单纯疱疹病毒感染后脑脊液S100B、Cys-C、MMP-9水平对自身免疫性脑炎的预测价值

目的:探究单纯疱疹病毒(HSV)感染后脑脊液(CSF)中S100B、Cys-C、MMP-9水平对自身免疫性脑炎(AE)的预测价值。方法:选取2016年1月至2021年3月河北中石油中心医院收治的200例HSV感染患者为研究对象,根据是否继发AE分为研究组(继发AE,35例)和对照组(未继发AE,165例)。多因素Logistic回归分析HSV感染患者继发AE的独立影响因素。Spearman法分析脑脊液中Cys-C、MMP-9与S100B水平的相关性。受试者工作特征(ROC)曲线分析S100B、Cys-C、MMP-9对AE的预测价值。构建风险预测模型并进行评价。结果:多因素Logistic回归分析显示,MRI异常、脑脊液S100B、MMP-9升高、EEG异常是HSV感染患者继发AE的独立危险因素,脑脊液Cys-C是其保护因素(P<0.05)。Spearman分析显示,HSV感染患者Cys-C浓度与S100B水平呈负相关(r=-0.83,P<0.05),MMP-9浓度与S100B水平呈正相关(r=0.88,P<0.05)。构建的联合预测因子pre1诊断HSV患者继发AE的AUC明显大于S100B、Cys-C、MMP-9单独预测的AUC(0.876 vs 0.827、0.787、0.750)。构建的风险预测模型具有良好的区分度和一致性。结论:脑脊液中S100B、Cys-C、MMP-9水平均可对HSV感染患者诱发AE的可能性进行有效预测,且三项指标联合预测价值最大,其次是S100B蛋白、Cys-C、MMP-9。

老年脊柱骨折伴脊髓损伤患者早期血清pNF-H、NSE、S100B、C反应蛋白动态变化及意义

目的探讨老年脊柱骨折伴脊髓损伤患者早期血清神经丝蛋白H磷酸化亚型(pNF-H)、神经元特异性烯醇化酶(NSE)、S100B钙结合蛋白(S100B)、C反应蛋白动态变化特点及意义。方法老年脊柱骨折患者120例根据脊髓损伤神经功能评估结果分为脊髓未损伤组83例,脊髓不完全损伤组24例,脊髓完全损伤组13例。均行前路减压内固定术治疗,分别于脊柱骨折后12、24 h、7、14 d抽取空腹外周静脉血5 ml,酶联免疫吸附试验检测血清pNF-H、NSE、S100B、C反应蛋白水平。结果脊髓完全损伤组和脊髓不完全损伤组脊髓损伤后12、24 h、7 d血清pNF-H、NSE、S100B、C反应蛋白浓度逐渐升高,脊髓损伤后14 d下降;脊髓未损伤组脊髓损伤后12、24 h、7、14 d血清pNF-H、NSE、S100B、C反应蛋白浓度无明显变化。脊髓完全损伤组脊髓损伤后12、24 h、7、14 d血清pNF-H、NSE、S100B、C反应蛋白浓度明显高于脊髓不完全损伤组和脊髓未损伤组,脊髓不完全损伤组明显高于脊髓未损伤组(P<0.05)。受试者工作特征(ROC)曲线分析显示,血清pNF-H、NSE、S100B、C反应蛋白对老年脊柱骨折伴脊髓损伤均具有较高的诊断价值,其中血清pNF-H、NSE、S100B、C反应蛋白联合检测的诊断价值最高[曲线下面积(AUC)=0.857],灵敏度87.348%、特异度83.581%,高于各指标单独检测。结论pNF-H、NSE、S100B、C反应蛋白4项血清学指标联合检测在脊柱骨折伴脊髓损伤早期诊断和病情严重程度评估中具有较高的应用价值。

内蒙古根河三道桥铅锌银矿床C-H-O-S同位素和U-Pb定年研究及其意义

内蒙古根河三道桥大型铅锌银矿床位于大兴安岭得尔布干成矿带中北段,矿体主要呈脉状赋存于火山岩地层中。氢氧同位素研究表明,成矿期石英和绢云母的δD值变化范围为-149.1‰~-156.7‰,δ^(18)OH_(2)O值变化范围为-13.6‰~3.4‰;成矿后期石英δD值变化范围为-131.9‰~-147.7‰,δ^(18)OH_(2)O值变化范围为-16.5‰~-18.2‰。碳同位素分析结果表明,与矿化有关的方解石δ^(13)C值变化范围为-1.8‰~-3.1‰,δ^(18)O值变化范围为5.3‰~8.6‰。原位S同位素分析结果表明,硫化物的δ^(34)S值变化范围为2.3‰~5.6‰,与其西南侧下护林矽卡岩型铅锌银矿床中的硫化物的δ^(34)S值(1.2‰~5.9‰)基本一致。上述同位素组成特征指示成矿物质主要来源于岩浆热液,在上升到地壳浅部时有一定量的大气降水混入。锆石U-Pb年代学研究表明,矿化的闪长玢岩脉年龄为(136.0±0.7)Ma(MSWD=0.44);未矿化、穿切硫化物微细脉的闪长玢岩脉的锆石U-Pb年龄为(120.8±0.6)Ma(MSWD=0.49)。结合前人相关研究进展,认为三道桥铅锌银矿床形成于136.0~120.8 Ma期间(早白垩世),为伸展构造背景下与浅成侵入岩有关的中温热液型铅锌银矿床。

Gut microbiota dysbiosis contributes toα-synuclein-related pathology associated with C/EBPβ/AEP signaling activation in a mouse model of Parkinson’s disease

Parkinson’s disease is a neurodegenerative disease characterized by motor and gastrointestinal dysfunction.Gastrointestinal dysfunction can precede the onset of motor symptoms by several years.Gut microbiota dysbiosis is involved in the pathogenesis of Parkinson’s disease,whether it plays a causal role in motor dysfunction,and the mechanism underlying this potential effect,remain unknown.CCAAT/enhancer binding proteinβ/asparagine endopeptidase(C/EBPβ/AEP)signaling,activated by bacterial endotoxin,can promoteα-synuclein transcription,thereby contributing to Parkinson’s disease pathology.In this study,we aimed to investigate the role of the gut microbiota in C/EBPβ/AEP signaling,α-synuclein-related pathology,and motor symptoms using a rotenone-induced mouse model of Parkinson’s disease combined with antibiotic-induced microbiome depletion and fecal microbiota transplantation.We found that rotenone administration resulted in gut microbiota dysbiosis and perturbation of the intestinal barrier,as well as activation of the C/EBP/AEP pathway,α-synuclein aggregation,and tyrosine hydroxylase-positive neuron loss in the substantia nigra in mice with motor deficits.However,treatment with rotenone did not have any of these adverse effects in mice whose gut microbiota was depleted by pretreatment with antibiotics.Importantly,we found that transplanting gut microbiota derived from mice treated with rotenone induced motor deficits,intestinal inflammation,and endotoxemia.Transplantation of fecal microbiota from healthy control mice alleviated rotenone-induced motor deficits,intestinal inflammation,endotoxemia,and intestinal barrier impairment.These results highlight the vital role that gut microbiota dysbiosis plays in inducing motor deficits,C/EBPβ/AEP signaling activation,andα-synuclein-related pathology in a rotenone-induced mouse model of Parkinson’s disease.Additionally,our findings suggest that supplementing with healthy microbiota may be a safe and effective treatment that could help ameliorate the progression of motor deficits in patients with Parkinson’s disease.

Pancreatic surgery and tertiary pancreatitis services warrant provision for support from a specialist diabetes team

Pancreatic surgery units undertake several complex operations,albeit with consi-derable morbidity and mortality,as is the case for the management of complicated acute pancreatitis or chronic pancreatitis.The centralisation of pancreatic surgery services,with the development of designated large-volume centres,has contribu-ted to significantly improved outcomes.In this editorial,we discuss the complex associations between diabetes mellitus(DM)and pancreatic/periampullary disease in the context of pancreatic surgery and overall management of complex pancreatitis,highlighting the consequential needs and the indispensable role of specialist diabetes teams in support of tertiary pancreatic services.Type 3c pan-creatogenic DM,refers to DM developing in the setting of exocrine pancreatic disease,and its identification and management can be challenging,while the glycaemic control of such patients may affect their course of treatment and outcome.Adequate preoperative diabetes assessment is warranted to aid identification of patients who are likely to need commencement or escalation of glucose lowering therapy in the postoperative period.The incidence of new onset diabetes after pancreatic resection is widely variable in the literature,and depends on the type and extent of pancreatic resection,as is the case with pancreatic parenchymal loss in the context of severe pancreatitis.Early involvement of a specialist diabetes team is essential to ensure a holistic management.In the current era,large volume pancreatic surgery services commonly abide by the principles of enhanced recovery after surgery,with inclusion of provisions for optimisation of the perioperative glycaemic control,to improve outcomes.While various guidelines are available to aid perioperative management of DM,auditing and quality improvement platforms have highlighted deficiencies in the perioperative management of diabetic patients and areas of required improvement.The need for perioperative support of diabetic patients by specialist diabetes teams is uniformly underlined,a fact that becomes clearly more prominent at all different stages in the setting of pancreatic surgery and the management of complex pancreatitis.Therefore,pancreatic surgery and tertiary pancreatitis services must be designed with a provision for support from specialist diabetes teams.With the ongoing accumulation of evidence,it would be reasonable to consider the design of specific guidelines for the glycaemic management of these patients.

Ziwan-Taoren herb pair can exert an therapeutical effect in primary Sjogren’s syndrome through inhibiting the TLR/NF-κB pathway

Background:Ziwan and Taoren(ZT)is a classic medicine pair in the formula of Mai Dong Di Shao Decoction,has been used to treat primary Sjogren’s syndrome(pSS)for more than 20 years.But its action mechanism is still unknown.This study is aimed to reveal the potential mechanism of ZT treated pSS and discover its active compounds of ZT and therapeutic target for pSS.Methods:Firstly,the potential pathways of ZT for pSS treatment were predicted through network pharmacology and GO and KEGG enrichment analysis.Secondly,the inter-structural relationships between active compounds of ZT and target proteins were visualized using molecular docking techniques.Finally,efficacy and mechanism were conducted through in vivo experiments,such as water intake,spleen index,hematoxylin-eosin staining pathological changes,ELISA,Western Blot analysis,and immunofluorescence staining.Results:Nine active compounds were extracted from network pharmacology,including quercitrin,luteolin,kaempferol,β-sitosterol,isorhamnetin,galangin,hederagenin,diosmetin and gibberellin 7.Seven disease targets were identified:RELA,TP53,AKT1,interleukin(IL)6,MAPK1,ESR1,IL10;with RELA being the most core target.KEGG and GO enrichment analysis indicated that ZT may act through the TLR/NF-κB/RELA inflammatory mechanism process.preliminary results of molecular docking showed that ZT’s active compounds bind well to the RELA(p65)receptor.In vivo results demonstrated that a high dose of ZT significantly improved water intake and reduced lymphocytes infiltration in submandibular gland pathology in NOD mice.The expression content of AQP5 and vasoactive intestinal peptide in the submaxillary gland was significantly increased,while levels of inflammatory factors such as tumor necrosis factor-α,IL-6,and IL-1βalong with protein expressions including toll-like receptor4,p-p65 and p-IKKα/βin NF-κB pathway were reduced.Conclusions:The ZT treatment exhibits a promising efficacy in mitigating dryness symptoms of pSS,potentially attributed to its capacity for suppressing the TLR/NF-κB inflammatory signaling pathway.

S100b联合CRP在诊断早产儿脑损伤中的临床价值

目的探讨S100b联合CRP在诊断早产儿脑损伤(Brain injury in premature infants,BIPI)中的临床效能。方法选择2022年1月—2024年1月于本院儿科确诊的125例BIPI患儿作为BIPI组,另选择同期收治的85例非BIPI早产儿作为对照组。根据BIPI患儿病情严重程度分为轻度BIPI组和重度BIPI组两组亚组。收集所有研究对象的临床资料并检测血清中S100b联合CRP表达水平。Logistic回归分析发生BIPI的危险因素,S100b联合CRP在诊断BIPI的临床效能采用ROC曲线分析。结果对照组、轻度BIPI组和重度BIPI组三组间S100b、CRP表达水平存在统计学差异(P<0.05)。与对照组相比,轻度和重度BIPI组S100b、CRP表达水平均明显升高(P<0.05);与轻度BIPI组相比,重度BIPI组S100b、CRP表达水平均明显升高(P<0.05)。与对照组相比,宫内窘迫、出生后窒息、有创机械通气、新生儿呼吸窘迫综合征发生率及血清中S100b、CRP表达水平在BIPI组中明显升高(P<0.05)。Logistic回归分析结果显示,有创机械通气(OR=1.432)、新生儿呼吸窘迫综合征(OR=2.543)、S100b(OR=3.782)和CRP表达(OR=3.449)是发生BIPI的危险因素。S100b及CRP在诊断BIPI的曲线下面积分别为0.895和0.812,S100b联合CRP在诊断BIPI的曲线下面积为0.931,诊断敏感度和特异度分别为97.43%和92.25%。结论BIPI患儿外周血中S100b和CRP表达水平显著升高,S100b联合CRP在诊断BIPI中具有显著的临床价值。

治疗冷凝素病的新药C1s补体抑制剂—Sutimlimab

冷凝集素病(Cold agglutinin disease,CAD)是一种临床少见的由补体经典途径介导的一种明确的、克隆性的低级别的骨髓淋巴增生性疾病,主要以自身免疫性溶血性贫血(Autoimmune hemolytic anemia,AIHA)为特征[1],通常由免疫球蛋白M抗体(冷凝集素)与红细胞结合并固定补体引起。目前,CAD无明确的统一治疗方案,主要包括药物治疗和非药物治疗。临床常用的药物中,传统类固醇药仅在高不可接受的剂量下有效;B细胞靶向药物主要包括利妥昔单抗,其单药或是联合苯达莫司汀或氟达拉滨或硼替佐米治疗,具有一定的疗效。虽然利妥昔单抗与细胞抑制剂的组合增加了反应率和反应持续时间,但利妥昔单抗不但会消耗正常B细胞,导致机体免疫功能低下,且有在一年内疾病复发的高风险,并常伴随着明显药物毒性的不良反应[2],用药过程中需要及时监测其药物不良反应,以及时调整治疗剂量,尤其当治疗过程中出现对病人弊大于利的情况时,需及时停用。因此,其临床应用也具有一定的局限性。此外,目前已上市的C5抑制剂依库珠单抗,也是一种可选择的CAD药物[3-4],但经其治疗后血红蛋白上升的水平有限。非药物治疗措施主要包括输血、血浆置换、脾切除及注意保暖等,临床疗效也不尽人意,尤其是反复输血有异体免疫或铁超载的风险。因此,开发新的治疗CAD的药物是临床所迫切需要的。

Gamma-glutamyl transferase 5 overexpression in cerebrovascular endothelial cells improves brain pathology,cognition,and behavior in APP/PS1 mice

In patients with Alzheimer’s disease,gamma-glutamyl transferase 5(GGT5)expression has been observed to be downregulated in cerebrovascular endothelial cells.However,the functional role of GGT5 in the development of Alzheimer’s disease remains unclear.This study aimed to explore the effect of GGT5 on cognitive function and brain pathology in an APP/PS1 mouse model of Alzheimer’s disease,as well as the underlying mechanism.We observed a significant reduction in GGT5 expression in two in vitro models of Alzheimer’s disease(Aβ_(1-42)-treated hCMEC/D3 and bEnd.3 cells),as well as in the APP/PS1 mouse model.Additionally,injection of APP/PS1 mice with an adeno-associated virus encoding GGT5 enhanced hippocampal synaptic plasticity and mitigated cognitive deficits.Interestingly,increasing GGT5 expression in cerebrovascular endothelial cells reduced levels of both soluble and insoluble amyloid-βin the brains of APP/PS1 mice.This effect may be attributable to inhibition of the expression ofβ-site APP cleaving enzyme 1,which is mediated by nuclear factor-kappa B.Our findings demonstrate that GGT5 expression in cerebrovascular endothelial cells is inversely associated with Alzheimer’s disease pathogenesis,and that GGT5 upregulation mitigates cognitive deficits in APP/PS1 mice.These findings suggest that GGT5 expression in cerebrovascular endothelial cells is a potential therapeutic target and biomarker for Alzheimer’s disease.

中重度颅脑创伤患者血清STC1 S100B和NETRIN-1的表达及对预后的预测价值

目的探讨斯钙素抗体-1(STC1)、S100钙结合蛋白B(S100B)、轴突生长诱向因子-1(NETRIN-1)在中重度创伤性颅脑损伤(TBI)术后患者血清中的表达水平及预测预后的价值。方法收集74例TBI患者及40名健康者的血清样本,使用q RT-PCR检测健康者与TBI患者术前及术后第1天、第3天、第5天、第7天的血清STC1、S100B、NETRIN-1表达水平。收集TBI患者的一般临床资料,使用扩展版格拉斯哥结局量表(EGOS)评估患者预后,根据EGOS评分将患者分为预后良好组(EGOS≥5分,n=40)及预后不良组(EGOS<5分,n=34)。分析TBI患者术后血清STC1、S100B、NETRIN-1表达水平与预后的关系。采用受试者工作特征(ROC)曲线分析STC1、S100B、NETRIN-1表达水平对患者预后的预测价值,多因素Logistic分析评估引起TBI患者预后不良的因素。结果q RT-PCR分析显示,与健康者相比,TBI患者术前血清STC1、S100B水平升高,NETRIN-1水平降低,术后血清STC1、S100B水平降低,NETRIN-1水平升高(P<0.05)。TBI患者术后第1、3、5、7天血清STC1、S100B水平逐渐降低,NETRIN-1水平逐渐升高,术后第5天最显著(P<0.05)。与术后第5天预后良好组患者相比,预后不良组患者血清STC1、S100B水平显著升高,NETRIN-1水平显著降低(P<0.05)。皮尔逊相关性分析显示术后第5天TBI患者血清STC1、S100B水平与APACHEⅡ评分呈正比,NETRIN-1水平与APACHEⅡ评分呈反比(P<0.05)。ROC曲线分析发现术后第5天血清STC1、S100B、NETRIN-1水平对TBI患者预后具有预测价值,多因素Logistic回归分析显示仅STC1水平与患者预后相关(P<0.05)。结论STC1、S100B、NETRIN-1在TBI患者血清中表达水平异常,对TBI患者预后分析具有较高价值。

Bruton’s tyrosine kinase inhibitors in primary central nervous system lymphoma:New hopes on the horizon

In this editorial,we comment on the article by Wang et al.This manuscript explores the potential synergistic effects of combining zanubrutinib,a novel oral inhibitor of Bruton’s tyrosine kinase,with high-dose methotrexate(HD-MTX)as a therapeutic intervention for primary central nervous system lymphoma(PCNSL).The study involves a retrospective analysis of 19 PCNSL patients,highlighting clinicopathological characteristics,treatment outcomes,and genomic biomarkers.The results indicate the combination’s good tolerance and strong antitumor activity,with an 84.2%overall response rate.The authors emphasize the potential of zanubrutinib to modulate key genomic features of PCNSL,particularly mutations in myeloid differentiation primary response 88 and cluster of differentiation 79B.Furthermore,the study investigates the role of circulating tumor DNA in cerebrospinal fluid for disease surveillance and treatment response monitoring.In essence,the study provides valuable insights into the potential of combining zanubrutinib with HD-MTX as a frontline therapeutic regimen for PCNSL.The findings underscore the importance of exploring alternative treatment modalities and monitoring genomic and liquid biopsy markers to optimize patient outcomes.While the findings suggest promise,the study’s limitations should be considered,and further research is needed to establish the clinical relevance of this therapeutic approach for PCNSL.

妊娠糖尿病患者蛋白C、蛋白S和抗凝血酶Ⅲ水平及其与孕周的关系

目的探讨妊娠糖尿病(GDM)患者蛋白C(PC)、蛋白S(PS)和抗凝血酶Ⅲ(ATⅢ)水平及其与孕周的关系。方法选择2019年1月至2023年3月在医院就诊的232例GDM孕妇作为观察组,同时随机选取同期在医院进行孕检的268例正常孕妇作为对照组,比较两组一般资料和PC、PS和ATⅢ水平;按孕周将观察组和对照组分为孕中期和孕晚期,分别比较孕中期和孕晚期观察组和对照组PC、PS和ATⅢ水平。通过相关性分析,分析PC、PS和ATⅢ水平与孕周的相关性。结果与对照组相比,观察组PS和PC水平降低(P<0.05),但ATⅢ水平差异无统计学意义(P>0.05)。GDM患者PC和ATⅢ水平与孕周呈负相关(r=-0.156、-0.134,P<0.05)。结论GDM患者PS和PC水平降低,且随孕周增大,PC、PS和ATⅢ水平降低,应尽早进行干预,以减少血栓形成的风险。

基于B/S架构的远程会诊质控系统的设计与应用

目的研发远程会诊质控系统,在区域内实现远程会诊质控、管理、监管、考评、统计和持续改进的信息化。方法2020年,组建多学科团队,运用智力激励法和专题小组讨论法,基于B/S架构研发远程会诊质控系统,与既有远程会诊信息系统完全嵌合兼容,满足远程会诊全流程质量管理的功能需求。结果针对远程会诊质控工作要求,系统设计研发了质控管理、质控考评、实时监管、统计分析4个子模块,可满足远程会诊全流程质量管理的功能需求。结论该质控系统创新了远程会诊信息化质量管理模式,在区域内实现了远程会诊全流程的信息化管理,取得了较好的质量管理成效,有效促进区域内远程会诊工作的推进和发展。

基于B/S和SOA架构的LIMS系统设计与研究

为提升水质检测业务的专业化、智能化、系统化、自动化水平,针对新发展阶段下水利高质量发展对水质信息管理的需求,研究并开发基于B/S系统架构和SOA设计架构的新型综合性水质信息管理系统(LIMS),运用仪器谱图识别及数据提取、PHP与MySQL融合、仪器谱图与原始记录自动关联等技术,在一定程度上提高水质数据的电子化无纸化获取、处理、传输及应用等方面的管理水平,实现水质检测业务流程与资源的动态关联和闭环追溯,提升水质监测评价工作的管理效率和质量管理能力。基于B/S和SOA架构的LIMS在前沿高新技术中的应用研究,为水质检测和管理的各项活动提供智能、高效的数据支持平台,也为水利行业信息系统管理建设及水域功能监测监管提供技术支撑。

NOTCH3基因C260S位点突变导致CADASIL的临床和影像学特征分析

目的探讨NOTCH3基因第5外显子C260S位点突变导致的伴有皮层下梗死和白质脑病的常染色体显性遗传性脑动脉病(cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy,CADASIL)家系的临床和影像学特征。方法选取2021年12月首都医科大学附属北京同仁医院来自同一家庭的CADASIL患者,对所有患者进行NOTCH3基因测序,回顾性分析患者的临床表现和头颅影像学特征。复习既往文献报道的导致同一位置氨基酸改变的其他突变类型的临床及影像学特征。结果4名家庭成员中,包括先证者(46岁,女)及其两个姐姐(分别为48岁和50岁)和女儿(18岁)。先证者及其父亲、两个姐姐都有偏头痛病史,其中大姐有记忆力减退;先证者患有脑梗死及伴有视觉先兆的偏头痛;先证者女儿体健;先证者父亲因脑梗死去世。4名家庭成员均存在C260S位点的NOTCH3基因突变。既往文献无此位点突变的报道,先证者头颅MRI示右侧脑桥亚急性梗死,颞叶、脑室周围及脑干异常高信号改变,其大姐脑桥可见腔隙性梗死灶。结论NOTCH3基因第5外显子c.778T>A(p.C260S)的罕见突变导致的CADASIL发病时间早,早期会出现认知障碍。合并偏头痛的脑干梗死患者,需警惕CADASIL的可能。

基于C/S架构和物联网技术的可移动医疗设备共享调配系统的设计与应用

目的 设计一个可移动医疗设备高效管理与临床科室共享调配相结合的医疗设备共享管理平台,实现可移动医疗设备使用的全过程管理。方法 基于C/S架构,利用物联网技术优化整合现有医疗设备大数据资源,结合医院需求,构建可移动医疗设备共享调配系统,实现医疗设备共享管理、预约借用、统计分析等核心功能。通过比较系统上线前后呼吸机调配次数、平均调配时间、呼吸机使用率、单台呼吸机日均收入4个指标,分析系统应用效果。结果 系统上线后,呼吸机调配次数明显增加、平均调配时间明显降低,呼吸机使用率、单台呼吸机日均收入明显升高,且差异均有统计学意义(P﹤0.05),表明系统对提高呼吸机调配效率与使用效率有促进作用。结论 可移动医疗设备共享调配系统优化了医院设备管理流程,实现了医疗资源的统一调配,有效满足临床紧急抢救时医疗设备调配使用的相关需求,提高了医疗设备周转率以及设备管理人员的工作效率,有助于医院节约人员成本、降低采购费用,为医院管理者制定相关决策提供数据支撑。

基于Spring Boot的产教联合体信息平台设计

该文设计基于B/S架构的联合体信息平台,利用Spring Boot、MyBatis Plus、Vue等优秀框架实现高效的开发,集成Spring Security和JWT令牌机制,完成认证和权限控制。同时整合Redis缓存,将热点资源缓存至内存中,以实现快速查询操作,从而减轻MySQL数据库的负载压力。该系统稳定、兼容性强,能使联合体成员沟通更方便、信息的发布和维护的效率更高,实现人才共育共享。

SLC35E基因家族在麻风皮损中的表达

目的:明确溶质转运体家族蛋白SLC35E在多菌型(MB)与少菌型(PB)麻风皮损中的差异。方法:首先收集25例确诊为麻风感染患者的石蜡包埋皮损组织,其中多菌型麻风患者12例,少菌型13例,分析SLC35E1和SLC35E2B不同SNP位点的突变情况;同时收集12名健康对照者皮肤组织作为正常对照组,采用免疫组化染色法检测SLC35E1、SLC35E2B、CD68、S100在上述皮损及正常对照组中的表达差异,免疫荧光染色法检测CD68、S100与SLC35E1、SLC35E2B蛋白在皮损组织中的共表达情况。结果:对25例麻风组织样本进行生物信息学分析,SLC35E1的SNP位点rs202071873在麻风患者中的突变率为0%,SLC35E2B的3个SNP位点rs12729295、rs117820608、rs2072923在麻风患者中的突变率分别为100%、40%,100%。免疫组化染色显示SLC35E1、SLC35E2B、CD68、S100在麻风组织的真皮及表皮均有表达,其中CD68主要表达于麻风组织真皮,SLC35E1在真皮层的表达量与CD68基本一致,两者表达量基本呈正相关;S100在麻风患者表皮和真皮中的表达无显著差异,与SLC35E1、SLC35E2B表达量无明显相关性;与少菌型麻风相比,多菌型麻风患者SLC35E1在真皮层呈低表达(P=0.046);与健康对照组相比,多菌型麻风患者SLC35E2B在表皮层呈低表达(p=0.004);免疫荧光共染显示麻风组织皮损中CD68与SLC35E1、SLC35E2B蛋白共染均出现重叠结果,且与SLC35E1重叠荧光强度高于SLC35E2B;S100与SLC35E1、SLC35E2B蛋白共染未见重叠结果。结论:麻风患者皮损中可能存在SLC35E2B位点突变,SLC35E1的表达可预测多菌型(MB)与少菌型(PB)麻风在真皮层感染差异,SLC35E1、SLC35E2B可能通过影响巨噬细胞功能干扰机体对麻风分枝杆菌的清除,进而影响麻风的发病。

基于B/S的高校实验室预约管理系统设计与实现

为了实现实验室管理信息化,更好地为师生服务,文章设计并开发一套高校实验室预约管理系统。该系统是基于B/S结构,采用Vue视图框架、Nginx服务器及MySQL数据库构建而成的;支持专业实验室、教室和会议室的预约管理;入口对接华中科技大学统一身份认证系统,在校师生可直接使用已有的统一身份认证系统账号密码登录系统。用户可通过浏览器完成在线预约、预约查询、预约管理和数据统计等功能。该系统的应用提高了实验室管理的规范性和信息化程度。

基于B/S结构的排课系统数据库设计

排课系统是高校教务管理中的重要部分,随着学生规模的扩大和课程的日新月异,如何高效解决学生的学习需求和教室及教师之间的冲突成为重难点。传统的Excel管理方式已无法满足需求,因此需要引入数据库管理排课信息。文章主要介绍排课系统的数据库设计,包括需求分析、逻辑结构设计、实体属性定义和信息表设计等。通过引入浏览器/服务器模式(Browser/Server,B/S)架构和关系型数据库,教务管理人员、学生和教师随时随地进行课程排列与查询,提高工作和学习效率。同时,数据库性能设计和安全性设计也是该系统的重点考虑因素。