Selected aptamer specially combing 5-8F cells based on automatic screening instrument

Since the concept of aptamer emerged,many scientists have launched a rich field of research around it.However,few nucleic acids aptamer which use cell as target can be put into practical applications.We believe that a great deal of this lies in the complexity and irreproducibility of aptamer screening experiments themselves.The complexity is due to the cumbersome processes and the technical requirements for laboratory personnel,whereas irreproducibility arises from the fact that the starting point of such screens is nucleic acid libraries with random fragments,and that different libraries directly determine the differences or even the success or failure of screening results.The complexity and irreproducibility mentioned above,in turn,lead to the inability of this experiment to unfold on a large scale,which naturally cannot lead to excellent results for practical applications.In response to this problem,our group has developed an instrument for automated screening of tumor cell nucleic acid aptamers and characterized the properties of nucleic acid aptamers obtained using this instrument in a comprehensive manner.

Research into the inversion of the induced polarization relaxation time spectrum based on the uniform amplitude sampling method

The induced polarization relaxation time spectrum（RTS） reflects the distribution of rock pore size,which is a key factor in estimating the oil or water storage capacity of strata.However,as the data acquisition and transmission abilities of well logging instruments are much limited due to the underground environment,it is necessary to explore suitable sampling methods which can be used to obtain an accurate RST with less sampling data.This paper presents a uniform amplitude sampling method（UASM）,and compares it with the conventional uniform time sampling method（UTSM） and logarithm time sampling method（LTSM） in terms of the adaptability to different strata,RTS inversion accuracy,and stratum vertical resolution.Numerical simulation results show that the UASM can obtain high inversion accuracy of RTS with different kinds of pore size distribution formation,with high dynamic ranges of pore size,and with a small number of sampling points.The UASM,being able to adapt to the attenuation speed of polarization curve automatically,thus has the highest vertical resolution.The inversion results of rock samples also show that the UASM is superior to the UTSM and LTSM.

Automated screening of primary cell-based aptamers for targeting and therapy of pancreatic cancer

Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body.To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study.Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level.Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.