Comprehensive Summary Pd-mediated bioorthogonal cleavage reactions have been extensively utilized in the activation of prodrug molecules,precise regulation of protein function,and cellular engineering.However,the avai...Comprehensive Summary Pd-mediated bioorthogonal cleavage reactions have been extensively utilized in the activation of prodrug molecules,precise regulation of protein function,and cellular engineering.However,the availability of cleavable"caging"groups is quite limited,and their application in nucleic acid modification has seldom been reported.Herein,we introduce a method based on Pd-catalyzed reduction amination of azides as a decaging strategy to activate the activity of biomolecules.We designed modifications on the bioactive sites with azides or their derivatives to mask the related biological function,followed by the release of biological activity through Pd-catalyzed NaBH4 reduction amination reaction.This study has demonstrated that the strategy can effectively be used to activate bioactive molecules such as fluorescent probes,prodrugs,and to regulate the biological function of RNA,including reverse transcription extension,binding to ligands,and cleavage activity of the CRISPR-Cas system.All results confirm that this strategy provides an efficient and controllable"OFF to ON"biological switch,capable of achieving significant regulatory effects substoichiometrically,and is expected to be extended to other biological applications.展开更多
This paper is concerned with the synthesis of poly (ether-urethane) with pendant amino groups. The copolyether produced by ring opening copolymerization of tetrahydrofuran and epibromohydrin was treated with sodium az...This paper is concerned with the synthesis of poly (ether-urethane) with pendant amino groups. The copolyether produced by ring opening copolymerization of tetrahydrofuran and epibromohydrin was treated with sodium azide in dimethylformamide to form azido substituted copolyether. The poly (ether-urethane) made from it was subjected to reduction reaction to convert azido group to amino group. The stress-strain behavior and dynamic-mechanical properties of poly (ether-urethane) containing pendant amino groups were studied.展开更多
基金the National Natural Science Foundation of China(Nos.22177089,21721005,92153303,22037004,22177088)the Fundamental Research Funds for the Central Universities(2042021kf0211)Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(Grant No.ZNJC202309).
文摘Comprehensive Summary Pd-mediated bioorthogonal cleavage reactions have been extensively utilized in the activation of prodrug molecules,precise regulation of protein function,and cellular engineering.However,the availability of cleavable"caging"groups is quite limited,and their application in nucleic acid modification has seldom been reported.Herein,we introduce a method based on Pd-catalyzed reduction amination of azides as a decaging strategy to activate the activity of biomolecules.We designed modifications on the bioactive sites with azides or their derivatives to mask the related biological function,followed by the release of biological activity through Pd-catalyzed NaBH4 reduction amination reaction.This study has demonstrated that the strategy can effectively be used to activate bioactive molecules such as fluorescent probes,prodrugs,and to regulate the biological function of RNA,including reverse transcription extension,binding to ligands,and cleavage activity of the CRISPR-Cas system.All results confirm that this strategy provides an efficient and controllable"OFF to ON"biological switch,capable of achieving significant regulatory effects substoichiometrically,and is expected to be extended to other biological applications.
文摘This paper is concerned with the synthesis of poly (ether-urethane) with pendant amino groups. The copolyether produced by ring opening copolymerization of tetrahydrofuran and epibromohydrin was treated with sodium azide in dimethylformamide to form azido substituted copolyether. The poly (ether-urethane) made from it was subjected to reduction reaction to convert azido group to amino group. The stress-strain behavior and dynamic-mechanical properties of poly (ether-urethane) containing pendant amino groups were studied.
