性比发生变化,雌雄蛹比为0.6～0.8,较对照明显升高;幼虫期摄入病毒亦对其成虫的繁殖力产生影响,无论是每雌产卵量、总卵量还是卵孵化率均比对照显著降低,交配比例和次数也比对照明显下降.甜菜夜蛾成虫期摄入病毒亦对繁殖力产生影响,产卵量和交配比例和次数以及卵孵率均比对照明显下降,此外,还对后代幼虫的存活率有显著影响.展开更多
The arrangement patterns of nucleocapsids within the envelope of \%Autographa californica\% nuclear polyhedrosis virus were studied by electron microscopy. Numbers of nucleocapsids observed in an envelope in their cro...The arrangement patterns of nucleocapsids within the envelope of \%Autographa californica\% nuclear polyhedrosis virus were studied by electron microscopy. Numbers of nucleocapsids observed in an envelope in their cross sections ranged from 1 to 17, and the frequencies at 2,3,4,5,6 and 7 nucleocapsids were significantly higher than the others, suggesting that these numbers of nucleocapsids were more commonly involved in an envelope. The regular arrangement patterns of nucleocapsids within envelope were observed in cross sections. Envelope outlines can be classified into a triangle (3 nucleocapsids in an envelope),lozenge and square (4 nucleocapsids in an envelope), trapeziun (5 nucleocapsids in an envelope), pentagons (5 or 8 nucleocapsids in an envelope) and hexagons (7 or 10 nucleocapsids in an envelope). The irregular arrangement patterns of nucleocapsids within envelopes were also observed in cross sections.展开更多
基金funded by the Natural Science Foundation of Shanxi Province,Grant No.201801D121193the Central Government Guiding Local Science and Technology Development Fund Project,Grant No.YDZJSX2022A001.
文摘Background:The immune system of insects exerts fundamentally different antiviral mechanisms than mammals.MicroRNAs(miRNAs)play vital roles in developing insect antiviral immunity.MiRNAs expression profiles of insects changed significantly during baculovirus infection.Methods:Differential expression profiles of miRNAs in Spodoptera frugiperda were monitored by next-generation sequencing(NGS)and RT-qPCR during Autographa californica multiple nucleopolyhedrovirus(AcMNPV)infection.The transcription levels of genes were detected by RT-qPCR.The 50%tissue culture infective dose(TCID_(50))endpoint dilution assay was used to determine the proliferation of progeny virus.Results:NGS revealed that 49 miRNAs were differentially expressed in Sf9 cells,and 10 of them were significantly up-or down-regulated.Though RT-qPCR analysis,we observed the similar trends for the expression patterns of significantly differentially expressed miRNAs from NGS.Moreover,the transcription levels of core genes,Exportin5,Dicer1,and Argonaute1,in miRNA biogenesis pathways were significantly increased after AcMNPV infection.For five selected miRNAs,miR-34-5p could regulate the proliferation of baculovirus progeny virus and energy metabolism.Conclusion:The miRNAs biogenesis pathway in Sf9 cells plays an important role and may be stimulated to resist AcMNPV infection.This work provides evidence for the molecular mechanism of baculovirus-insect interaction and offers novel ideas and directions for green pest control technology.
基金The project BACULOGENES of the European Union (LSHB-CT-2006-037541)The Netherlands Scientific Organisation (NWO) MEERVOUD program
文摘The Autographa californica multiple capsid nucleopolyhedrovirus (AcMNPV) was the first baculovirus for which the complete nucleotide sequence became known. Since then 15 years lapsed and much research has been performed to elucidate putative functions of the annotated open reading frames of this virus and this endeavour is still ongoing. AcMNPV is the most well-known and well-studied baculovirus species, not in the least for its application as a vector for the high-level expression of foreign genes in insect cells. This article is the first monograph of a single baculovirus and gives a current overview of what is known about the 151 AcMNPV ORFs, including (putative) function and temporal and spatial presence of transcripts and protein. To date 60 ORFs have a proven function, another 19 ORFs have homologs for which functions are known in other baculoviruses and 72 ORFs are still enigmatic. This paper should assist the reader in quickly finding the essentials of AcMNPV.
文摘分别研究了甜菜夜蛾在幼虫期和成虫期对苜蓿银纹夜蛾核多角体病毒(AcNPV)的敏感性.结果表明,AcNPV对4龄甜菜夜蛾幼虫的致死中浓度为1 ml 9.1×106 PIB(多角体),4龄幼虫摄入亚致死剂量病毒对化蛹产生不良影响,并使性比发生变化,雌雄蛹比为0.6～0.8,较对照明显升高;幼虫期摄入病毒亦对其成虫的繁殖力产生影响,无论是每雌产卵量、总卵量还是卵孵化率均比对照显著降低,交配比例和次数也比对照明显下降.甜菜夜蛾成虫期摄入病毒亦对繁殖力产生影响,产卵量和交配比例和次数以及卵孵率均比对照明显下降,此外,还对后代幼虫的存活率有显著影响.
文摘The arrangement patterns of nucleocapsids within the envelope of \%Autographa californica\% nuclear polyhedrosis virus were studied by electron microscopy. Numbers of nucleocapsids observed in an envelope in their cross sections ranged from 1 to 17, and the frequencies at 2,3,4,5,6 and 7 nucleocapsids were significantly higher than the others, suggesting that these numbers of nucleocapsids were more commonly involved in an envelope. The regular arrangement patterns of nucleocapsids within envelope were observed in cross sections. Envelope outlines can be classified into a triangle (3 nucleocapsids in an envelope),lozenge and square (4 nucleocapsids in an envelope), trapeziun (5 nucleocapsids in an envelope), pentagons (5 or 8 nucleocapsids in an envelope) and hexagons (7 or 10 nucleocapsids in an envelope). The irregular arrangement patterns of nucleocapsids within envelopes were also observed in cross sections.
