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Sensitive determination of DNA based on resonance light scattering enhancement of azocarmine G and CTAB
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作者 陈展光 任凤莲 +3 位作者 司士辉 廖栩泓 丁卫锋 刘锦斌 《Journal of Central South University of Technology》 EI 2005年第6期688-692,共5页
A new method for the determination of DNA was developed with azocarmine G(AG) in the presence of cetyltrimethylammonium bromide(CTAB) by the resonance light scattering (RLS) technique. The synthetic samples were deter... A new method for the determination of DNA was developed with azocarmine G(AG) in the presence of cetyltrimethylammonium bromide(CTAB) by the resonance light scattering (RLS) technique. The synthetic samples were determined with satisfactory results, and the reaction mechanism was also studied. The results show that underthe optimum conditions, the weak RLS signal of AG can be enhanced by DNA, which results from the formation of a new ternary complex AG-CTAB-DNA with large size. Moreover, the enhanced RLS intensity at 552 nm is directlyproportional to the concentration of DNA in the range of 01.0 μg/mL for fish sperm DNA (fsDNA) and 01.5 μg/mLfor calf thymus DNA (ctDNA). Based on this, a new assay of DNA can be established. The detection limits (3σ) are 2.1 ng/mL for fsDNA and 2.2 ng/mL for ctDNA, respectively. 展开更多
关键词 DETERMINATION resonance light scattering azocarmine G DNA cetyltrimethylammonium bromide
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Determination of trace amounts of silver(I) in the presence of an activator with a kinetic method 被引量:1
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作者 Shahryar Abbasi Hadi Barzegaramiri Abbas Farmany 《Rare Metals》 SCIE EI CAS CSCD 2014年第6期731-736,共6页
A simple and sensitive kinetic-spectrophotometric method was developed for the determination of trace amounts of silver, based on its catalytic effect on the oxidation of azocarmine G by peroxydisulfate in sulfuric ac... A simple and sensitive kinetic-spectrophotometric method was developed for the determination of trace amounts of silver, based on its catalytic effect on the oxidation of azocarmine G by peroxydisulfate in sulfuric acid medium and at 30 °C in the presence of 1,10-phenantroline as activator. The absorbance was measured at 525 nm with the fixed-time method. The optimization of the operating conditions regarding concentrations of the reagents, temperature, ionic strength, and interferences was also investigated. The calibration curve is linear over the concentration range 0.3–50.0 ng ml-1of silver with good precision and accuracy, and the detection limit is down to0.125 ng ml-1. The relative standard deviation for a standard solution of 25.0 ng ml-1of silver is 1.36 %(n = 10).The proposed method proves to be highly sensitive,selective, and relatively rapid for the assay of silver at lowlevel range of 0.3–50.0 ng ml-1without any pre-concentration and separation step. The method was applied for the determination of silver in river water and white photographic film. The analytic results of the real samples are in excellent agreement with atomic absorption spectrometry analysis. The analytical results obtained are satisfactory. 展开更多
关键词 SILVER Kinetic-catalytic determination azocarmine G ACTIVATOR 1 10-Phenantroline
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