Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two intro...Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.展开更多
文摘Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum tenellum.OPK1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 aa.Phylogenetic analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in fungi.Southern blot analysis indicated that OPK1 is present as a single copy in the genome.RT-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.
