In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B...In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.展开更多
Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of...Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of melanogenesis-related genes and proteins were analyzed inα-melanocyte-stimulating hormone(α-MSH)-stimulated B16F10 cells.Results:α-MSH treatment significantly increased tyrosinase activity,and extracellular and intracellular melanin content,as well as the expression levels of tyrosinase,microphthalmia-associated transcription factor(MITF),tyrosinase-related protein(TRP)-1,and TRP-2 in B16F10 cells.Treatment with Cyrtomium falcatum crude extract and its solvent fractions reduced tyrosinase activity and extracellular and intracellular melanin content and downregulated the expression levels of tyrosinase,MITF,TRP-1,and TRP-2 in a dose-dependent manner.Conclusions:Cyrtomium falcatum has potential anti-melanogenesis effects and can be used as a potential source material in cosmeceutical industry for the research and development of novel lead molecules with whitening properties.展开更多
Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells....Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells. Biomolecular analyses and confocal microscopy indicated excessive expansion followed by contraction contributed to the “explosion” of the cell. However, after months of replicable effects, the phenomenon slowly ceased. Considering the potency of the complete dissolution of cancer cell lines after 5 days of 6.5-hour daily exposures and the implications for human treatment, the potential source of the disappearance of the effect was pursued by summarizing all of the 50 experiments and assessing the likely etiologies. Materials and Methods: B16-BL6, MDAMB 231 and MCF7 malignant cells and HSG, a non-malignant cell line, were exposed to a sham-field condition or to a specific pattern of computer-generated magnetic fields produced from converting different voltages, each with point durations of 3 ms to 3-D magnetic fields. Conclusion: The specific serial presentation of the two field patterns (one frequency modulated;the other amplitude and frequency modulated) completely dissolved malignant cells but not normal cells within a “zone” within the exposure volume at the conjunction of the three planes of the applied magnetic fields. The affected cells underwent massive melanin production, expansion, contraction and “beading” of submembrane actin structures before fragmentation within this zone. However, this powerful all-or-none phenomenon may have been disrupted by moving the cells, excess mechanical agitation during exposure, or non-optimal point durations of the field parameters. Indirect effects from communication signals (WIFI) through line currents that operated the incubators could not be excluded.展开更多
Aim:We aimed to test the hypothesis that loading of dendritic cells(DCs)with both viral and tumor-specific antigens would enhance the efficacy antitumor DC-based therapy applied simultaneously with oncolytic virus.Met...Aim:We aimed to test the hypothesis that loading of dendritic cells(DCs)with both viral and tumor-specific antigens would enhance the efficacy antitumor DC-based therapy applied simultaneously with oncolytic virus.Methods:Vaccinia virus LIVP/GFP and melanoma B16-F10 were used in this study.DCs were pulsed with various combinations of viral and tumor-associated antigens.The maturation status of DCs was verified by expression of the markers CD80,CD86,and CCR7 and assessment of IL-6,TNF-α,and IL-12 secretion.The most efficient combination of antigens for DC loading was selected based on the analysis of the cytotoxic activity of T lymphocytes.Combination therapy using vaccinia virus LIVP/GFP and DCs pulsed with viral and tumor-specific antigens was administered to the B16-F10 melanoma/mouse C57Bl tumor model.Results:We found that loading of DCs with viral antigens,or with a combination of viral and tumor antigens,resulted in similar levels of expression of DC maturation markers.The maximal in vitro cytotoxicity against virus-infected and non-infected B16 melanoma cells exhibited T lymphocytes activated by DCs loaded with the heat inactivated lysate of vaccinia virus LIVP/GFP infected tumor cell.The results show that the combination of vaccinia virus LIVP/GFP and DCs loaded with both tumor and viral antigens inhibit tumor growth of B16-F10 murine melanoma by more than two-fold.Conclusions:Combination therapy with oncolytic vaccinia virus LIVP/GFP and tumor/virus antigen-loaded DCs limited the growth of established melanoma B16-F10,but no synergistic antitumor effects were observed.We propose that optimization of the therapy regimen could enhance the efficiency of combination therapy.展开更多
文摘In this study, we aimed to use a novel approach to overcome the current limitations of ozone therapy in medicine through ozonized oil nanoemulsions (OZNEs). We evaluated dose-dependency on the cellular activities of B-16 melanoma cell line which were incubated with various OZNE doses (v/v). Antitumor effects of OZNE against cancer cell lines were evaluated by cellular morphology, apoptosis and cell cycle analysis. Flow cytometry results showed that OZNE induced DNA damage, apoptosis, and arrested cell cycle in G0-1 phase in B-16 melanoma cells. Thus, OZNE treatment could pose an effective way to act as a potential therapeutic for patients with tumors in the future.
基金This work was supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.2023R1A2C1006268 and RS-2023-00212560).
文摘Objective:To explore the anti-melanogenic potential of Cyrtomium falcatum.Methods:The effects of Cyrtomium falcatum crude extract and its solvent fractions on tyrosinase activity,melanin content,and the expressions of melanogenesis-related genes and proteins were analyzed inα-melanocyte-stimulating hormone(α-MSH)-stimulated B16F10 cells.Results:α-MSH treatment significantly increased tyrosinase activity,and extracellular and intracellular melanin content,as well as the expression levels of tyrosinase,microphthalmia-associated transcription factor(MITF),tyrosinase-related protein(TRP)-1,and TRP-2 in B16F10 cells.Treatment with Cyrtomium falcatum crude extract and its solvent fractions reduced tyrosinase activity and extracellular and intracellular melanin content and downregulated the expression levels of tyrosinase,MITF,TRP-1,and TRP-2 in a dose-dependent manner.Conclusions:Cyrtomium falcatum has potential anti-melanogenesis effects and can be used as a potential source material in cosmeceutical industry for the research and development of novel lead molecules with whitening properties.
文摘Purpose: Exposure to a particular pattern of weak (~3 to 5 μT) magnetic fields produced by computer-generated point durations within three-dimensions completely dissolved malignant cancer cells but not healthy cells. Biomolecular analyses and confocal microscopy indicated excessive expansion followed by contraction contributed to the “explosion” of the cell. However, after months of replicable effects, the phenomenon slowly ceased. Considering the potency of the complete dissolution of cancer cell lines after 5 days of 6.5-hour daily exposures and the implications for human treatment, the potential source of the disappearance of the effect was pursued by summarizing all of the 50 experiments and assessing the likely etiologies. Materials and Methods: B16-BL6, MDAMB 231 and MCF7 malignant cells and HSG, a non-malignant cell line, were exposed to a sham-field condition or to a specific pattern of computer-generated magnetic fields produced from converting different voltages, each with point durations of 3 ms to 3-D magnetic fields. Conclusion: The specific serial presentation of the two field patterns (one frequency modulated;the other amplitude and frequency modulated) completely dissolved malignant cells but not normal cells within a “zone” within the exposure volume at the conjunction of the three planes of the applied magnetic fields. The affected cells underwent massive melanin production, expansion, contraction and “beading” of submembrane actin structures before fragmentation within this zone. However, this powerful all-or-none phenomenon may have been disrupted by moving the cells, excess mechanical agitation during exposure, or non-optimal point durations of the field parameters. Indirect effects from communication signals (WIFI) through line currents that operated the incubators could not be excluded.
基金This work was supported by the Russian Foundation for Basic Research project#18-34-20109Russian state budget of ICBFM SB RAS project#121031300044-5Russian Science Foundation RSF#19-74-30011.
文摘Aim:We aimed to test the hypothesis that loading of dendritic cells(DCs)with both viral and tumor-specific antigens would enhance the efficacy antitumor DC-based therapy applied simultaneously with oncolytic virus.Methods:Vaccinia virus LIVP/GFP and melanoma B16-F10 were used in this study.DCs were pulsed with various combinations of viral and tumor-associated antigens.The maturation status of DCs was verified by expression of the markers CD80,CD86,and CCR7 and assessment of IL-6,TNF-α,and IL-12 secretion.The most efficient combination of antigens for DC loading was selected based on the analysis of the cytotoxic activity of T lymphocytes.Combination therapy using vaccinia virus LIVP/GFP and DCs pulsed with viral and tumor-specific antigens was administered to the B16-F10 melanoma/mouse C57Bl tumor model.Results:We found that loading of DCs with viral antigens,or with a combination of viral and tumor antigens,resulted in similar levels of expression of DC maturation markers.The maximal in vitro cytotoxicity against virus-infected and non-infected B16 melanoma cells exhibited T lymphocytes activated by DCs loaded with the heat inactivated lysate of vaccinia virus LIVP/GFP infected tumor cell.The results show that the combination of vaccinia virus LIVP/GFP and DCs loaded with both tumor and viral antigens inhibit tumor growth of B16-F10 murine melanoma by more than two-fold.Conclusions:Combination therapy with oncolytic vaccinia virus LIVP/GFP and tumor/virus antigen-loaded DCs limited the growth of established melanoma B16-F10,but no synergistic antitumor effects were observed.We propose that optimization of the therapy regimen could enhance the efficiency of combination therapy.
