慢性B淋巴细胞增殖性疾病根据免疫表型可分为不同亚型,免疫表型在各亚型的诊断及鉴别诊断中发挥着重要作用,典型(慢性)B淋巴细胞白血病免疫表型为CD5+、CD23+、FMC(flinders medical centre)7+;而毛细胞白血病特异性高表达CD22、CD25、C...慢性B淋巴细胞增殖性疾病根据免疫表型可分为不同亚型,免疫表型在各亚型的诊断及鉴别诊断中发挥着重要作用,典型(慢性)B淋巴细胞白血病免疫表型为CD5+、CD23+、FMC(flinders medical centre)7+;而毛细胞白血病特异性高表达CD22、CD25、CD103和CD11c;滤泡性淋巴瘤主要表达CD20、CD10、CD22、CD79a,不表达CD5;套细胞淋巴瘤则过度表达CD5+、FMC7+、Bcl-2+、细胞周期蛋白(cyclinD1+);典型瓦氏巨球蛋白血症不表达CD5及CD23,而变异型则表达;边缘区B细胞淋巴瘤的典型免疫表型为CD5-、CD10-、CD23-、cyclinD1-。对于不典型的慢性B淋巴细胞增殖性疾病需结合病理学、遗传学、分子生物学诊断,从而指导临床治疗及预后。展开更多
Objective To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parame...Objective To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parameters. Methods Quantities of CD5+ B lymphocytes in the bone marrow of 14 patients with autoimmune hemolytic anemia (AIHA) or Evans syndrome, 22 immunorelated pancytopenia (IRP) patients, and 10 normal controls were assayed by flow cytometry. The correlation between their clinical or laboratorial parameters and CD5+ B lymphocytes was analyzed. Results The quantity of CD5+ B lymphocytes of AIHA/Evans syndrome (34.64%±19.81%) or IRP patients (35.81%±16.83%) was significantly higher than that of normal controls (12.00%±1.97%, P<0.05). However, there was no significant difference between AIHA/Evans syndrome and IRP patients (P>0.05). In all hemocytopenic patients, the quantity of bone marrow CD5+ B lymphocytes showed significantly negative correlation with serum complement C3 level (r=-0.416, P<0.05). In the patients with AIHA/Evans syndrome, the quantity of bone marrow CD5+ B lymphocytes showed significantly positive correlation with serum indirect bilirubin level (r=1.00, P<0.05). In Evans syndrome patients, the quantity of CD5+ B lymphocytes in bone marrow showed significantly positive correlation with platelet-associated immunoglobulin G (r=0.761, P<0.05) and platelet-associated immunoglobulin M (r=0.925, P<0.05). The quantity of CD5+ B lymphocytes in bone marrow of all hemocytopenic patients showed significantly negative correlation with treatment response (tau-b=-0.289, P<0.05), but had no correlation with colony forming unit-erythroid (r=-0.205, P>0.05) or colony forming unit-granulocyte-macrophage colonies (r=-0.214, P>0.05). Conclusions The quantity of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia significantly increases and is correlated with disease severity and clinical response, which suggest that CD5+ B lymphocytes might play an important role in the pathogenesis of autoimmune hemocytopenia.展开更多
文摘慢性B淋巴细胞增殖性疾病根据免疫表型可分为不同亚型,免疫表型在各亚型的诊断及鉴别诊断中发挥着重要作用,典型(慢性)B淋巴细胞白血病免疫表型为CD5+、CD23+、FMC(flinders medical centre)7+;而毛细胞白血病特异性高表达CD22、CD25、CD103和CD11c;滤泡性淋巴瘤主要表达CD20、CD10、CD22、CD79a,不表达CD5;套细胞淋巴瘤则过度表达CD5+、FMC7+、Bcl-2+、细胞周期蛋白(cyclinD1+);典型瓦氏巨球蛋白血症不表达CD5及CD23,而变异型则表达;边缘区B细胞淋巴瘤的典型免疫表型为CD5-、CD10-、CD23-、cyclinD1-。对于不典型的慢性B淋巴细胞增殖性疾病需结合病理学、遗传学、分子生物学诊断,从而指导临床治疗及预后。
文摘Objective To investigate the quantities of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia and the relationship between quantities of CD5+ B lymphocytes and clinical or laboratorial parameters. Methods Quantities of CD5+ B lymphocytes in the bone marrow of 14 patients with autoimmune hemolytic anemia (AIHA) or Evans syndrome, 22 immunorelated pancytopenia (IRP) patients, and 10 normal controls were assayed by flow cytometry. The correlation between their clinical or laboratorial parameters and CD5+ B lymphocytes was analyzed. Results The quantity of CD5+ B lymphocytes of AIHA/Evans syndrome (34.64%±19.81%) or IRP patients (35.81%±16.83%) was significantly higher than that of normal controls (12.00%±1.97%, P<0.05). However, there was no significant difference between AIHA/Evans syndrome and IRP patients (P>0.05). In all hemocytopenic patients, the quantity of bone marrow CD5+ B lymphocytes showed significantly negative correlation with serum complement C3 level (r=-0.416, P<0.05). In the patients with AIHA/Evans syndrome, the quantity of bone marrow CD5+ B lymphocytes showed significantly positive correlation with serum indirect bilirubin level (r=1.00, P<0.05). In Evans syndrome patients, the quantity of CD5+ B lymphocytes in bone marrow showed significantly positive correlation with platelet-associated immunoglobulin G (r=0.761, P<0.05) and platelet-associated immunoglobulin M (r=0.925, P<0.05). The quantity of CD5+ B lymphocytes in bone marrow of all hemocytopenic patients showed significantly negative correlation with treatment response (tau-b=-0.289, P<0.05), but had no correlation with colony forming unit-erythroid (r=-0.205, P>0.05) or colony forming unit-granulocyte-macrophage colonies (r=-0.214, P>0.05). Conclusions The quantity of bone marrow CD5+ B lymphocytes in the patients with autoimmune hemocytopenia significantly increases and is correlated with disease severity and clinical response, which suggest that CD5+ B lymphocytes might play an important role in the pathogenesis of autoimmune hemocytopenia.
