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禽类呼肠孤病毒(Avian reoviruses)感染
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作者 吴异健 刘文兴 吴宝成 《福建畜牧兽医》 2004年第z1期13-15,共3页
  1病原学   1.1病毒特征禽类呼肠孤病毒为无囊膜,呈球形,双层衣壳和二十面对称的dsRNA病毒.粒子直径约60~80 nm,外层衣壳上有92颗中空的颗粒.在CsCl梯度离心中,感染病毒子的密度为1.29~1.30g/ml,无感染性空衣壳为1.29~1.30g/ml...   1病原学   1.1病毒特征禽类呼肠孤病毒为无囊膜,呈球形,双层衣壳和二十面对称的dsRNA病毒.粒子直径约60~80 nm,外层衣壳上有92颗中空的颗粒.在CsCl梯度离心中,感染病毒子的密度为1.29~1.30g/ml,无感染性空衣壳为1.29~1.30g/ml,病毒芯髓为1.44 g/ml.纯化病毒只含有RNA和蛋白质,平均含量分别为18.7%和81.3%.病毒在胞浆内复制,有时呈晶格排列.对热、pH 3和DNA代谢抑制物有抵抗力.MgCl2能增强病毒对热的稳定性,但当浓度太大时反而促其灭活.70%乙醇和0.5%有机碘可灭活病毒.一般无血凝活性.   …… 展开更多
关键词 呼肠孤病毒 致病性 临床症状 火鸡 呼吸道肠道孤儿病毒 RNA病毒 avian reoviruses 禽类 病毒毒力
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Circulation of Immunosuppressive Viruses and Avian Encephalomyelitis Virus in Backyard Chicken Flocks 被引量:1
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作者 Priscila de Castro Almeida Pricila Ribeiro Silva Borges +6 位作者 Priscilla K.Koerich Roberta Torres de Melo Igor Alves Batista Eliane Pereira Mendonca Rogério Reis Silva Lillian Karla Silva Belchiolina Beatriz Fonseca 《Advances in Microbiology》 2020年第5期203-213,共11页
The objective of this study was to evaluate the circulation of Chicken Anemia Virus (CAV), Infectious Bursal Disease Virus (IBDV), Avian Reovirus (ARV) and Avian Encephalomyelitis virus (AEV) in properties of backyard... The objective of this study was to evaluate the circulation of Chicken Anemia Virus (CAV), Infectious Bursal Disease Virus (IBDV), Avian Reovirus (ARV) and Avian Encephalomyelitis virus (AEV) in properties of backyard chickens and carry out an epidemiological analysis. We evaluated 200 samples of chickens from 19 backyard chicken property. Only one property (P10) did not present serological titers for the diseases evaluated. This property is close to industrial farms as well as the other properties, however, P10 remained a few years without the breeding of chicks and these were the first poultry to be housed on site. This reinforces the importance of the fallow period for poultry production. The prevalence of virus-seroreactive birds was 78% (156/200), 64.5% (129/200), 78% (156/200), 78% (156/200) for CAV, IBDV, ARV and, EA, respectively. All the free-range farms studied are within a radius of 500 meters to 6 Km away from some establishments of industrial poultry. There was a correlation between serological titers for CAV and the frequency of disease in poultry (r = 0.6178). In places where birds are frequently sick, 30.76% reported that the disease occurs in chicks, 30.76% in broilers, 23.07% in broiler chickens and 7.69% in birds of all ages. Birds get sick more often in the summer period. The owners reported that the most common signs of disease were respiratory signs (snoring and nasal discharge) (46.15%), diarrhea (30.76%), and paralysis of wings and/or paws (38.46%). There was a correlation between the presence of untreated water in the property and serological titers for ARV (r = 0.5576). This report draws attention not only to high serological prevalence for the viruses studied but also important epidemiological aspects of backyard chicken diseases that may indirectly influence the industrial production. 展开更多
关键词 Chicken Anemia Virus Infectious Bursal Disease Virus avian Reovirus EPIDEMIOLOGY
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Effect of Maternal Antibodies on the Pathogenesis of Avian Reovirus Infections in Broiler Chickens Using Real-Time Reverse Transcriptase Polymerase Chain Reaction
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作者 Kejun Guo Teresa Dormitorio +1 位作者 Shan-Chi Ou Joseph Giambrone 《Journal of Agricultural Science and Technology(A)》 2012年第9期1058-1063,共6页
The effect of maternal antibodies on the pathogenesis of avian reovirus (ARV) was studied in commercial and specific pathogen free broilers (SPF) using a real-time reverse transcriptase (RT)-polymerase chain rea... The effect of maternal antibodies on the pathogenesis of avian reovirus (ARV) was studied in commercial and specific pathogen free broilers (SPF) using a real-time reverse transcriptase (RT)-polymerase chain reaction (PCR) assay, along with the incidence and severity of morbidity, mortality, and gross lesions. ARV RNA was detected in cloacal swabs in both bird groups from the first day throughout the 21 days experiment. Commercial broiler chickens, which had high maternal antibodies against ARV, showed minimum clinical signs, gross lesions, and lower numbers of birds with viral RNA excretion, whereas specific pathogen free (SPF) broiler chickens, which did not have antibody against ARVs, had 30% mortality, more severe signs, and higher numbers of birds excreting viral RNA. The highest peak of SPF birds excreting viral RNA occurred during the time of maximum mortality. The protective effect of maternal antibody on ARV pathogenesis in broiler chickens correlated with the detection of ARV RNA using the real-time RT-PCR. 展开更多
关键词 PATHOGENESIS ANTIBODY avian reovirus real-time RT-PCR.
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Construction of Short Hairpin RNA Vector with σNS & σC Genes of Avian Reovirus and Determination of Interference Effect
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作者 XIONG Wen-jie XIE Zhi-xun +4 位作者 LIU Jia-bo PANG Yao-shan XIE Zhi-qin DENG Xian-wen XIE Li-ji 《Animal Husbandry and Feed Science》 CAS 2012年第3期133-137,共5页
[ Objective] The aim was to explore novel method for treatment of Avian Reovirus. [ Method] According to the design principle of siRNA target sequences, siRNA templates were designed and synthesized and then cloned in... [ Objective] The aim was to explore novel method for treatment of Avian Reovirus. [ Method] According to the design principle of siRNA target sequences, siRNA templates were designed and synthesized and then cloned into the shRNA expression vector, namely, pSilencer-CMV 4.1 neo. Short hairpin RNA vector C1, C2, C3, which contain σC gene, and shRNA vector NS1, NS2, NS3, which contain aNS gene, were constructed separately. The constructed shRNA vectors and negative control were co-transfected into DF-1 cells with the eukaryotic expression vector pEG- FP-σC and pEGFP-σNS, respectively. [ Result] Observation through fluorescence microscope indicated that the constructed 6 shRNA could inhibit the expression of fusion protein to different degrees. In addition, results of Real-time PCR suggested that C3 and NS1 have the best interference effect to the viral duplication in vitro. [ Conclusionl Construction and selection of specific shRNA expression vectors inhibiting Avian Reovirus are significant for researching effects of σC and oNS proteins in infection and duplication of ARV, providing new idea for ARV antiviral therapy. 展开更多
关键词 avian Reovirus Short Hairpin RNA INTERFERENCE
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Sequence and phylogenetic analysis of chicken reoviruses in China
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作者 WEN Chu ZHONG Qi +6 位作者 ZHANG Jia-dong LU Jian-shan ZHANG Li-xin YUAN Xi-min GAN Meng-hou CAI Xue-peng ZHANG Guo-zhong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第8期1846-1855,共10页
supported by the China Animal Disease Prevention and Control Center;the China Agriculture Research System Poultry-Related Science and Technology Innovation Team of Peking, China (CARS-PSTP)
关键词 avian reovirus chicken genome S1 homology phylogenetic analysis pathogenicity
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