Flexible skew thin plate is widely used in mechanical engineering,architectural engineering and structural engineering.High-precision analysis is very important for structural design and improvement.In this paper,the ...Flexible skew thin plate is widely used in mechanical engineering,architectural engineering and structural engineering.High-precision analysis is very important for structural design and improvement.In this paper,the multivariable wavelet finite element(MWFE)based on B-spline wavelet on the interval(BSWI)is constructed for flexible skew thin plate analysis.First,the finite element formulation is derived from multivariable generalized potential energy function.Then the generalized field variables are interpolated and calculated by BSWI.Different from the traditional wavelet finite element,the analysis precision can be improved because the generalized displacement and stress field variables are interpolated and calculated independently,the secondary calculation and the computational error are avoided.In order to verify the effectiveness of the constructed MWFE,several numerical examples are given in the end.展开更多
The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineur...The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.51225501)the Fundamental Research Funds for the Central Universities+2 种基金the Project funded by China Postdoctoral Science Foundation(Grant No.2014M552432)the National Science and Technology Major Project of China(Grant No.2012ZX04002071)the Program for Changjiang Scholars and Innovative Research Team in University
文摘Flexible skew thin plate is widely used in mechanical engineering,architectural engineering and structural engineering.High-precision analysis is very important for structural design and improvement.In this paper,the multivariable wavelet finite element(MWFE)based on B-spline wavelet on the interval(BSWI)is constructed for flexible skew thin plate analysis.First,the finite element formulation is derived from multivariable generalized potential energy function.Then the generalized field variables are interpolated and calculated by BSWI.Different from the traditional wavelet finite element,the analysis precision can be improved because the generalized displacement and stress field variables are interpolated and calculated independently,the secondary calculation and the computational error are avoided.In order to verify the effectiveness of the constructed MWFE,several numerical examples are given in the end.
基金supported by the National Important Novel Medicine Research Project (2012ZX09304010, 2013ZX09102062)the National Natural Science Foundation of China (31270849)
文摘The aim of this study was to establish a quality-control method for calcineurin subunit B(CNB) biological activity determinations. CNB enhances the p-nitrophenylphosphate(p NPP) dephosphorylating activity of calcineurin subunit A Δ316 mutant(CNAΔ316). A series of CNB concentrations were fitted to a four-parameter equation to calculate the corresponding p NPP maximum dephosphorylation rates. Values were calculated based on biological activity references using a parallel line method. The method was then validated for accuracy, precision, linearity, linear range, sensitivity, specificity, and robustness. The recovery results were greater than 98%. Intra-plate precision was 6.7%, with inter-plate precision of 10.8%. The coefficient of determination was greater than 0.98. The linear range was 0.05–50 μg m L?1, with sensitivity of 50 μg m L?1. Tested cytokines did not induce CNAΔ316 dephosphorylation of p NPP. The chosen CNAΔ316 concentration range did not affect activity determinations.
