The X gene of HBV encodes a 17-kD protein, termed HBx, which has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of this study was to ...The X gene of HBV encodes a 17-kD protein, termed HBx, which has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of this study was to investigate the effect of HBx on gene expression of interleukin （IL）-1β and IL-6, and proliferation of rat mesangial cells in vitro. The X gene of HBV was amplified by PCR assay, and inserted into the eukaryotic expression vector pCI-neo. The structure of recombinant pCI-neo-X plasmid was proved by restrict endonuclease digestion and sequencing analysis. pCI-neo-X was transfected into cultured rat mesangial cell line in vitro via liposome. HBx expression in transfected mesangial cells was detected by Western blot. The IL-1β and IL-6 mRNA expression in those cells was assayed by semiquantitative RT-PCR. Mesangial cell proliferation was tested by MTT. The results showed that HBx was obviously expressed in cultured mesangial cell line at 36th and 48th h after transfection. The expression of IL-1β and IL-6 mRNA was simultaneously increased. The cell proliferation was also obvious at the same time. It was concluded that HBx gene transfection could induce IL-1β and IL-6 gene expression and mesangial cell proliferation. HBx may play a critical role in mesangial cell proliferation through upregulation of the IL-1β and IL-6 gene expression.展开更多
目的:探讨新疆维吾尔族、汉族弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者Bcl-6、c-myc基因易位的差异及其临床意义。方法:采用荧光免疫原位杂交(FISH)方法,对233例DLBCL活体石蜡切片进行Bcl-6、c-myc基因检测。观察Bc...目的:探讨新疆维吾尔族、汉族弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者Bcl-6、c-myc基因易位的差异及其临床意义。方法:采用荧光免疫原位杂交(FISH)方法,对233例DLBCL活体石蜡切片进行Bcl-6、c-myc基因检测。观察Bcl-6、c-myc基因易位与DLBCL患者临床资料的关系,并对不同民族在不同亚型DLBCL中Bcl-6、c-myc基因易位的情况进行比较。结果:233例DLBCL中,Bcl-6基因重排51例,占21.89%;c-myc基因重排39例,占16.74%;Bcl-6基因易位的表达与患者年龄、性别、发病部位、临床分期和LDH水平无显著相关(P>0.05),而与民族、IPI评分、结外侵犯、B症状、DLBCL不同亚型和近期疗效有相关性(P<0.05);c-myc基因易位的表达与患者年龄、性别、发病部位、临床分期、LDH水平和DLBCL不同亚型无明显相关性(P>0.05),而与民族、IPI评分、结外侵犯、B症状和近期疗效有相关性(P<0.05);维、汉不同民族GCB中Bcl-6、c-myc基因易位比较差异均无统计学意义(P>0.05);维、汉不同民族非生发中心活化B细胞(non-GCB)中Bcl-6、c-myc基因易位比较差异有统计学意义(P<0.05)。结论:Bcl-6,C-myc基因易位的表达与维、汉不同民族、IPI评分、结外侵犯、B症状和近期疗效有相关性;维、汉民族non-GCB亚组中Bcl-6、c-myc基因易位存在差异。展开更多
目的研究3q27染色体断裂及bcl-6基因扩增与弥漫性大B细胞淋巴瘤（diffuse large B-cell lymphoma, DLBCL）与其分子分类及治疗效果、临床分期的关系。方法用细胞芯片荧光原位杂交（fluomscence in situ hybridization, FISH）技术对60例...目的研究3q27染色体断裂及bcl-6基因扩增与弥漫性大B细胞淋巴瘤（diffuse large B-cell lymphoma, DLBCL）与其分子分类及治疗效果、临床分期的关系。方法用细胞芯片荧光原位杂交（fluomscence in situ hybridization, FISH）技术对60例DLBCL的标本进行3q27染色体断裂及bcl-6扩增检测；采用免疫组化S-P法在组织微阵列上同步观测CD20、CD10、bel-6、MUM1的表达，进行生发中心样（genninat center B-cell-like，GCB）和非生发中心样（non-germinal center B-cell．like，non-GCB）分子分类；通过对临床病例的分析得出与治疗效果及临床分期的信息；统计分析以上各因素之间的关系。结果在60例DLBCL中，GCB占48．3％（29／60），non-GCB占51．7％（31／60）。FISH结果显示，3q27断裂阳性15例，bcl-6基因扩增阳性22例。存在3q27染色体断裂的15例中BCL-6蛋白表达阳性3例（20．0％），阴性12例（80．0％），与无3q27染色体断裂者相比其BCL-6蛋白表达率降低（P=0．017）。在60例DLBCL中，bcl-6扩增22例，其中GCB5例（22．7％），non-GCB17例（77．3％），与无bcl-6扩增者相比差异有统计学意义（P=0．003）。在36例经正规CHOP治疗的DLBCL中，bcl-6扩增15例，其治疗效果显效、部分有效、无效分别为4（26．7％）、4（26．7％）、7（46．7％），与无bcl-6扩增的病例比差异有统计学意义（P=0．016）。bcl-6扩增与BCL-6蛋白表达及临床分期的关系差异无统计学。BCL-6蛋白表达阳性组、阴性组与治疗效果及临床分期关系差异无统计学意义。结论存在bcl-6基因断裂的病例，其BCL-6蛋白表达率低。存在bcl-6基因扩增的DLBCL多数为non-GCB，并且治疗效果差，临床分期较晚，可能与DLBCL晚期染色体呈多倍体增加的趋势有关。展开更多
基金a grant from National Natural Science Foundation of China (No. 30772360)
文摘The X gene of HBV encodes a 17-kD protein, termed HBx, which has been shown to function as a transcriptional trans-activator of a variety of viral and cellular promoter/enhancer elements. The aim of this study was to investigate the effect of HBx on gene expression of interleukin （IL）-1β and IL-6, and proliferation of rat mesangial cells in vitro. The X gene of HBV was amplified by PCR assay, and inserted into the eukaryotic expression vector pCI-neo. The structure of recombinant pCI-neo-X plasmid was proved by restrict endonuclease digestion and sequencing analysis. pCI-neo-X was transfected into cultured rat mesangial cell line in vitro via liposome. HBx expression in transfected mesangial cells was detected by Western blot. The IL-1β and IL-6 mRNA expression in those cells was assayed by semiquantitative RT-PCR. Mesangial cell proliferation was tested by MTT. The results showed that HBx was obviously expressed in cultured mesangial cell line at 36th and 48th h after transfection. The expression of IL-1β and IL-6 mRNA was simultaneously increased. The cell proliferation was also obvious at the same time. It was concluded that HBx gene transfection could induce IL-1β and IL-6 gene expression and mesangial cell proliferation. HBx may play a critical role in mesangial cell proliferation through upregulation of the IL-1β and IL-6 gene expression.
文摘目的:探讨新疆维吾尔族、汉族弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)患者Bcl-6、c-myc基因易位的差异及其临床意义。方法:采用荧光免疫原位杂交(FISH)方法,对233例DLBCL活体石蜡切片进行Bcl-6、c-myc基因检测。观察Bcl-6、c-myc基因易位与DLBCL患者临床资料的关系,并对不同民族在不同亚型DLBCL中Bcl-6、c-myc基因易位的情况进行比较。结果:233例DLBCL中,Bcl-6基因重排51例,占21.89%;c-myc基因重排39例,占16.74%;Bcl-6基因易位的表达与患者年龄、性别、发病部位、临床分期和LDH水平无显著相关(P>0.05),而与民族、IPI评分、结外侵犯、B症状、DLBCL不同亚型和近期疗效有相关性(P<0.05);c-myc基因易位的表达与患者年龄、性别、发病部位、临床分期、LDH水平和DLBCL不同亚型无明显相关性(P>0.05),而与民族、IPI评分、结外侵犯、B症状和近期疗效有相关性(P<0.05);维、汉不同民族GCB中Bcl-6、c-myc基因易位比较差异均无统计学意义(P>0.05);维、汉不同民族非生发中心活化B细胞(non-GCB)中Bcl-6、c-myc基因易位比较差异有统计学意义(P<0.05)。结论:Bcl-6,C-myc基因易位的表达与维、汉不同民族、IPI评分、结外侵犯、B症状和近期疗效有相关性;维、汉民族non-GCB亚组中Bcl-6、c-myc基因易位存在差异。
文摘目的研究3q27染色体断裂及bcl-6基因扩增与弥漫性大B细胞淋巴瘤（diffuse large B-cell lymphoma, DLBCL）与其分子分类及治疗效果、临床分期的关系。方法用细胞芯片荧光原位杂交（fluomscence in situ hybridization, FISH）技术对60例DLBCL的标本进行3q27染色体断裂及bcl-6扩增检测；采用免疫组化S-P法在组织微阵列上同步观测CD20、CD10、bel-6、MUM1的表达，进行生发中心样（genninat center B-cell-like，GCB）和非生发中心样（non-germinal center B-cell．like，non-GCB）分子分类；通过对临床病例的分析得出与治疗效果及临床分期的信息；统计分析以上各因素之间的关系。结果在60例DLBCL中，GCB占48．3％（29／60），non-GCB占51．7％（31／60）。FISH结果显示，3q27断裂阳性15例，bcl-6基因扩增阳性22例。存在3q27染色体断裂的15例中BCL-6蛋白表达阳性3例（20．0％），阴性12例（80．0％），与无3q27染色体断裂者相比其BCL-6蛋白表达率降低（P=0．017）。在60例DLBCL中，bcl-6扩增22例，其中GCB5例（22．7％），non-GCB17例（77．3％），与无bcl-6扩增者相比差异有统计学意义（P=0．003）。在36例经正规CHOP治疗的DLBCL中，bcl-6扩增15例，其治疗效果显效、部分有效、无效分别为4（26．7％）、4（26．7％）、7（46．7％），与无bcl-6扩增的病例比差异有统计学意义（P=0．016）。bcl-6扩增与BCL-6蛋白表达及临床分期的关系差异无统计学。BCL-6蛋白表达阳性组、阴性组与治疗效果及临床分期关系差异无统计学意义。结论存在bcl-6基因断裂的病例，其BCL-6蛋白表达率低。存在bcl-6基因扩增的DLBCL多数为non-GCB，并且治疗效果差，临床分期较晚，可能与DLBCL晚期染色体呈多倍体增加的趋势有关。
