The Characterization of Steam Distillation as an Extraction Method to Extract Volatile Compounds from <i>Prunella vulgaris</i>and the Investigation of Their Anti-Tumorous Effect

Prunella vulgaris (PV) is a perennial plant which is widely grown around the world. It has been widely used as a medicinal treatment for generations. Previous studies showed extracts from this plant had a wide range of therapeutic efficacy, including anti-tumorous effect. However, the volatile organic compounds (VOCs) extracted from it were rarely explored. This paper reports on the characterization of a steam distillation process to extract VOCs in PV and also the anti-tumorous effects of the PV distillate using the tetrazolium-based Cell Counting Kit-8 (CCK-8) as the test agent, when the VOCs were used to treat oral squamous cancer cells, SSC154. It was found that most abundant VOCs came out steadily and continuously for as long as the duration of the steam extraction could extend. However, some compounds such as benzaldehyde did show depletion as the distillation process progressed, while some compounds such as caryophyllene oxide was only sparsely found at the beginning of distillation. The PV distillate was mildly effective in its cytotoxicity to cancer cells SCC154, in a dosage dependent manner.

共沸蒸馏GC/MS测定非卤代挥发性有机物的方法研究

讨论了用共沸蒸馏法浓缩处理水、土壤、固废样品或其萃取液中不易吹除、具挥发性、且易溶于水的非卤代挥发性有机物,并用气相色谱质谱联用仪定性定量的方法。

全二维气相色谱-飞行时间质谱法分析广藿香浸膏中挥发性有机物

应用全二维气相色谱-飞行时间质谱法(GC×GC-TOFMS)分析广藿香浸膏的挥发性有机物。采用超临界CO2流体萃取广藿香样品获得广藿香浸膏,浸膏经分子蒸馏分离,所得的轻馏分经GC×GC-TOFMS分析。二维色谱由Rxi-5sil MS柱和Rtx-200柱串联而成,通过TOFMS软件结合质谱分析对化合物进行定性定量分析。分析得到匹配度大于750的组分有323个,含量占总量的89.45%,其中酮类67个、醇类52个、烯类40个、酸类37个、烷类36个、酯类34个、醛类17个,其它类40个。相对含量大于0.1%的组分69个,其中相对含量大于1%的组分11个,广藿香酮和广藿香醇为主要成分,质量分数分别为21.75%和18.93%。