A flagellar gene cluster fragment includingflbD of Azospirillum brasilense was cloned and sequenced. TheflbD mutant strain was found to be nonmotile——losingboth polar and lateral flagella (Fla-Laf-). Motility and fl...A flagellar gene cluster fragment includingflbD of Azospirillum brasilense was cloned and sequenced. TheflbD mutant strain was found to be nonmotile——losingboth polar and lateral flagella (Fla-Laf-). Motility and fla-gella were regained by complementation with plasmid-borne multicopy flbD, but altered with larger swarming circle and fewer lateral flagella on the semisolid plate. This result indicated that FlbD plays an important role in the regulation of both polar and lateral flagellar biosynthesis in A. brasilense.展开更多
基金This work was supported by the National "863" High-tech Project (Grant No. 863-101-03-04-02).
文摘A flagellar gene cluster fragment includingflbD of Azospirillum brasilense was cloned and sequenced. TheflbD mutant strain was found to be nonmotile——losingboth polar and lateral flagella (Fla-Laf-). Motility and fla-gella were regained by complementation with plasmid-borne multicopy flbD, but altered with larger swarming circle and fewer lateral flagella on the semisolid plate. This result indicated that FlbD plays an important role in the regulation of both polar and lateral flagellar biosynthesis in A. brasilense.
