肝脏疾病是一类严重的公共卫生问题，很多原因均可导致肝脏发生病变，长期肝损伤将最终发展为肝衰竭［1］。伴刀豆球蛋白 A （concanavalin A， Con A）诱导的急性肝损伤模型是一个较理想的 T细胞介导的肝炎小鼠模型，常用来模拟人病毒...肝脏疾病是一类严重的公共卫生问题，很多原因均可导致肝脏发生病变，长期肝损伤将最终发展为肝衰竭［1］。伴刀豆球蛋白 A （concanavalin A， Con A）诱导的急性肝损伤模型是一个较理想的 T细胞介导的肝炎小鼠模型，常用来模拟人病毒性和自身免疫性肝病［2］。本课题组早期利用弱阳离子磁珠纯化和基质辅助激光解析离子化飞行时间质谱分析寻找肝硬化和肝癌潜在的早期血清生物学标志物的过程中，分离得到一个相对分子质量为4210000的多肽［3］，进一步分析显示该多肽为真核肽链释放因子3b （eukaryotic peptiae chain release factor GTP-bind subunit，eRF3b）的一个36个氨基酸片段，其在慢性肝炎患者中表达水平高于肝硬化和肝癌患者。本研究观察人工合成的该 eRF3b 片段对Con A 诱导的小鼠急性肝损伤的预防及治疗作用，旨在为临床治疗肝病提供实验依据。展开更多
[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mit...[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.[Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.[Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab.展开更多
基金Supported by Application Basic Research Foundation of TianjinInternational Cooperation Fund(033803511,033803511G)the Tianjin Higher Education Science and Technology Development Fund(2004BA31)~~
文摘[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis（DGGE）.[Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.[Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.[Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab.
