Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:...Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:A rat model of lower extremity ASO was established,and blood samples from patients with lower extremity ASO before and after surgery were obtained.ELISA was used to detect interleukin 6(IL-6),IL-10,and IL-17.Real-time RCR and Western blot analyses were used to detect Foxp3,IL-6,IL-10,and IL-17 expression.Moreover,flow cytometry was applied to detect the Th17/Treg ratio and M1/M2 ratio.Results:Compared with the control group,the iliac artery wall of ASO rats showed significant hyperplasia,and the concentrations of cholesterol and triglyceride were significantly increased(P<0.01),indicating the successful establishment of ASO.Moreover,the levels of IL-6 and IL-17 in ASO rats were pronouncedly increased(P<0.05),while the IL-10 level was significantly decreased(P<0.05).In addition to increased IL-6 and IL-17 levels,the mRNA and protein levels of Foxp3 and IL-10 in ASO rats were significantly decreased compared with the control group.The Th17/Treg and M1/M2 ratios in the ASO group were markedly increased(P<0.05).These alternations were also observed in ASO patients.After endovascular surgery(such as percutaneous transluminal angioplasty and arterial stenting),all these changes were significantly improved(P<0.05).Conclusions:The Th17/Treg and M1/M2 ratios were significantly increased in ASO,and surgery can effectively improve the balance of Th17/Treg,and reduce the ratio of M1/M2,and the expression of inflammatory factors.展开更多
The Fermat–Pramanik series are like below: .The mathematical principle has been established by factorization principle. The Fermat-Pramanik tree can be grown. It produces branched Fermat-Pramanik series using same pr...The Fermat–Pramanik series are like below: .The mathematical principle has been established by factorization principle. The Fermat-Pramanik tree can be grown. It produces branched Fermat-Pramanik series using same principle making Fermat-Pramanik chain. Branched chain can be propagated at any point of the main chain with indefinite length using factorization principle as follows: Same principle is applicable for integer solutions of A<sup>M</sup>+B<sup>2</sup>=C<sup>2</sup>which produces series of the type . It has been shown that this equation is solvable with N{A, B, C, M}. where , , M=M<sub>1</sub>+M<sub>2</sub> and M<sub>1</sub>>M<sub>2</sub>. Subsequently, it has been shown that using M= M<sub>1</sub>+M<sub>2</sub>+M<sub>3</sub>+... The combinations of Ms should be taken so that the values of both the parts (C<sub>n</sub>+B<sub>n</sub>) and (C<sub>n</sub>-B<sub>n</sub>) should be even or odd for obtaining Z{B,C}. Hence, it has been shown that the Fermat triple can generate a) Fermat-Pramanik multiplate, b) Fermat-Pramanik Branched multiplate and c) Fermat-Pramanik deductive series. All these formalisms are useful for development of new principle of cryptography. .展开更多
基金supported by Natural Science Foundation of Hainan Province(820MS135)Hainan Provincial Health Commission 2023 Provincial Key Clinical Discipline(Clinical Medical Center)Construction Unit Fund Project(Qiongwei Yihan[2022]No.341)Hainan Provincial Health Technology Innovation Joint Project(WSJK2024MS209).
文摘Objective:To explore the balance of peripheral blood T helper 17 cells/regulatory T cell(Th17/Treg)ratio and the polarization ratio of M1 and M2 macrophages in lower extremity arteriosclerosis obliterans(ASO).Methods:A rat model of lower extremity ASO was established,and blood samples from patients with lower extremity ASO before and after surgery were obtained.ELISA was used to detect interleukin 6(IL-6),IL-10,and IL-17.Real-time RCR and Western blot analyses were used to detect Foxp3,IL-6,IL-10,and IL-17 expression.Moreover,flow cytometry was applied to detect the Th17/Treg ratio and M1/M2 ratio.Results:Compared with the control group,the iliac artery wall of ASO rats showed significant hyperplasia,and the concentrations of cholesterol and triglyceride were significantly increased(P<0.01),indicating the successful establishment of ASO.Moreover,the levels of IL-6 and IL-17 in ASO rats were pronouncedly increased(P<0.05),while the IL-10 level was significantly decreased(P<0.05).In addition to increased IL-6 and IL-17 levels,the mRNA and protein levels of Foxp3 and IL-10 in ASO rats were significantly decreased compared with the control group.The Th17/Treg and M1/M2 ratios in the ASO group were markedly increased(P<0.05).These alternations were also observed in ASO patients.After endovascular surgery(such as percutaneous transluminal angioplasty and arterial stenting),all these changes were significantly improved(P<0.05).Conclusions:The Th17/Treg and M1/M2 ratios were significantly increased in ASO,and surgery can effectively improve the balance of Th17/Treg,and reduce the ratio of M1/M2,and the expression of inflammatory factors.
文摘The Fermat–Pramanik series are like below: .The mathematical principle has been established by factorization principle. The Fermat-Pramanik tree can be grown. It produces branched Fermat-Pramanik series using same principle making Fermat-Pramanik chain. Branched chain can be propagated at any point of the main chain with indefinite length using factorization principle as follows: Same principle is applicable for integer solutions of A<sup>M</sup>+B<sup>2</sup>=C<sup>2</sup>which produces series of the type . It has been shown that this equation is solvable with N{A, B, C, M}. where , , M=M<sub>1</sub>+M<sub>2</sub> and M<sub>1</sub>>M<sub>2</sub>. Subsequently, it has been shown that using M= M<sub>1</sub>+M<sub>2</sub>+M<sub>3</sub>+... The combinations of Ms should be taken so that the values of both the parts (C<sub>n</sub>+B<sub>n</sub>) and (C<sub>n</sub>-B<sub>n</sub>) should be even or odd for obtaining Z{B,C}. Hence, it has been shown that the Fermat triple can generate a) Fermat-Pramanik multiplate, b) Fermat-Pramanik Branched multiplate and c) Fermat-Pramanik deductive series. All these formalisms are useful for development of new principle of cryptography. .
