Biophoton emission is produced by all living systems;this emission pattern has been shown to be altered by the presence of an electromagnetic field (EMF). Cultures of B16-BL6 cells were exposed to a weak EMF produced ...Biophoton emission is produced by all living systems;this emission pattern has been shown to be altered by the presence of an electromagnetic field (EMF). Cultures of B16-BL6 cells were exposed to a weak EMF produced by a specially constructed EM generator, called the “Resonator”, for one hour. This EM generator incorporates multiple geometric ratios in its design, including the golden ratio (phi), pi, root 2, root 3, and root 5. It has been used previously to purify water of toxins. There was a significant decrease in mean photon counts from B16-BL6 cells exposed at a distance of 1 m compared to those exposed at 0 m. Alterations in the spectral power density variability were also observed in the 8 - 10 Hz range. The EM generator may have an impact on the viability of the exposed cell cultures, but only at specific distances.展开更多
We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatm...We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatment of cells with various concentrations of CSE (0, 0.1, 0.3, and 1%) dose-dependently reduced the number of lung metastatic nodules 14 days after tumor injection. To elucidate the mechanism of this anti-metastatic effect of CSE, we examined the invasion and migration activities of B16-BL6 cells pretreated with CSE for three hours in vitro. CSE significantly reduced the invasion of cells at 1% and the migration at 0.3% and 1%. Under the same pretreatment conditions, CSE had no effect on the proliferation of cells. These findings suggest that CSE contains some ingredients that suppress hematogenic lung metastasis via inhibition of the invasion and migration activities of mouse melanoma cells.展开更多
文摘Biophoton emission is produced by all living systems;this emission pattern has been shown to be altered by the presence of an electromagnetic field (EMF). Cultures of B16-BL6 cells were exposed to a weak EMF produced by a specially constructed EM generator, called the “Resonator”, for one hour. This EM generator incorporates multiple geometric ratios in its design, including the golden ratio (phi), pi, root 2, root 3, and root 5. It has been used previously to purify water of toxins. There was a significant decrease in mean photon counts from B16-BL6 cells exposed at a distance of 1 m compared to those exposed at 0 m. Alterations in the spectral power density variability were also observed in the 8 - 10 Hz range. The EM generator may have an impact on the viability of the exposed cell cultures, but only at specific distances.
文摘We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatment of cells with various concentrations of CSE (0, 0.1, 0.3, and 1%) dose-dependently reduced the number of lung metastatic nodules 14 days after tumor injection. To elucidate the mechanism of this anti-metastatic effect of CSE, we examined the invasion and migration activities of B16-BL6 cells pretreated with CSE for three hours in vitro. CSE significantly reduced the invasion of cells at 1% and the migration at 0.3% and 1%. Under the same pretreatment conditions, CSE had no effect on the proliferation of cells. These findings suggest that CSE contains some ingredients that suppress hematogenic lung metastasis via inhibition of the invasion and migration activities of mouse melanoma cells.
