INTRODUCTION Hepatocellular carcinoma(HCC)is the mostcommon cause of death from cancer in China.Themechanisms of hepatocarcinogenesis are not yetknown clearly,p16INK4a gene,the multiple tumorsuppressor gene 1(MTS1),en...INTRODUCTION Hepatocellular carcinoma(HCC)is the mostcommon cause of death from cancer in China.Themechanisms of hepatocarcinogenesis are not yetknown clearly,p16INK4a gene,the multiple tumorsuppressor gene 1(MTS1),encodes P16 protein,which acts as an inhibitor by binding directly toCDK4 and CDK6 and preventing its association展开更多
[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple s...[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases.展开更多
AIM： To assess the three polymorphJsm regions within cytotoxic T-lymphocyte antigen 4 （CTLA-4） gene, a C/T base exchange in the promoter region-318 （CTLA-4 -318C/T）, an A/G substitution in the exon 1 position 49 ...AIM： To assess the three polymorphJsm regions within cytotoxic T-lymphocyte antigen 4 （CTLA-4） gene, a C/T base exchange in the promoter region-318 （CTLA-4 -318C/T）, an A/G substitution in the exon 1 position 49 （CTLA-4 49A/G）, a T/C substitution in 1172 （CTLA-4 -1172T/C） in patients with chronic hepatitis B. METHODS： Fifty-one patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to the hepatic clinic. Classification of chronic hepatitis B virus （HBV）-infected patients was as asymptomatic carrier state （26 patients） and chronic hepatitis B （25 patients）. Genomic DNA was isolated from anti-coagulated peripheral blood Bully coat using Miller＇s salting-out method. The presence of the CTLA-4 gene polymorphisms was determined using polymerase chain reaction amplification refractory mutation system （ARMS）. RESULTS： We observed a significant association between -318 genotypes frequency （T＋C-, T＋C＋, T-C＋） and susceptibility to chronic hepatitis B （P=0.012, OR=0.49, 95%CI： 0.206-1.162）. However, we did not observe a significant association for ＋49 genotype frequency （T＋C＋, T＋C- T-C＋） and -1172 genotype frequency （C＋T＋, T＋C- C＋T-） and state of disease. CONCLUSION： Our results suggest that CTLA-4 gene polymorphisms may partially be involved in the susceptibility to chronic hepatitis B.展开更多
Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gast...Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.展开更多
Costimulation plays very important role in T cells activation and tumor immunity. B71 is alone of the major costimulatory molecules both in human and rodents. Previous work indicated that B71 gene transfected EL4 lymp...Costimulation plays very important role in T cells activation and tumor immunity. B71 is alone of the major costimulatory molecules both in human and rodents. Previous work indicated that B71 gene transfected EL4 lymphoma can induce antitumor immunity in vivo. This paper showed that inoculation of EL4B71+ plus IL2 could elicit an antitumor effects in vivo and in vitro. Immunization with EL4B71+ tumor cells or EL4B71+ tumor cells plus IL2 could significantly prolong the survival time of the EL4 tumorbearing mice and also delay the occurrence of the tumor node in vivo. A strong proliferation response and CTL activity as well as the increased IL2 and TNF production of the splenocyte from the immunized mice with EL4B71+ or EL4B71+ plus IL2 were seen in the in vitro TLMC system. These finding indicated that the costimulatory molecule is necessary for inducing an effective antitumor immunity and IL2 optimal production.展开更多
Objective：To investigate the effect of hepatitis C virus non-structural protein 4B（HCV NS4B） on c-Myc, P53, ras gene expression＂ and apoptosis in hepatic cells and study the possible role that NS4B played in the c...Objective：To investigate the effect of hepatitis C virus non-structural protein 4B（HCV NS4B） on c-Myc, P53, ras gene expression＂ and apoptosis in hepatic cells and study the possible role that NS4B played in the carcinogenesis of heparoma. Methods： The recombinant plasmid（PCXN2-NS4B, PCXN2-P53） and the empty, vector were transfected or co-transfected into Chang liver cells with liposome. Screening was performed with G418. Plasmid mRNA was detected by RT-PCR. The pro rein expressions of c-Myc and ras genes were analyzed by immunocytochemistry. The expressions of wild-type P53 （wtp53） gene were detected by in situ hybridization. TUNEL（flow cytometry） was used for assessing the rate of apoptosis. Results：No expression of c-Myc gene was found in PCXN2 group. The expression of c-Myc gene in NS4B group was 21.3% ＋ 1.2%. The ex pression of ras gene in PCXN2 group was lower than that in NS4B group. Compared with PCXN2 group, the expression of P53 mRNA was not promoted or inhibited in NS4B group. But the expression of P53 mRNA in NS4B-P53 group was lower than that in P53 group. In PCXN2, NS4B, P53 and NS4B-P53 group, the rates of apoptosis were 17.02% ± 1.24%, 11.94% ± 2.24%, 25.84% ± 3.49% and 18.34% ± 1.55% respectively. Conclusion ：HCV NS4B induces the expression of c-Myc and ras gene. HCV NS4B may play a role in the inhibition of cell death through P53-dependent manner. Results from this study suggested that HCV NS4B might contribute to the viral carcinogenesis.展开更多
Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 ...Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 patients with chronic hepatitis B virus(HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene(rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio(OR) [95% confidence interval(CI)] = 1.070(0.793-1.445); rs2243283, P = 0.849, OR(95% CI) = 0.976(0.758-1.257); rs2243288, P = 0.659, OR(95% CI) = 1.060(0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups. Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.展开更多
基于家蚕微孢子虫(Nosema bombycis,Nb)基因组中发现的蓖麻毒素B链(ricin B chain)序列数据设计引物,提取感染Nb第8天的家蚕幼虫中肠组织总RNA,利用RT-PCR方法扩增出RBL463-4基因,并将其克隆进毕赤酵母表达载体pPICZα-A中,构建重组酵...基于家蚕微孢子虫(Nosema bombycis,Nb)基因组中发现的蓖麻毒素B链(ricin B chain)序列数据设计引物,提取感染Nb第8天的家蚕幼虫中肠组织总RNA,利用RT-PCR方法扩增出RBL463-4基因,并将其克隆进毕赤酵母表达载体pPICZα-A中,构建重组酵母表达载体pPIC-R4。重组质粒pPIC-R4经SacⅠ线性化后,电击导入毕赤酵母X33中,利用博莱霉素(zeocin)筛选获得重组酵母X33/RBL463-4。以2%甲醇诱导表达后,经SDS-PAGE、Western blot检测表达产物,结果出现25kD和20kD2种蛋白,其中25kD蛋白与预测融合蛋白大小一致,表明获得了RBL463-4的融合蛋白。研究结果为进一步探究RBL463-4蛋白在家蚕微孢子虫侵染宿主过程中的功能作用奠定了基础。展开更多
Objective To investgate the metabolism of terephthalic acid （TPA） in rats and its mechanism. Methods Metabolism was evaluated by incubating sodium terephthalate （NaTPA） with rat normal liver microsomes, or with mi...Objective To investgate the metabolism of terephthalic acid （TPA） in rats and its mechanism. Methods Metabolism was evaluated by incubating sodium terephthalate （NaTPA） with rat normal liver microsomes, or with microsomes pretreated by phenobarbital sodium, or with 3-methycholanthrene, or with diet control following a NADPH-generating system. The determination was performed by high performance liquid chromatography （HPLC）, and the mutagenic activation was analyzed by umu tester strain Salmonella typhimurium NM2009. Expression of CYP4B 1 mRNA was detected by RT-PCR. Results The amount of NaTPA （12.5-200 μmol-L^1） detected by HPLC did not decrease in microsomes induced by NADPH-generating system. Incubation of TPA （0.025-0.1 mmol-L^-1） with induced or noninduced liver microsomes in an NM2009 umu response system did not show any mutagenic activation. TPA exposure increased the expression of CYP4B1 mRNA in rat liver, kidney, and bladder. Contusion Lack of metabolism of TPA in liver and negative genotoxic data from NM2009 study are consistent with other previous short-term tests, suggesting that the carcinogenesis in TPA feeding animals is not directly interfered with TPA itself and/or its metabolites.展开更多
Objective: To study differentially expressed genes by silencing cyclin B1, and to sift out autophagy-related genes. Methods: Double thymidine deoxyribonucleoside blocking was used to synchronize nasopharyngeal carcino...Objective: To study differentially expressed genes by silencing cyclin B1, and to sift out autophagy-related genes. Methods: Double thymidine deoxyribonucleoside blocking was used to synchronize nasopharyngeal carcinoma cell (CNE-2) to S phase, then flow cytometry was applied to test transfection efficiency. The mRNA and protein expression level of cyclin B1 was assessed by q-PCR and western blot, respectively. Differentially expressed genes were screened by high-throughput gene chip. Results: Double thymidine deoxyribonucleoside (2.5 mmol/L) blocking was used to synchronize the cell cycle to S phase. The transfection efficiency of CNE-2 cells was 85.6%. Compared with negative group,cyclin B1-siRNA treated group significantly down-regulated mRNA expression of cyclin B1 (80%) and protein level (75.3%). Totally, 2408 differentially expressed genes were found in CNE-2, including 1245 up-regulated genes and 1163 down-regulated genes. Moreover, PTEN, an autophagy-related gene, was preliminarily sifted out. Conclusions: Cyclin B1-siRNA significantly down-regulated the expression of cyclin B1 and yielded a total of 2408 differentially expressed genes, including PETN (an autophagy-related gene).展开更多
目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，...目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，分离白细胞，提取基因组DNA，采用特异性引物PCR方法，扩增LAPTM4B第一外显子5′UTR内的部分序列，对三组人群进行分析研究．x^2检验分析肝癌组与对照组LAPTM4B的基因多态性和其他相关因素的相关性．结果：LAPTM4B等位基因在三组观察对象中的分布，^＊1和^＊2在健康对照组的频率分别是75．71％和24．29％，慢肝组73．16％和26、84％，肝癌组中66．84％和33.45％，三组比较等位基因分布频率有统计学意义，健康对照组与肝癌组比较有统计学意义（x^2=6．979，P=0．008）．LAPTM4B的基因型LAPTM4B1^＊1型、LAPTM4B^＊1／2混合型和LAPTM482^＊2型在肝癌组中的频率分别是37.9％、57.9％和4．2％、慢肝组50．5％、45．3％和4．2％、健康对照组56．6％、38.3％和5．1％，三组间三种基因型分布频率比较有统计学意义（x^2=14．854，P〈0．005）.结论：基因型^＊1／2和等位基因^＊2可能与肝癌的发生有关．展开更多
基金the National Natural Science Foundation of China,No.39670702
文摘INTRODUCTION Hepatocellular carcinoma(HCC)is the mostcommon cause of death from cancer in China.Themechanisms of hepatocarcinogenesis are not yetknown clearly,p16INK4a gene,the multiple tumorsuppressor gene 1(MTS1),encodes P16 protein,which acts as an inhibitor by binding directly toCDK4 and CDK6 and preventing its association
基金Supported by Agricultural Independent Innovation Fund of Jiangsu Province[CX(09)638]~~
文摘[Objective] This study aimed to clone C4B gene in Siji goose and detect its expression level in different tissues. [Method] cDNA sequence of C4B gene was cloned with RACE-PCR method. Amino acid sequences in multiple species were aligned in GenBank, and a phylogenetic tree was constructed for homology analysis. [Result] C4B gene in Siji goose shared relatively high homology with chicken and quail; Siji goose C4B gene was expressed highly in liver and lung of adult geese and expressed lowly in epididymis, seminiferous duct, brain, kidney, testis, heart, oviduct and smal intestine. [Conclusion] In the present study, mRNA expression lev-el of C4B gene in different tissues and organs of Siji goose was determined by flu-orescence quantitative PCR, which provided basis for rapid diagnosis of specific an-imal diseases.
文摘AIM： To assess the three polymorphJsm regions within cytotoxic T-lymphocyte antigen 4 （CTLA-4） gene, a C/T base exchange in the promoter region-318 （CTLA-4 -318C/T）, an A/G substitution in the exon 1 position 49 （CTLA-4 49A/G）, a T/C substitution in 1172 （CTLA-4 -1172T/C） in patients with chronic hepatitis B. METHODS： Fifty-one patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to the hepatic clinic. Classification of chronic hepatitis B virus （HBV）-infected patients was as asymptomatic carrier state （26 patients） and chronic hepatitis B （25 patients）. Genomic DNA was isolated from anti-coagulated peripheral blood Bully coat using Miller＇s salting-out method. The presence of the CTLA-4 gene polymorphisms was determined using polymerase chain reaction amplification refractory mutation system （ARMS）. RESULTS： We observed a significant association between -318 genotypes frequency （T＋C-, T＋C＋, T-C＋） and susceptibility to chronic hepatitis B （P=0.012, OR=0.49, 95%CI： 0.206-1.162）. However, we did not observe a significant association for ＋49 genotype frequency （T＋C＋, T＋C- T-C＋） and -1172 genotype frequency （C＋T＋, T＋C- C＋T-） and state of disease. CONCLUSION： Our results suggest that CTLA-4 gene polymorphisms may partially be involved in the susceptibility to chronic hepatitis B.
基金supported by the Fundamental Research Funds for the Central Universities (WUT:2020IB029)。
文摘Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.
文摘Costimulation plays very important role in T cells activation and tumor immunity. B71 is alone of the major costimulatory molecules both in human and rodents. Previous work indicated that B71 gene transfected EL4 lymphoma can induce antitumor immunity in vivo. This paper showed that inoculation of EL4B71+ plus IL2 could elicit an antitumor effects in vivo and in vitro. Immunization with EL4B71+ tumor cells or EL4B71+ tumor cells plus IL2 could significantly prolong the survival time of the EL4 tumorbearing mice and also delay the occurrence of the tumor node in vivo. A strong proliferation response and CTL activity as well as the increased IL2 and TNF production of the splenocyte from the immunized mice with EL4B71+ or EL4B71+ plus IL2 were seen in the in vitro TLMC system. These finding indicated that the costimulatory molecule is necessary for inducing an effective antitumor immunity and IL2 optimal production.
基金Scientific Research Fund of Sichuan Provincial Education Department(20003531)
文摘Objective：To investigate the effect of hepatitis C virus non-structural protein 4B（HCV NS4B） on c-Myc, P53, ras gene expression＂ and apoptosis in hepatic cells and study the possible role that NS4B played in the carcinogenesis of heparoma. Methods： The recombinant plasmid（PCXN2-NS4B, PCXN2-P53） and the empty, vector were transfected or co-transfected into Chang liver cells with liposome. Screening was performed with G418. Plasmid mRNA was detected by RT-PCR. The pro rein expressions of c-Myc and ras genes were analyzed by immunocytochemistry. The expressions of wild-type P53 （wtp53） gene were detected by in situ hybridization. TUNEL（flow cytometry） was used for assessing the rate of apoptosis. Results：No expression of c-Myc gene was found in PCXN2 group. The expression of c-Myc gene in NS4B group was 21.3% ＋ 1.2%. The ex pression of ras gene in PCXN2 group was lower than that in NS4B group. Compared with PCXN2 group, the expression of P53 mRNA was not promoted or inhibited in NS4B group. But the expression of P53 mRNA in NS4B-P53 group was lower than that in P53 group. In PCXN2, NS4B, P53 and NS4B-P53 group, the rates of apoptosis were 17.02% ± 1.24%, 11.94% ± 2.24%, 25.84% ± 3.49% and 18.34% ± 1.55% respectively. Conclusion ：HCV NS4B induces the expression of c-Myc and ras gene. HCV NS4B may play a role in the inhibition of cell death through P53-dependent manner. Results from this study suggested that HCV NS4B might contribute to the viral carcinogenesis.
基金Supported by Grants from the National Natural Science Foundationof China,No.81072342the National Pre-973 Program Projects,No.2009CB526411
文摘Objective To investigate the relationship between single nucleotide polymorphisms(SNPs) of the interleukin-4(IL-4) gene and outcome of hepatitis B virus(HBV) infection in a Chinese Han population.Methods Total of 501 patients with chronic hepatitis B virus(HBV) infection and 301 controls with selflimiting HBV infection were studied. Three tag SNPs in the IL-4 gene(rs2227284G/T, rs2243283C/G and rs2243288A/G) were genotyped by the Multiplex snapshot technique. The genotype and allele frequencies were calculated and analyzed.Results The three SNPs showed no significant genotype/allele associations with chronic HBV infection. Overall allele P values were: rs2227284, P = 0.655, odds ratio(OR) [95% confidence interval(CI)] = 1.070(0.793-1.445); rs2243283, P = 0.849, OR(95% CI) = 0.976(0.758-1.257); rs2243288, P = 0.659, OR(95% CI) = 1.060(0.818-1.375). Overall genotype P values were: rs2227284, P = 0.771; rs2243283, P = 0.571; rs2243288, P = 0.902. There were no statistically significant differences between patients with chronic HBV infection and controls. Haplotypes generated by these three SNPs also had no significant differences between the two groups. Conclusions The three tag SNPs of IL-4 were not associated with the outcome of HBV infection in the Han Chinese population.
文摘基于家蚕微孢子虫(Nosema bombycis,Nb)基因组中发现的蓖麻毒素B链(ricin B chain)序列数据设计引物,提取感染Nb第8天的家蚕幼虫中肠组织总RNA,利用RT-PCR方法扩增出RBL463-4基因,并将其克隆进毕赤酵母表达载体pPICZα-A中,构建重组酵母表达载体pPIC-R4。重组质粒pPIC-R4经SacⅠ线性化后,电击导入毕赤酵母X33中,利用博莱霉素(zeocin)筛选获得重组酵母X33/RBL463-4。以2%甲醇诱导表达后,经SDS-PAGE、Western blot检测表达产物,结果出现25kD和20kD2种蛋白,其中25kD蛋白与预测融合蛋白大小一致,表明获得了RBL463-4的融合蛋白。研究结果为进一步探究RBL463-4蛋白在家蚕微孢子虫侵染宿主过程中的功能作用奠定了基础。
基金This work was supported by the National Natural Science Foundation of China (No. 30170798), the Preliminary Study of an ImportantProject in the National Basic Research, MOST (No. 2001CCA04900) and the Greatest Project in the National Basic Research (No.2002CB512908).
文摘Objective To investgate the metabolism of terephthalic acid （TPA） in rats and its mechanism. Methods Metabolism was evaluated by incubating sodium terephthalate （NaTPA） with rat normal liver microsomes, or with microsomes pretreated by phenobarbital sodium, or with 3-methycholanthrene, or with diet control following a NADPH-generating system. The determination was performed by high performance liquid chromatography （HPLC）, and the mutagenic activation was analyzed by umu tester strain Salmonella typhimurium NM2009. Expression of CYP4B 1 mRNA was detected by RT-PCR. Results The amount of NaTPA （12.5-200 μmol-L^1） detected by HPLC did not decrease in microsomes induced by NADPH-generating system. Incubation of TPA （0.025-0.1 mmol-L^-1） with induced or noninduced liver microsomes in an NM2009 umu response system did not show any mutagenic activation. TPA exposure increased the expression of CYP4B1 mRNA in rat liver, kidney, and bladder. Contusion Lack of metabolism of TPA in liver and negative genotoxic data from NM2009 study are consistent with other previous short-term tests, suggesting that the carcinogenesis in TPA feeding animals is not directly interfered with TPA itself and/or its metabolites.
基金This study was funded by the Natural Science Foundation of Fujian Province(2015J01457).
文摘Objective: To study differentially expressed genes by silencing cyclin B1, and to sift out autophagy-related genes. Methods: Double thymidine deoxyribonucleoside blocking was used to synchronize nasopharyngeal carcinoma cell (CNE-2) to S phase, then flow cytometry was applied to test transfection efficiency. The mRNA and protein expression level of cyclin B1 was assessed by q-PCR and western blot, respectively. Differentially expressed genes were screened by high-throughput gene chip. Results: Double thymidine deoxyribonucleoside (2.5 mmol/L) blocking was used to synchronize the cell cycle to S phase. The transfection efficiency of CNE-2 cells was 85.6%. Compared with negative group,cyclin B1-siRNA treated group significantly down-regulated mRNA expression of cyclin B1 (80%) and protein level (75.3%). Totally, 2408 differentially expressed genes were found in CNE-2, including 1245 up-regulated genes and 1163 down-regulated genes. Moreover, PTEN, an autophagy-related gene, was preliminarily sifted out. Conclusions: Cyclin B1-siRNA significantly down-regulated the expression of cyclin B1 and yielded a total of 2408 differentially expressed genes, including PETN (an autophagy-related gene).
文摘目的：探讨溶酶体相关4次跨膜蛋白质B（lysosome-associated protein transmembrane 4 beta，LAPTM4B）基因多态性与肝癌易感性的关系．方法：应用病例对照研究方法，收集190例肝癌患者、190例慢性乙型肝炎患者、175例健康献血者全血，分离白细胞，提取基因组DNA，采用特异性引物PCR方法，扩增LAPTM4B第一外显子5′UTR内的部分序列，对三组人群进行分析研究．x^2检验分析肝癌组与对照组LAPTM4B的基因多态性和其他相关因素的相关性．结果：LAPTM4B等位基因在三组观察对象中的分布，^＊1和^＊2在健康对照组的频率分别是75．71％和24．29％，慢肝组73．16％和26、84％，肝癌组中66．84％和33.45％，三组比较等位基因分布频率有统计学意义，健康对照组与肝癌组比较有统计学意义（x^2=6．979，P=0．008）．LAPTM4B的基因型LAPTM4B1^＊1型、LAPTM4B^＊1／2混合型和LAPTM482^＊2型在肝癌组中的频率分别是37.9％、57.9％和4．2％、慢肝组50．5％、45．3％和4．2％、健康对照组56．6％、38.3％和5．1％，三组间三种基因型分布频率比较有统计学意义（x^2=14．854，P〈0．005）.结论：基因型^＊1／2和等位基因^＊2可能与肝癌的发生有关．
