[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers se...[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.展开更多
基金Supported by Hunan Provincial Natural Science Foundation of China(2008JJ3035)~~
文摘[Objective] This study aimed to explore the genetic relationship of purple tsai-tai germplasms using SSR molecular markers. [Method] SSR analysis of 45 purple tsai-tai samples was conducted with 65 pairs of primers selected from cabbage primers, and the cluster analysis was carried out. [Result] A total of 23 pairs of SSR primers were screened; cluster analysis showed that the genetic relationship of purple tsai-tai germplasms had relatively significantly locality, and the 45 purple tsaitai samples can be divided into three groups of Sichuan, Hunan and Hubei; furthermore, the similarity coefficients of 45 purple tsai-tai samples were all greater than 0.5 （ranging from 0.547 0 to 0.910 7）, indicating that the closer the genetic relationship among purple tsai-tai samples is, the narrower the genetic basis will be. [Conclusion] This study provides a theoretical and technical basis for the identification, protection and breeding of the resources of local varieties of seaweed sprouts. This study provided theoretical and technical basis for the identification, protection, breeding and utilization of local purple tsai-tai resource.
