BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is an aggressive non-Hodgkin lymphoma that affects B lymphocytes.It can develop in the lymph nodes and can be localized or generalized.Despite DLBCL being considered pote...BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is an aggressive non-Hodgkin lymphoma that affects B lymphocytes.It can develop in the lymph nodes and can be localized or generalized.Despite DLBCL being considered potentially curable,little research has been conducted on the relationship between the body's immune response and DLBCL.AIM To study the expression and significance of T-regulatory cells(Tregs)interleukin(IL)-35,IL-10,and transforming growth factor-beta(TGF-β)in DLBCL.METHODS Data from 82 patients with DLBCL who were initially admitted to The First Affiliated Hospital of Ningbo University(Zhejiang Province,China)between January 2017 and June 2022 and treated with standard first-line regimens were reviewed.Three patients were lost to follow-up;thus,79 patients were included in the statistical analysis and then divided into three groups according to the evaluation of clinical efficacy:Incipient(new-onset and treatment-naïve),effectively treated,and relapsed-refractory.Thirty healthy individuals were included in the control group.The expression of peripheral blood T lymphocytes and their associated factors IL-35,IL-10,and TGF-βin the four groups were observed.RESULTS In contrast to the successfully treated and normal control groups,both the incipient and relapse-refractory groups exhibited greater proportions of CD4-positive(+)Tregs(P<0.05),whereas the proportion of CD8+Tregs did not differ substantially between the groups.Serum levels of IL-35 and IL-10 in the incipient and relapsed-refractory groups were higher than those in the effectively treated and normal control groups(P<0.05).There was no statistically significant distinction in the expression level of TGF-βbetween the groups(P>0.05).The correlation between IL-35 and IL-10 concentrations was significantly positive,with a correlation coefficient of 0.531(P<0.05).The correlation between IL-35 and TGF-βconcentration was significantly positive,with a correlation coefficient of 0.375(P<0.05).The correlation between IL-10 and TGF-βconcentration was significantly positive,with a correlation coefficient of 0.185(P<0.05).The expression concentrations of IL-35,IL-10 and TGF-βwere apparently and positively correlated(P<0.05).CONCLUSION Tregs IL-35,and IL-10 may be closely associated with the occurrence and development of DLBCL and the detection of related indices may be helpful in the analysis of disease prognosis.展开更多
Spurred by better understanding of disease biology,improvements in molecular diagnostics,and the development of targeted therapies,the treatment of acute myeloid leukemia(AML)has undergone significant evolution in rec...Spurred by better understanding of disease biology,improvements in molecular diagnostics,and the development of targeted therapies,the treatment of acute myeloid leukemia(AML)has undergone significant evolution in recent years.Arguably,the most exciting shift has come from the success of treatment with the B-cell lymphoma-2 inhibitor venetoclax.When given in combination with a hypomethylating agent or low dose cytarabine,venetoclax demonstrates high response rates,some of which are durable.In spite of this,relapses after venetoclax treatment are common,and much interest exists in elucidating the mechanisms of resistance to the drug.Alterations in leukemic stem cell metabolism have been identified as a possible escape route,and clinical trials focusing on targeting metabolism in AML are ongoing.This review article highlights current research regarding venetoclax treatment and resistance in AML with a focus on cellular metabolism.展开更多
目的探讨泛素特异性蛋白酶10(ubiquitin-specifi c protease 10,USP10)蛋白及其m RNA在人弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)与淋巴结反应性增生(reactive lymph node hyperplasia,RLH)中的表达及其临床意义。方...目的探讨泛素特异性蛋白酶10(ubiquitin-specifi c protease 10,USP10)蛋白及其m RNA在人弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)与淋巴结反应性增生(reactive lymph node hyperplasia,RLH)中的表达及其临床意义。方法选取70例人DLBCL及70例RLH组织,采用组织芯片和免疫组织化学染色检测USP10的蛋白表达,分析其与DLBCL分子亚型及DLBCL预后相关分子之间的关系,并采用GEO数据库分析USP10 m RNA表达水平。结果 USP10在DLBCL组织中的阳性表达率显著高于RLH组织的B淋巴细胞,在非生发中心亚型(non-germinal center B-cell-like type,nonGCB)DLBCL组织中的阳性表达率明显高于生发中心亚型(germinal center B-cell-like type,GCB)DLBCL组织;Spearman等级相关分析显示,USP10与DLBCL预后相关分子中的CD5、Bcl-2蛋白表达呈正相关,而与p53,c-Myc及Ki67蛋白表达无相关性;USP10 m RNA在DLBCL组织及RLH组织中的表达差异。结论 USP10在DLBCL组织中的表达明显增加,尤其是在预后较差的non-GCB亚型或CD5阳性或无Bcl-2阳性的DLBCL中,表明该蛋白是DLBCL患者预后不良的分子标记物之一。展开更多
AIM: To investigate the association between endogenous hydrogen sulfide (H<sub>2</sub>S) and portal hypertension as well as its effect on vascular smooth muscle cells.
AIM： To assess the significance of chromosome translo- cation t（11;18）（q21;q21）, B-cell lymphoma 10 （BCL-10） protein and He/icobacter py/ori （H. py/on） infection in gastric mucosa-associated lymphoid tissue ...AIM： To assess the significance of chromosome translo- cation t（11;18）（q21;q21）, B-cell lymphoma 10 （BCL-10） protein and He/icobacter py/ori （H. py/on） infection in gastric mucosa-associated lymphoid tissue （MALT） lymphoma in Colombia.展开更多
BACKGROUND The concurrence of acute myeloid leukemia(AML)and chronic lymphocytic leukemia(CLL)is rare.Previous reports of such cases have focused mainly on clinical diagnosis and characteristics,so the mechanism remai...BACKGROUND The concurrence of acute myeloid leukemia(AML)and chronic lymphocytic leukemia(CLL)is rare.Previous reports of such cases have focused mainly on clinical diagnosis and characteristics,so the mechanism remains unclear,and therapy options have been poorly explored.CASE SUMMARY Here,we report two cases of synchronous AML and CLL.Flow cytometry revealed two distinct abnormal cell populations(myeloblasts and lymphoid cells)according to scatter characteristics.CD5-positive B cell lymphoma with myeloid leukemia invasion was observed on lymph node biopsy.Chemotherapy regimens indicated for both AML and CLL were used in our patients,and our patients achieved complete response after chemotherapy.Next-generation sequencing of 88 genes was performed.CONCLUSION We conclude that early mutation and dysregulation at the hematopoietic stem cell stage and the accumulation of multiple rearrangements may cause the concurrence of CLL and AML.The treatment of infection and combination therapy aimed at the CLL component are significant in the management of patients with concurrent CLL and AML.展开更多
【指示性摘要】IL-6由肿瘤细胞自分泌,与肿瘤侵袭性密切相关;TNF-α在机体肿瘤免疫应答中起到了重要作用,可引起肿瘤细胞坏死,且可诱导IL-6的表达;IFN-γ具有抗病毒,抗肿瘤和免疫调控的作用;IL-10具有双向免疫调节作用,在肿瘤环境中对...【指示性摘要】IL-6由肿瘤细胞自分泌,与肿瘤侵袭性密切相关;TNF-α在机体肿瘤免疫应答中起到了重要作用,可引起肿瘤细胞坏死,且可诱导IL-6的表达;IFN-γ具有抗病毒,抗肿瘤和免疫调控的作用;IL-10具有双向免疫调节作用,在肿瘤环境中对免疫应答具有负向调节作用,同时也能发挥刺激作用。上述细胞因子在弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)的发生发展中或起到协同作用,并可能作为DLBCL诊断及预后的指标。展开更多
基金Supported by Zhejiang TCM Science and Technology Project,No.2023ZL653Zhejiang Medical Science and Technology Plan Project-Clinical Research Application Project A,No.2021KY273。
文摘BACKGROUND Diffuse large B-cell lymphoma(DLBCL)is an aggressive non-Hodgkin lymphoma that affects B lymphocytes.It can develop in the lymph nodes and can be localized or generalized.Despite DLBCL being considered potentially curable,little research has been conducted on the relationship between the body's immune response and DLBCL.AIM To study the expression and significance of T-regulatory cells(Tregs)interleukin(IL)-35,IL-10,and transforming growth factor-beta(TGF-β)in DLBCL.METHODS Data from 82 patients with DLBCL who were initially admitted to The First Affiliated Hospital of Ningbo University(Zhejiang Province,China)between January 2017 and June 2022 and treated with standard first-line regimens were reviewed.Three patients were lost to follow-up;thus,79 patients were included in the statistical analysis and then divided into three groups according to the evaluation of clinical efficacy:Incipient(new-onset and treatment-naïve),effectively treated,and relapsed-refractory.Thirty healthy individuals were included in the control group.The expression of peripheral blood T lymphocytes and their associated factors IL-35,IL-10,and TGF-βin the four groups were observed.RESULTS In contrast to the successfully treated and normal control groups,both the incipient and relapse-refractory groups exhibited greater proportions of CD4-positive(+)Tregs(P<0.05),whereas the proportion of CD8+Tregs did not differ substantially between the groups.Serum levels of IL-35 and IL-10 in the incipient and relapsed-refractory groups were higher than those in the effectively treated and normal control groups(P<0.05).There was no statistically significant distinction in the expression level of TGF-βbetween the groups(P>0.05).The correlation between IL-35 and IL-10 concentrations was significantly positive,with a correlation coefficient of 0.531(P<0.05).The correlation between IL-35 and TGF-βconcentration was significantly positive,with a correlation coefficient of 0.375(P<0.05).The correlation between IL-10 and TGF-βconcentration was significantly positive,with a correlation coefficient of 0.185(P<0.05).The expression concentrations of IL-35,IL-10 and TGF-βwere apparently and positively correlated(P<0.05).CONCLUSION Tregs IL-35,and IL-10 may be closely associated with the occurrence and development of DLBCL and the detection of related indices may be helpful in the analysis of disease prognosis.
文摘Spurred by better understanding of disease biology,improvements in molecular diagnostics,and the development of targeted therapies,the treatment of acute myeloid leukemia(AML)has undergone significant evolution in recent years.Arguably,the most exciting shift has come from the success of treatment with the B-cell lymphoma-2 inhibitor venetoclax.When given in combination with a hypomethylating agent or low dose cytarabine,venetoclax demonstrates high response rates,some of which are durable.In spite of this,relapses after venetoclax treatment are common,and much interest exists in elucidating the mechanisms of resistance to the drug.Alterations in leukemic stem cell metabolism have been identified as a possible escape route,and clinical trials focusing on targeting metabolism in AML are ongoing.This review article highlights current research regarding venetoclax treatment and resistance in AML with a focus on cellular metabolism.
文摘目的探讨泛素特异性蛋白酶10(ubiquitin-specifi c protease 10,USP10)蛋白及其m RNA在人弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)与淋巴结反应性增生(reactive lymph node hyperplasia,RLH)中的表达及其临床意义。方法选取70例人DLBCL及70例RLH组织,采用组织芯片和免疫组织化学染色检测USP10的蛋白表达,分析其与DLBCL分子亚型及DLBCL预后相关分子之间的关系,并采用GEO数据库分析USP10 m RNA表达水平。结果 USP10在DLBCL组织中的阳性表达率显著高于RLH组织的B淋巴细胞,在非生发中心亚型(non-germinal center B-cell-like type,nonGCB)DLBCL组织中的阳性表达率明显高于生发中心亚型(germinal center B-cell-like type,GCB)DLBCL组织;Spearman等级相关分析显示,USP10与DLBCL预后相关分子中的CD5、Bcl-2蛋白表达呈正相关,而与p53,c-Myc及Ki67蛋白表达无相关性;USP10 m RNA在DLBCL组织及RLH组织中的表达差异。结论 USP10在DLBCL组织中的表达明显增加,尤其是在预后较差的non-GCB亚型或CD5阳性或无Bcl-2阳性的DLBCL中,表明该蛋白是DLBCL患者预后不良的分子标记物之一。
基金Supported by Specialized Research Fund for the Doctoral Program of Higher Education of China,No.20120142120048Natural Science Foundation of Hubei Province,China,No.2012FFB02308
文摘AIM: To investigate the association between endogenous hydrogen sulfide (H<sub>2</sub>S) and portal hypertension as well as its effect on vascular smooth muscle cells.
基金Supported by A grant from Universidad Nacional de Colombia
文摘AIM： To assess the significance of chromosome translo- cation t（11;18）（q21;q21）, B-cell lymphoma 10 （BCL-10） protein and He/icobacter py/ori （H. py/on） infection in gastric mucosa-associated lymphoid tissue （MALT） lymphoma in Colombia.
文摘BACKGROUND The concurrence of acute myeloid leukemia(AML)and chronic lymphocytic leukemia(CLL)is rare.Previous reports of such cases have focused mainly on clinical diagnosis and characteristics,so the mechanism remains unclear,and therapy options have been poorly explored.CASE SUMMARY Here,we report two cases of synchronous AML and CLL.Flow cytometry revealed two distinct abnormal cell populations(myeloblasts and lymphoid cells)according to scatter characteristics.CD5-positive B cell lymphoma with myeloid leukemia invasion was observed on lymph node biopsy.Chemotherapy regimens indicated for both AML and CLL were used in our patients,and our patients achieved complete response after chemotherapy.Next-generation sequencing of 88 genes was performed.CONCLUSION We conclude that early mutation and dysregulation at the hematopoietic stem cell stage and the accumulation of multiple rearrangements may cause the concurrence of CLL and AML.The treatment of infection and combination therapy aimed at the CLL component are significant in the management of patients with concurrent CLL and AML.
文摘【指示性摘要】IL-6由肿瘤细胞自分泌,与肿瘤侵袭性密切相关;TNF-α在机体肿瘤免疫应答中起到了重要作用,可引起肿瘤细胞坏死,且可诱导IL-6的表达;IFN-γ具有抗病毒,抗肿瘤和免疫调控的作用;IL-10具有双向免疫调节作用,在肿瘤环境中对免疫应答具有负向调节作用,同时也能发挥刺激作用。上述细胞因子在弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)的发生发展中或起到协同作用,并可能作为DLBCL诊断及预后的指标。
