Isolation and the Full-length Genome Sequencing of Subgroup J Avian Leukosis Virus ZH-08 Isolate Associated with Hemangioma

A subgroup J avian leukosis virus (AVL-J), designated as ZH-08, was isolated from a breeder flock in Guangdong province with a novel hemangioma case. The identification results of ELISA test, PCR and immunofluoresecence assay (IFA) specific for ALV-J were all positive. Based on the public full-length proviral genome sequence of ALV-J prototype strain HPRS-103, three pairs of primers were synthesized. The full-length proviral genome sequence of ZH-08 isolate is 7597 bp, which has a little difference with that of published full-length genome sequences, but its organization corresponds with typical retroviral genome structure; and known oncogenes were not included in its genome. According to the gp85 sequence comparison of ZH-08 isolate with those of the other reference strains in China and abroad, the highest similarity (93.7%) was with the YZ9901 isolate. Phylogenetic analysis, based on the gp85 gene, showed that the ZH-08 isolated here had the closest linkage to the SD07LK1 isolate. This study provides the basis for the biological characterization and pathogenesis research of the ZH-08 isolate.

Isolation and Identification of a Subgroup A Avian Leukosis Virus from Imported Meat-type Grand-parent Chickens

An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that SDAU09C1 fell into subgroup A; it had homology of 88.8%-90.3% to 6 reference strains of subgroup A, much higher compared to other subgroups including subgroup B. This is the first report for ALV of subgroup A isolated from imported breeders.

Establishment of an avian leukosis virus subgroup A-resistant cell line

Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A （ALV-A） env gene into plasmid pcDNA3.1/Zeo （＋） and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCIDso/0.1 mL （50% tissue culture infective dose）ALV-A and was partially resistant to 10~ TCIDs0/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISAfor capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool.

J、K亚群禽白血病病毒的分离和囊膜蛋白(gp85)基因序列分析

为了解现阶段我国禽白血病病毒(ALV)的流行特征和演化趋势,本试验于2021—2022年从国内某鸡场采集蛋清ALV衣壳蛋白(p27)抗原阳性的鸡血浆,接种鸡胚成纤维细胞(DF-1)分离病毒并进行亚群鉴定;采用聚合酶链式反应(PCR)技术扩增分离株的囊膜蛋白(gp85)基因片段,并对其进行测序,将基因序列与ALV不同亚群毒株进行比对和遗传进化分析;选取氨基酸变化差异较大的分离毒株进行间接免疫荧光鉴定。结果显示,共分离到7株ALV毒株(3株J亚群,4株K亚群),ALV-J分离毒株CAU4932、CAU4860和CAU2259的gp 85基因片段长度为918 bp,编码306个氨基酸,分离毒株之间gp 85基因核苷酸同源性为90.5%~95.2%,gp85蛋白氨基酸同源性为95.1%~99.3%;ALV-K分离毒株CAU7049、CAU5006、CAU7176和CAU7168的gp 85基因片段长度为1008 bp,编码336个氨基酸,分离毒株之间gp 85基因核苷酸同源性为89.4%~92.3%,gp85蛋白氨基酸同源性为94.0%~99.7%。gp85蛋白氨基酸序列同源性比对发现,分离株gp85蛋白氨基酸序列均会发生突变,但高变区hr1和hr2所占比例较多,证实了gp85的高变性。在gp85蛋白B细胞抗原表位中存在部分位点的氨基酸突变,可能导致gp85蛋白抗原性发生变化。间接免疫荧光鉴定结果显示,5株分离毒株均可在DF-1细胞内复制并存在可被抗体识别的p27抗原表位。结果表明,本试验分离的7株ALV毒株是国内ALV-J和ALV-K流行毒株的突变株,不仅丰富了ALV基因组库资源,且为后续研究ALV的遗传变异特征和流行趋势等提供参考依据。

禽白血病病毒J亚群感染鸡组织中细胞受体chNHE 1的表达分析

旨在研究鸡钠氢交换蛋白1(chicken sodium hydrogen exchanger isoform 1,chNHE1)在不同类型鸡组织内的表达情况,J亚群禽白血病病毒(subgroup J avian leukosis virus, ALV-J)的组织嗜性及ALV-J感染后组织chNHE1的mRNA变化情况。本研究利用qPCR技术,检测了商品肉鸡、商品蛋鸡和SPF鸡脏器内的chNHE1转录量,ALV-J感染鸡后各组织的病毒载量及组织chNHE1的转录量的变化。结果显示,chNHE1在3种类型鸡的心、肝、脾、肺、肾、盲肠扁桃体、胸腺和法氏囊中均能检测到,但转录量高低存在较大差异,其中,免疫器官内的chNHE1转录量相对较高;SPF鸡接种ALV-J后,心、脾、肾和盲肠扁桃体中的病毒载量相对较高,而肝、肺、胸腺和法氏囊中的病毒载量相对较低;ALV-J感染后,脾、肾和盲肠扁桃体中的chNHE1转录量明显上调,但心中的转录量与空白对照相比无明显变化。本研究分析了3种类型鸡chNHE1的组织转录特点及ALV-J感染对组织内chNHE1转录的影响,该研究结果为探究受体chNHE1的转录与ALV-J的组织嗜性的关系提供重要的理论依据。

Evolution of gp85 gene of subgroup J avian leukosis virus under the selective pressure of antibodies

Subgroup J Avian leucosis virus (ALV-J) strain NX0101 was inoculated into chicken embryo fibroblasts (CEF) monolayers in 6-well plates. The six wells of CEF inoculated with NX0101 were divided into groups A (without anti-ALV-J serum in the medium); B (with anti-ALV-J serum in the medium), then viruses from each well of both groups were separately passed in CEF every 6 d; formed their independent passage lineages. For each lineage of both groups, gp85 genes of the viruses in the 10th, 20th; 30th passages were amplified, cloned; sequenced. The sequence data indicated that the homologies of gp85 at aa level between the primary virus; the passed viruses of different passages of 3 lineages in group A were 97.7%–99.7%;; the homologies of gp85 between the primary virus; the passed viruses of different passages of 3 lineages in group B were 93.8%–96.1%. Analysis of the ratios of nonsynonium (NS) vs synonium (S) mutations of nucleic acids demonstrated that NS/S in 3 highly variable (hr-) regions at aa#110–120, aa#141–151; aa#189–194 of gp85 in 3 lineages of group A were 2 (8/4), 1(3/3); 1.3 (4/3), however, NS/S in the same 3 hr-regions of group B were 4.1 (13/3), 4.7 (14/3); 3.3 (11/3). This study is the first demonstration of influence of immune selective pressure on evolution of ALV-J gp85 by specific antibodies under the controlled in vitro experiments.

Different quasispecies with great mutations hide in the same subgroup J field strain of avian leukosis virus

Blood samples were collected from a local strain of chickens associated with serious tumor cases in Shandong Province. The samples were inoculated into chicken embryo fibroblast and DF-1 cells for virus isolation and identification, respectively. The inoculated cells were screened for three common chicken tumor viruses. Nine strains of avian leukosis virus subgroup J （ALV-J） were identified, and were designated LY1201-LYI209. The env gene from the LY1201 strain was amplified and cloned. All nine resultant env clones （clones 01-09） were sequenced, and the gp85 and gp37 amino acid regions were subjected to homology analysis. Clones 01 and 03 had 10 amino acid deletions in the gp85 region compared to the other seven clones, suggesting that at least two quasispecies with obvious mutations coexist in the same field strain. Among these nine clones, three had identical gp85 and gp37 sequences, and were recognized as the dominant LY1201 quasispecies. The amino acid sequence homology of gp37 and gp85 among the nine clones was 98.5%-100.0% and 96.6%-100.0% respectively, suggesting that the gp85 region of the env gene can better display the quasispecies diversity of ALV-J than gp37.

Influence of REV and ALV-J Co-Infection on Immunologic Function of T Lymphocytes and Histopathology in Broiler Chickens

The aim of present investigation is to study the effect of single- and co-infection with REV and ALV-J on T lymphocytes bioactivities and histopathology in broiler chickens. The bioactivities of blood and spleen T lymphocytes including lymphoproliferation responses, cytotoxicitic responses, and histopathology of spleen were detected in broiler chickens singly- or co-infected with REV and ALV-J at different days post inoculation and the virus expressions in spleen of infected broiler chickens were detected with immunofluorescence assay （IFA）. The results indicated that blood and spleen T lymphocytes proliferation responses and cytotoxicity in broilers infected with REV or/and ALV-J were inhibited in the whole observed period compared with controls. In the co-infected chickens they were highly inhibited than in the single-infected. The histopathology of spleen in infected chickens at 17 and 37 d post inoculation （dpi） indicated that cell interium increased, the numbers of lymphocytes decreased, and the regrowth were destroyed or decreased, especially more significantly at 17 than at 37 dpi. The different numbers of virus were detected in spleen lymphocytes in REV- infected and/or ALV-J-infected chickens. In the spleen of co-infected chicken, both REV and ALV-J were detected and the total numbers of viruses were more than in chickens singly-infected with REV or ALV-J. Thus, the co-effect of REV and ALV-J caused more immunosuppression on T lymphocytes bioactivities in broiler chickens than single-effect of ALV-J or REV, which contributed to the sever histopathology and the product of tumor cells. This study will be helpful for understanding the effect of co-infection with many viruses and control them in poultry.

竹醋液抑制J亚群禽白血病病毒复制研究

为探讨竹醋液在体外对J亚群禽白血病病毒(Avian leukosis virus subgroup J,ALV-J)复制的影响,研究首先通过CCK-8试剂盒测定青皮竹和慈竹两种竹醋液对DF-1细胞毒性,随后将不同浓度竹醋液与ALV-J等体积室温作用不同时间后接种到细胞中,利用qRTPCR、ELISA和Western blot测定竹醋处理对ALV-J编码基因的转录以及蛋白合成的影响。结果显示:两种竹醋液加入细胞中的最终浓度均≤2%,对细胞生长没有影响;青皮竹醋和慈竹醋在100%、50%、25%的浓度下与病毒在室温作用10 min、30 min后,细胞中gp37基因表达水平显著下降,细胞与上清中的P27蛋白表达水平也明显下降。研究表明,青皮竹醋和慈竹醋在体外均有明显的抗ALV-J活性。

鸡IFIT5对J亚群禽白血病病毒在DF-1细胞内复制的影响

【目的】制备鸡干扰素诱导蛋白含三角形四肽重复5(IFIT5)多克隆抗体,以探究IFIT5对J型禽白血病病毒(ALV-J)在鸡胚成纤维细胞(DF-1)内复制的影响。【方法】以感染ALV-J的鸡脾脏组织细胞提取的RNA反转录获得的cDNA作为模板,对IFIT5基因进行PCR扩增,利用重组酶将纯化后的PCR产物和线性化载体进行连接,构建重组质粒pET-28a-IFIT5和pcDNA5-flag-IFIT5并测序。将测序正确的重组质粒pET-28a-IFIT5转化大肠杆菌BL21感受态细胞进行诱导表达,表达的融合蛋白His-IFIT5纯化后免疫6周龄BALB/c雌鼠制备多克隆抗体,通过Western blotting鉴定抗体的特异性,利用间接ELISA测定抗体的效价。将测序正确的重组质粒pcDNA5-flag-IFIT5转染DF-1细胞,24 h后接种ALV-J,通过Western blotting检测DF-1细胞中过表达IFIT5对ALV-J复制的影响。【结果】试验成功扩增到大小为1 440 bp的IFIT5基因,并成功构建重组质粒pET-28a-IFIT5和pcDNA5-flag-IFIT5。pET-28a-IFIT5可以表达约56 ku的可溶性蛋白His-IFIT5,Western blotting结果显示,制备的鼠抗IFIT5蛋白多克隆抗体能特异性识别DF-1细胞中过表达的IFIT5蛋白,效价为1∶32 000。pcDNA5-flag-IFIT5能在DF-1细胞中高效表达,且在DF-1细胞中过表达IFIT5可以促进ALV-J复制。【结论】本研究制备的鼠抗IFIT5蛋白多克隆抗体具有较高的反应性和特异性;在DF-1细胞中过表达IFIT5可以促进ALV-J复制。本试验结果为深入研究IFIT5蛋白的生物学功能提供参考。

Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus

Subgroup J avian leukosis virus(ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles(EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles(SE) were isolated and purified from ALV-J-infected rooster seminal plasma(SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection.More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission.

1株J亚群禽白血病病毒env基因克隆及生物信息学分析

【目的】通过生物信息学方法分析1株禽白血病病毒(Avian Leukosis virus,ALV)的囊膜蛋白env的分子特征。【方法】利用鸡成纤维细胞(DF-1)培养疑似禽白血病病鸡的血清样品,通过ELISA和实时荧光定量PCR鉴定ALV亚群。对env基因PCR产物进行测序,利用SeqMan拼接env基因。将该env基因与国内外多个ALV亚群进行序列比对及遗传进化分析,并借助多种生物信息学软件对env蛋白进行分析,包括理化性质、亲/疏水性、跨膜区、信号肽、糖基化位点、磷酸化位点、乙酰化位点、二级结构、三级结构、抗原决定簇和B细胞抗原表位预测。【结果】ALV-J毒株的env基因全长1719 bp,获得GenBank登录号为OP837418,其与J亚群ALV位于同一进化分支,相似性为89.3%~94.8%。该env蛋白有2个跨膜区,是由572个氨基酸组成的不稳定的亲水蛋白,不稳定系数预测值为43.49,总平均亲水性为―0.201;该env蛋白为非分泌蛋白,无信号肽,有17个N-糖基化修饰位点、77个O-糖基化修饰位点、42个磷酸化修饰位点、21个潜在抗原决定簇和9个连续碱基数超过10个的B细胞线性表位。该env蛋白的二级结构中无规则卷曲占比最大,为38.29%。【结论】env基因是导致ALV遗传变异的关键基因,其编码的囊膜蛋白env具有不稳定性,存在多个蛋白修饰位点和抗原表位。

鸡抗ALV-J感染的先天免疫应答途径研究进展

J亚群禽白血病病毒(Subgroup J avian leukosis virus,ALV-J)是禽外源性逆转录病毒,主要诱发肿瘤性疾病和免疫抑制,ALV-J在所有禽白血病病毒亚群中具有最强的致病性和传染性,给我国的养禽业造成了巨大的经济损失。由于宿主对病毒难以产生有效的免疫应答,加之病毒的基因组变异频率较快,至今为止,既没有有效的疫苗可以预防,也没有有效的药物可以治疗。只能通过病原检测、淘汰阳性鸡、净化种群来防控。本文对宿主抵抗ALV-J感染的先天免疫应答途径进行了概括总结,阐述ALV-J的免疫抑制机制、宿主的先天免疫系统和抗病毒感染的相关信号通路,并对ALV-J未来的相关研究方向作了展望,以期为该病的研究和防控提供理论参考。

用ALV-Jgp85单克隆抗体证明蛋鸡存在J亚群禽白血病

采用免疫组化法,对病理学初步诊断为蛋用型鸡 J亚群白血病的自然发病鸡的肿瘤、骨髓、肝脏、脾脏、肾脏、肺脏、心脏、胰脏、输卵管、卵巢、腺胃、骨骼肌、大脑、坐骨神经,用特异性抗 J亚群禽白血病病毒(ALV J)囊膜糖蛋白gp85的单克隆抗体进行检测,待检的组织切片中均检出阳性抗原,免疫组化的研究结果与病理学诊断结果相一致。在国内外首次发现并报道蛋用型鸡 J亚群禽白血病的自然病例。

我国部分地区蛋鸡群ALV-J及与REV、MDV、CAV混合感染检测

为了解J亚群禽白血病病毒(ALV-J)及其与禽网状内皮细胞增生症病毒(REV)、马立克氏病病毒(MDV)和鸡传染性贫血病病毒(CAV)的混合感染现象,本研究从宁夏、湖北、广东、山东、辽宁、吉林、黑龙江7个省的39个蛋鸡群收集临床表现和剖检病理变化疑似禽白血病的病料样品184份,采用PCR、病毒分离和IFA检测样品中ALV-J、REV、MDV和CAV。结果表明,7个省蛋鸡场均存在ALV-J感染,病料样品阳性率为60.9%,检测鸡群阳性率为82.1%,与REV、MDV、CAV的混合感染率分别为13.6%、24.5%、22.8%,其中存在较为严重的双重感染(29.0%)和3重感染(18.8%),甚至4重感染(1.7%)。研究结果表明,我国蛋鸡群中普遍存在ALV-J感染,而且与REV、MDV、CAV混合感染严重;提示ALV-J已经可以引起蛋鸡群发病,在临床诊断和致病性研究中,应考虑到多重感染的影响。

贵州省禽白血病病毒J亚群感染的血清学检测与PCR鉴定

采用禽白血病J亚群抗体检测试剂盒对8个鸡场的血清样本进行了检测,并对出现肿瘤病变的病死鸡进行了病理组织学检查;同时采集16份禽白血病病毒J亚群(ALV-J)抗体阳性鸡的血液或肝组织,提取DNA样本进行PCR扩增,并测序分析。结果显示,4个肉种鸡场的ALV-J抗体阳性率平均为16.52%(3.33%～30.0%),而4个蛋种鸡场的ALV-J抗体阳性率均为0。病理组织学观察可见,在肝、肾、脾组织中出现大量增生的、含嗜酸性颗粒的髓细胞样瘤细胞。PCR检测发现,有14份样本扩增出ALV-J亚群特异性的病原核酸片段(545 bp),检出率达87.5%,其中在3份肝样本中同时扩增出ALV-A亚群特异性的病原核酸片段(691 bp);而ALV-J抗体阴性鸡、淋巴细胞性白血病和血管瘤型白血病的20份样本均未扩增出545 bp片段。扩增出的545 bp片段与ALV-J亚群毒株HPRS-103和ADOL-HcL的相应核苷酸序列的同源性分别达96.2%～96.8%和95.5%～96.8%。结果证实,贵州省鸡群中确实存在ALV-J亚群感染和骨髓细胞瘤病,且出现A亚群、J亚群混合感染的情况。

地方柴鸡中J亚群禽白血病与马立克氏病的混合感染

2008年9月,山东省菏泽市某柴鸡养殖专业户6 000只鸡80日龄时开始发病,至120日龄时死亡率高达15%。病鸡颜面苍白,机体进行性消瘦,瘫痪,最后衰竭死亡。部分鸡只可见单侧肢体麻痹症状和皮肤型肿瘤。对66份采自发病鸡群的血清及新生羽毛囊进行血清学检测。琼脂扩散试验结果显示所采的66份待检羽髓抗原MDV阳性率为59.09%,血清中MDV抗体阳性率为65.16%,抗原、抗体双阳性率为37.88%。采用酶联免疫吸附试验(ELISA)检测鸡血清中ALV-J、REV抗体,结果显示ALV-J抗体阳性率为7.58%,REV抗体检测全部呈阴性。剖检病鸡可见内脏器官普遍肿大,肝、脾、肾表面及切面有大小不等的灰白色肿瘤样结节。病理组织学观察肿大的器官和组织内都有不同程度的淋巴细胞增生,并可在肝、脾、肾、腺胃等组织内同时观察到淋巴细胞样瘤细胞和髓样瘤细胞2种典型的肿瘤灶。免疫组织化学检测结果表明,发病鸡组织内同时存在MDV和ALV-J抗原的阳性信号。PCR检测结果显示6只来检病鸡MDV和ALV-J均呈阳性,都扩增出相应的条带,REV未扩增出任何条带,PCR呈阴性。以上结果表明地方柴鸡鸡群中存在MDV和ALV-J的共感染,提醒我们应当注意地方柴鸡肿瘤性疾病的预防和净化工作。

鸡感染J亚群禽白血病病毒的免疫抑制机理

通过人工接种建立了雏鸡感染J亚群禽白血病病毒(ALV-J)后发生免疫抑制的病理模型,对免疫抑制的机理进行了初步研究。结果表明:ALV-J感染早期可诱导胸腺、法氏囊的淋巴细胞凋亡,这种早期的淋巴细胞凋亡是胸腺和法氏囊萎缩的重要原因之一。病理组织学动态观察表明,ALV-J主要引起骨髓的髓系细胞灶状或弥漫性增生,病变导致骨髓机能受损,使机体免疫机能下降,是引起免疫抑制的根本原因。病毒感染组免疫器官均发生了严重的实质萎缩性病变,这种病变的发生除与骨髓的病变及淋巴细胞凋亡有关外,还与中后期淋巴细胞的坏死有关,从而导致严重的免疫抑制。ALV-J感染可引起免疫器官及部分内脏器官中嗜酸性粒细胞样的瘤细胞浸润增生,这可以作为病毒感染早期的病理诊断指标。

817肉杂鸡肉瘤组织分离出A、J亚型禽白血病病毒

山东某地饲养的36日龄817肉杂鸡生长迟缓并发生颈部肉瘤。取肉瘤组织上清液接种CEF,培养12d,用IFA试验进行病毒鉴定。检测结果表明,从该肉瘤组织分离到A亚型与J亚型禽白血病病毒(ALV-A、ALV-J),命名为SD1005株。取肉瘤组织上清液人工感染817肉杂鸡与SPF鸡进行动物回归试验,结果复制出同样肉瘤。用复制出的新鲜肉瘤分别制备肉瘤细胞与肉瘤组织触片,IFA试验再次验证出ALV-A与ALV-J均为阳性,而且发现ALV-A与ALV-J分别共感染同一个组织细胞。同时,通过PCR方法用针对ALV-A、ALV-J的2对引物扩增并鉴定出肉瘤组织中含有ALV-A、ALV-J。对扩增的ALV-J囊膜糖蛋白(env)基因1 710bp片段进行的遗传变异分析结果显示,SD1005株与14株国内外参考株之间的同源性为88.5%～94.0%,其中与来自白羽肉鸡的美国参考株0661(AF247566)、ADOL-Hc1(AF097731)的同源性最高,分别为94.0%、93.8%。本研究显示,来自817肉杂鸡的肉瘤组织上清液接种鸡可诱发急性肉瘤,其中既检出ALV-A,又检出ALV-J,但急性肉瘤由单个病毒诱发还是与共感染相关有待于进一步研究。

J亚群禽白血病病毒SCAU-0901株的分离鉴定

在组织病理学观察的基础上,经接种DF-1细胞系、ELISA抗原检测、聚合酶链式反应(PCR)以及亚群特异性免疫荧光试验(IFA),从送检的疑似禽白血病感染的麻黄肉种鸡中分离并鉴定出1株J亚群禽白血病病毒(ALV-J),命名为SCAU-0901。利用特异性引物分别扩增出长度为545 bp和2.2×103bp的目的片段,并对目的片段进行了克隆测序。序列分析发现,SCAU-0901株的gp85基因与国内外各ALV-J毒株相应核苷酸序列的相似性为86.1%～95.1%,其中与BJ0303株的相似性最高(95.1%),与ADOL-7501株的相似性最低(86.1%)。系统遗传进化分析表明,SCAU-0901株的gp85基因片段与BJ0303株的亲缘关系最近。