Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods The pretreated human milk samples were adequately separated and quan...Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column （2.1×100 mm, 1.8 μm）. The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 mL/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESl＋ionization and detected in multiple reaction monitoring （MRM） with three acquisition functions. Results Calibration curves ranged from 0.5-160 ng/mL （thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal）, and 2.5-800 ng/mL （pantothenic acid, FAD and nicotinamide） （R^2=0.990-0.999）. The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification （LOQ） for all vitamins was between 0.25 and 3 lag/L. Conclusion This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk.展开更多
目的探讨B族维生素(维生素B1、B2、B6,烟酸)的摄入量与慢性便秘、慢性腹泻的关系。方法合并美国国家健康和营养调查数据库(National Health and Nutrition Examination Survey,NHANES)3个周期(2005-2006年、2007-2008年和2009-2010年)...目的探讨B族维生素(维生素B1、B2、B6,烟酸)的摄入量与慢性便秘、慢性腹泻的关系。方法合并美国国家健康和营养调查数据库(National Health and Nutrition Examination Survey,NHANES)3个周期(2005-2006年、2007-2008年和2009-2010年)的数据信息进行分析,共纳入13788名参与者,通过24 h饮食回忆访谈评估维生素B1、B2、B6,烟酸的摄入量。采用多元Logistic回归分析探讨B族维生素与慢性便秘、慢性腹泻的相关性,并进行了分层分析和曲线拟合相关分析。结果纳入的受试者中有2520人(18.28%)存在慢性便秘,2138人(15.51%)存在慢性腹泻。多元Logistic回归和分层分析结果显示,维生素B1、烟酸、维生素B6的摄入量与慢性便秘、慢性腹泻呈线性相关(P<0.05),每日摄入量的增加可降低慢性便秘的发生风险,但会增加慢性腹泻的发生风险(P<0.05)。结论维生素B1、B2、B6,烟酸与慢性便秘、慢性腹泻存在相关性。展开更多
目的检测新生儿B族链球菌(group B streptococcus,GBS)感染所致化脓性脑膜炎(purulent meningitis,PM)血清中维生素D、白细胞介素(interleukin,IL)-6、IL-10、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和C反应蛋白(c-reactive ...目的检测新生儿B族链球菌(group B streptococcus,GBS)感染所致化脓性脑膜炎(purulent meningitis,PM)血清中维生素D、白细胞介素(interleukin,IL)-6、IL-10、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和C反应蛋白(c-reactive protein,CRP)的表达水平,并探讨其临床价值。方法选取2017年5月至2020年5月在秦皇岛市第一医院出生的59例GBS感染的PM新生儿纳入观察组,同期59例非GBS感染的PM新生儿(晚发败血症)纳入对照组。检测所有受试者血清维生素D、CRP、IL-6、IL-10和TNF-α水平,并进行Pearson相关性分析;利用受试者操作特征(receiver operating characteristic,ROC)曲线分析血清维生素D和炎性细胞因子对新生儿GBS感染所致PM的诊断价值。统计学方法采用t检验、χ^(2)检验和Pearson相关性分析。结果观察组与对照组孕产妇胎膜早破[47.5%(28/59)与5.1%(3/59),χ^(2)=27.345]、产时窒息[52.5%(31/59)与18.6%(11/59),χ^(2)=14.787]和产褥感染[(44.1%(26/59)与(22.0%(13/59)),χ^(2)=6.473]的发生率比较,观察组明显高于对照组(P<0.05)。观察组血清维生素D水平显著低于对照组[(13.3±2.1)μg/L与(21.1±5.0)μg/L,t=11.345],IL-6[(87.1±14.5)μg/L与(63.9±11.9)μg/L,t=9.507]、IL-10[(49.6±15.2)μg/L与(29.3±10.0)μg/L,t=8.596]、TNF-α[(76.8±19.0)μg/L与(50.0±10.8)μg/L,t=9.410]和CRP[(21.5±5.0)μg/L与(13.7±3.7)μg/L,t=9.702]水平显著高于对照组(P值均<0.05)。Pearson相关性分析结果显示,观察组血清维生素D水平分别与IL-6、IL-10、TNF-α和CRP水平呈负相关(r=-0.662、-0.644、-0.564、-0.643,P<0.05);血清维生素D、IL-6、IL-10、TNF-α和CRP单独诊断GBS感染新生儿PM的曲线下面积(area under the curve,AUC)分别为0.831(95%CI:0.757~0.904)、0.887(95%CI:0.830~0.944)、0.859(95%CI:0.793~0.925)、0.888(95%CI:0.821~0.955)、0.879(95%CI:0.820~0.938),5项联合检测的AUC为0.991(95%CI:0.978~1.000)。结论GBS感染所致的PM新生儿血清中维生素D水平降低,炎性细胞因子水平增加,对于GBS感染所致的PM具有一定的辅助诊断价值。展开更多
基金supported by the National High Technology Research and Development Program of China(863 Program)(No.2010AA023004)
文摘Objective To determine ten B-vitamins in human milk by ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Methods The pretreated human milk samples were adequately separated and quantified within 11 min by UPLC-MS/MS with an Acquity UPLC HSS T3 column （2.1×100 mm, 1.8 μm）. The mobile phase was a gradient of 2.5 mmol/L ammonium formate aqueous solution and acetonitrile at a flow rate of 0.35 mL/min. Stable isotope internal standards were used in the analysis, to correct for the method variability, including matrix and ionization effects. The homogenized human milk samples were deproteinzed using methanol, unknown contaminants were extracted with diethyl ether and hydrophobic phase was discarded. The analytes were monitored via ESl＋ionization and detected in multiple reaction monitoring （MRM） with three acquisition functions. Results Calibration curves ranged from 0.5-160 ng/mL （thiamin, riboflavin, biotin, nicotinic acid, pyridoxine, pyridoxamine, pyridoxal）, and 2.5-800 ng/mL （pantothenic acid, FAD and nicotinamide） （R^2=0.990-0.999）. The relative recovery ranged from 80.1% to 120.2%; accuracy was determined to be 98.3% to 108.0%. Intra-day and inter-day variation were 3.4%-19.9% and 5.9%-18.1%, respectively. The limit of quantification （LOQ） for all vitamins was between 0.25 and 3 lag/L. Conclusion This method was successfully applied for simultaneous analysis of ten B-vitamins in human milk.
文摘目的探讨B族维生素(维生素B1、B2、B6,烟酸)的摄入量与慢性便秘、慢性腹泻的关系。方法合并美国国家健康和营养调查数据库(National Health and Nutrition Examination Survey,NHANES)3个周期(2005-2006年、2007-2008年和2009-2010年)的数据信息进行分析,共纳入13788名参与者,通过24 h饮食回忆访谈评估维生素B1、B2、B6,烟酸的摄入量。采用多元Logistic回归分析探讨B族维生素与慢性便秘、慢性腹泻的相关性,并进行了分层分析和曲线拟合相关分析。结果纳入的受试者中有2520人(18.28%)存在慢性便秘,2138人(15.51%)存在慢性腹泻。多元Logistic回归和分层分析结果显示,维生素B1、烟酸、维生素B6的摄入量与慢性便秘、慢性腹泻呈线性相关(P<0.05),每日摄入量的增加可降低慢性便秘的发生风险,但会增加慢性腹泻的发生风险(P<0.05)。结论维生素B1、B2、B6,烟酸与慢性便秘、慢性腹泻存在相关性。
文摘目的检测新生儿B族链球菌(group B streptococcus,GBS)感染所致化脓性脑膜炎(purulent meningitis,PM)血清中维生素D、白细胞介素(interleukin,IL)-6、IL-10、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和C反应蛋白(c-reactive protein,CRP)的表达水平,并探讨其临床价值。方法选取2017年5月至2020年5月在秦皇岛市第一医院出生的59例GBS感染的PM新生儿纳入观察组,同期59例非GBS感染的PM新生儿(晚发败血症)纳入对照组。检测所有受试者血清维生素D、CRP、IL-6、IL-10和TNF-α水平,并进行Pearson相关性分析;利用受试者操作特征(receiver operating characteristic,ROC)曲线分析血清维生素D和炎性细胞因子对新生儿GBS感染所致PM的诊断价值。统计学方法采用t检验、χ^(2)检验和Pearson相关性分析。结果观察组与对照组孕产妇胎膜早破[47.5%(28/59)与5.1%(3/59),χ^(2)=27.345]、产时窒息[52.5%(31/59)与18.6%(11/59),χ^(2)=14.787]和产褥感染[(44.1%(26/59)与(22.0%(13/59)),χ^(2)=6.473]的发生率比较,观察组明显高于对照组(P<0.05)。观察组血清维生素D水平显著低于对照组[(13.3±2.1)μg/L与(21.1±5.0)μg/L,t=11.345],IL-6[(87.1±14.5)μg/L与(63.9±11.9)μg/L,t=9.507]、IL-10[(49.6±15.2)μg/L与(29.3±10.0)μg/L,t=8.596]、TNF-α[(76.8±19.0)μg/L与(50.0±10.8)μg/L,t=9.410]和CRP[(21.5±5.0)μg/L与(13.7±3.7)μg/L,t=9.702]水平显著高于对照组(P值均<0.05)。Pearson相关性分析结果显示,观察组血清维生素D水平分别与IL-6、IL-10、TNF-α和CRP水平呈负相关(r=-0.662、-0.644、-0.564、-0.643,P<0.05);血清维生素D、IL-6、IL-10、TNF-α和CRP单独诊断GBS感染新生儿PM的曲线下面积(area under the curve,AUC)分别为0.831(95%CI:0.757~0.904)、0.887(95%CI:0.830~0.944)、0.859(95%CI:0.793~0.925)、0.888(95%CI:0.821~0.955)、0.879(95%CI:0.820~0.938),5项联合检测的AUC为0.991(95%CI:0.978~1.000)。结论GBS感染所致的PM新生儿血清中维生素D水平降低,炎性细胞因子水平增加,对于GBS感染所致的PM具有一定的辅助诊断价值。
