Test Method for Evaluating the Cleaning Performance of Proteases in Automatic Dishwashing Detergent

A test method was developed to better evaluate the cleaning performance of protease in Chinese automatic dishwashing detergent(ADD)and differentiate the washing performance of different ADD formulations on protein stains.Steamed egg stain,which was a Chinese-consumers-relevant soil,was used as monitor.The response curves of the stain to different amounts of protease were measured under laboratory conditions,and the stain removal ability of commercially available ADDs was tested with this method.The results showed that the soil removal rate of ADD without protease was less than 10%,and the soil removal rate increased with the increase of protease dosage in the range of 0~2.0%.Steamed egg stain had a good response to protease,and it could effectively distinguish the cleaning performance of commercial ADDs.The test method could be used in the development,screening and evaluation of ADD formulations.

全自动免疫组化与手工染色标记胸腺瘤TdT表达的对比观察

目的:对比观察胸腺瘤Td T在全自动免疫组织化学染色技术(automatic immunohistochemical staining techniques,AIST)和手工染色方法(manual staining method,MSM)中表达的差异性。方法:收集2015年-2016年大坪医院胸腺瘤手术标本21例,经石蜡包埋、切片、染色,显微镜下观察胸腺瘤Td T在AIST和MSM表达的差异。AIST采用Ventana公司生产的BenchM ark XT,二抗检测系统分别采用UltraV iew Universal DAB Detection kit(简称UV)和Optiview DAB IHC Detection kit(简称OV)。结果:手工染色TdT阳性表达定位准确,染色强度强,背景清晰;AIST中采用UV二抗检测系统TdT染色虽然阳性定位准确,但TdT染色强度偏弱,背景不清晰,两者比较具有显著性差异(P<0.01)。优化染色方案后,改用OV二抗检测系统,TdT染色各项指标优于UV二抗检测系统,染色效果和手工相比,染色强度和阳性率一致。结论:任何抗体指标在行免疫组化前均需进行最适条件的摸索与优化,从而获得该抗体指标最佳的染色方案。

全自动免疫组织化学染色技术的应用

目的：探讨全自动免疫组织化学染色技术在基层医院的应用。方法：随机选取常见肿瘤50例，经包埋、切片后分为2组，分别采用全自动免疫组化机与手工操作2种免疫组化染色方法，显微镜下观察染色结果。结果：免疫组化机染色的组织定位准确，染色强度强，背景清晰，未见切片边缘着色现象。而手工染色虽然组织定位准确，但染色强度强弱不一，背景不清晰，存在非特异性染色，切片边缘着色现象较多，两者比较有显著性差异，均P〈0．05。结论：全自动免疫组织化学染色技术比手工操作染色效果好，易于做到染色标准化。

蛋白酶在自动洗碗剂中的应用性能测试方法研究

为了更好评估蛋白酶在中国自动洗碗剂中的应用性能,比较不同洗碗剂配方在蛋白类污渍上的去污能力,研究开发了一种与中国消费者相关的蒸蛋污渍和洗涤测试方法。在实验室条件下测试得到了该污渍对不同计量蛋白酶的响应曲线,并采用该方法测试了市售洗碗剂产品的去污力。结果表明,不含蛋白酶的洗碗剂对该污渍的去除率低于10%,蛋白酶添加量为0~2.0%,污渍去除率随着蛋白酶计量的增加而提高。该污渍对蛋白酶有很好的计量响应,能有效区分市售洗碗剂产品清洁性能,可用于洗碗剂配方的开发、筛选和去污力评价。