Ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS) and the MetabolynxTM software, combined with mass defect filtering, were applied to identity the metabolites of quercet...Ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS) and the MetabolynxTM software, combined with mass defect filtering, were applied to identity the metabolites of quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside(QGR) in rats after intravenous administration. MSE was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the rapid structural characterization of eight metabolites in rat plasma, urine and bile. The results indicated that methylation and glucuronidation were the major metabolic pathways of QGR in vivo. The present study provided important information about the metabolism of QGR which will be useful for fully understanding the mechanism of action of this compound. Furthermore, this work demonstrated the potential of the UPLC-Q-TOF/MS approach using Metabolynx for rapid and automated research of the metabolites of natural products.展开更多
基金supported by the National Science and Technology Support Program of China(No.2011 BAI04B03)
文摘Ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF/MS) and the MetabolynxTM software, combined with mass defect filtering, were applied to identity the metabolites of quercetin-3-O-β-D-glucopyranosyl-(4→1)-α-L-rhamnoside(QGR) in rats after intravenous administration. MSE was used for simultaneous acquisition of precursor ion information and fragment ion data at high and low collision energy in one analytical run, which facilitated the rapid structural characterization of eight metabolites in rat plasma, urine and bile. The results indicated that methylation and glucuronidation were the major metabolic pathways of QGR in vivo. The present study provided important information about the metabolism of QGR which will be useful for fully understanding the mechanism of action of this compound. Furthermore, this work demonstrated the potential of the UPLC-Q-TOF/MS approach using Metabolynx for rapid and automated research of the metabolites of natural products.
