采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是...采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。展开更多
To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen produc...To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen production has a correlation with cell growth and the consumption of glucose and soluble protein. The optimum pH for cell growth is 4.5±0.15. At acidic pH 4.0±0.15, the bacteria has the maximum accumulated hydrogen volume of 2382 ml/L culture and the maximum hydrogen evolution rate of 339.9 ml/L culture·h with 1% glucose. The optimum temperature for cell growth and hydrogen production is 35℃. In addition, fermentative hydrogen-producing bacterial strain B49 can generate hydrogen from the decomposition of other organic substrates such as wheat, soybean, corn, and potato. Moreover, it can also produce hydrogen from molasses wastewater and brewage wastewater, and hydrogen yields are 137.9 ml H 2/g COD and 49.9 ml H 2/g COD, respectively.展开更多
To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method,...To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method, with the addition of a small amount of calcium alginate in a column reactor obtain hydrogen yield of 2.31 mol H2/mol glucose and hydrogen evolution rate of 1435.4 ml/L culture·h respectively at medium retention time of 2.0 h with a medium containing 10g glucose/L. Moreover, as the cell density in gel beads is increased to 8%, hydrogen yield and hydrogen evolution rate for 10g glucose/L are 2.34 mol H2/mol glucose and 2912.4 ml/L culture·h respectively at medium retention time of 1.0 h, and for molasses wastewater COD of 7505.9 mg/L hydrogen production potential of 205.6 ml/g COD and hydrogen evolution rate of 2057.7 ml/L culture·h at hydraulic retention time of 0.75 h are observed. In the continuous culture pH value keeps around 3.9 by self regulating.展开更多
Batch experiments were conducted to investigate the effects of magnesium on glucose metabolism, including growth and hydrogen-producing capacity of fermentative hydrogen-producing bacterial strain B49. These abilities...Batch experiments were conducted to investigate the effects of magnesium on glucose metabolism, including growth and hydrogen-producing capacity of fermentative hydrogen-producing bacterial strain B49. These abilities were enhanced with an increase in magnesium concentration. At the end of fermentation from （10 g/L） glucose, for 10 mg/L MgCl2·6H2O the cell growth in terms of optical density （OD） at 600nm was 0.46, the ratio of ethanol amount （mg/L） to acetate amount （mg/L） was 1.1, and the accumulated hydrogen volume was 934.9 mL H2/L culture; for 200 mg/L of MgCl2·6H2O OD600 nm was increased to 1.34. The accumulated hydrogen volume was increased to 2 360.5 mL H2/L culture, the ratio of ethanol amount （mg/L） to acetate amount （mg/L） was increased to 1.3 and polysaccharide was decreased to 2.5 mg/L. Moreover, the magnesium solution addition to the medium at different fermentation times affected hydrogen-producing ability. However, the later the addition time was postponed, the less the effect was on hydrogen evolution. Further experiments confirmed the enhancement was dependent on magnesium ions and not on the other inorganic ions such as SO42- or Cl-, which constituted the magnesium salts.展开更多
目的观测黏附分子CD49b和负性调节分子淋巴细胞活化基因3(LAG-3)在反复自发性流产(RSA)患者CD14+细胞上的表达。方法收集7例正常对照者和12例RSA患者外周血5 m L,分离外周血单个核细胞(PBMC)和血浆,流式细胞术检测PBMC中CD14+细胞表面CD...目的观测黏附分子CD49b和负性调节分子淋巴细胞活化基因3(LAG-3)在反复自发性流产(RSA)患者CD14+细胞上的表达。方法收集7例正常对照者和12例RSA患者外周血5 m L,分离外周血单个核细胞(PBMC)和血浆,流式细胞术检测PBMC中CD14+细胞表面CD49b和LAG-3的表达;ELISA检测血浆中细胞因子白细胞介素10(IL-10)和转化生长因子β(TGF-β)的水平。结果 RSA患者外周血中,单核细胞的比例与正常对照组相比无显著性差异;CD14+CD49b+、CD14+LAG-3+、CD14+CD49b+LAG-3+细胞的百分比均低于正常对照组。血浆中,TGF-β的水平低于正常对照组;IL-10的水平无显著差异。结论 RSA患者外周血CD14+细胞表面CD49b和LAG-3的表达和血浆中TGF-β的水平均显著降低。展开更多
Integrins are cell adhesion molecules that are composed of an alpha(a)subunit and a beta(b)subunit with affinity for different extracellular membrane components.The integrin family includes 24 known members that activ...Integrins are cell adhesion molecules that are composed of an alpha(a)subunit and a beta(b)subunit with affinity for different extracellular membrane components.The integrin family includes 24 known members that actively regulate cellular growth,differentiation,and apoptosis.Each integrin heterodimer has a particular function in defined contexts as well as some partially overlapping features with other members in the family.As many reviews have covered the general integrin family in molecular and cellular studies in life science,this review will focus on the specific regulation,function,and signaling of integrin a2 subunit(CD49b,VLA-2;encoded by the gene ITGA2)in partnership with b1(CD29)subunit in normal and cancer cells.Its roles in cell adhesion,cell motility,angiogenesis,stemness,and immune/blood cell regulations are discussed.The pivotal role of integrin a2 in many diseases such as cancer suggests its potential to be used as a novel therapeutic target.展开更多
Cancer metastasis is largely incurable and accounts for 90%of breast cancer deaths,especially for the aggressive basal-like or triple negative breast cancer(TNBC).Combining patient database analyses and functional stu...Cancer metastasis is largely incurable and accounts for 90%of breast cancer deaths,especially for the aggressive basal-like or triple negative breast cancer(TNBC).Combining patient database analyses and functional studies,we examined the association of integrin family members with clinical outcomes as well as their connection with previously identified microRNA regulators of metastasis,such as miR-206 that inhibits stemness and metastasis of TNBC.Here we report that the integrin receptor CD49b-encoding ITGA2,a direct target of miR-206,promotes breast cancer stemness and metastasis.ITGA2 knockdown sup-pressed self-renewal related mammosphere formation and pluripotency marker expression,in-hibited cell cycling,compromised migration and invasion,and therefore decreased lung metastasis of breast cancer.ITGA2 overexpression reversed miR-206-caused cell cycle arrest in G1.RNA sequencing analyses revealed that ITGA2 knockdown inhibits genes related to cell cycle regulation and lipid metabolism,including CCND1 and ACLY as representative targets,respectively.Knockdown of CCND1 or ACLY inhibits mammosphere formation of breast cancer cells.Overexpression of CCND1 rescues the phenotype of ITGA2 knockdown-induced cell cycle arrest.ACLY-encoded ATP citrate lyase is essential to maintain cellular acetyl-CoA levels.CCND1 knockdown further mimics 1TGA2 knodkdown in abolishing lung colonization of breast cancer cells.We identified that the low levels of miR-206 as well as high expression levels of 1TGA2,ACLY and CCND1 are associated with an unf avor able relapse-free survival of the pa-tients with estrogen receptor-negative or high grade breast cancer,especially basal-like or TNBC,possibly serving as potential biomarkers of cancer stemness and thera peutic targets of breast cancer metastasis.展开更多
文摘采用间歇发酵实验,研究了葡萄糖浓度、接种量、温度、氮源、不同有机底物对发酵产氢产酸细菌新菌种B49(AF48ll48 in EMBL)生物产氢的影响。结果表明,接种量影响B49的产氢;B49生长和产氢适宜温度均为35℃;B49不能利用无机氮源,而有机氮是B49生长、产氢的适宜氮源;葡萄糖是B49发酵产氢的最适宜底物,当浓度为10g/L时,B49的葡萄糖利用率为100%,氢气得率为1.69mol H_2/mol glucose;此外,B49可利用小麦、大豆、玉米、土豆及糖蜜废水和啤酒废水产氢,其中利用糖蜜废水、啤酒废水产氢分别为137.9ml H_2/ g COD和49.9ml H_2/g COD。
文摘To investigate the characteristics of hydrogen production by a novel fermentative hydrogen-producing bacterial strain B49 (AF481148 in EMBL), batch experiments are conducted under different conditions. Hydrogen production has a correlation with cell growth and the consumption of glucose and soluble protein. The optimum pH for cell growth is 4.5±0.15. At acidic pH 4.0±0.15, the bacteria has the maximum accumulated hydrogen volume of 2382 ml/L culture and the maximum hydrogen evolution rate of 339.9 ml/L culture·h with 1% glucose. The optimum temperature for cell growth and hydrogen production is 35℃. In addition, fermentative hydrogen-producing bacterial strain B49 can generate hydrogen from the decomposition of other organic substrates such as wheat, soybean, corn, and potato. Moreover, it can also produce hydrogen from molasses wastewater and brewage wastewater, and hydrogen yields are 137.9 ml H 2/g COD and 49.9 ml H 2/g COD, respectively.
文摘To improve the hydrogen evolution rate in continuous hydrogen production of a novel fermentative hydrogen producing bacteria strain B49 (AF481148 in EMBL), 4 % immobilized cells by polyvinyl alcohol boric acid method, with the addition of a small amount of calcium alginate in a column reactor obtain hydrogen yield of 2.31 mol H2/mol glucose and hydrogen evolution rate of 1435.4 ml/L culture·h respectively at medium retention time of 2.0 h with a medium containing 10g glucose/L. Moreover, as the cell density in gel beads is increased to 8%, hydrogen yield and hydrogen evolution rate for 10g glucose/L are 2.34 mol H2/mol glucose and 2912.4 ml/L culture·h respectively at medium retention time of 1.0 h, and for molasses wastewater COD of 7505.9 mg/L hydrogen production potential of 205.6 ml/g COD and hydrogen evolution rate of 2057.7 ml/L culture·h at hydraulic retention time of 0.75 h are observed. In the continuous culture pH value keeps around 3.9 by self regulating.
文摘Batch experiments were conducted to investigate the effects of magnesium on glucose metabolism, including growth and hydrogen-producing capacity of fermentative hydrogen-producing bacterial strain B49. These abilities were enhanced with an increase in magnesium concentration. At the end of fermentation from （10 g/L） glucose, for 10 mg/L MgCl2·6H2O the cell growth in terms of optical density （OD） at 600nm was 0.46, the ratio of ethanol amount （mg/L） to acetate amount （mg/L） was 1.1, and the accumulated hydrogen volume was 934.9 mL H2/L culture; for 200 mg/L of MgCl2·6H2O OD600 nm was increased to 1.34. The accumulated hydrogen volume was increased to 2 360.5 mL H2/L culture, the ratio of ethanol amount （mg/L） to acetate amount （mg/L） was increased to 1.3 and polysaccharide was decreased to 2.5 mg/L. Moreover, the magnesium solution addition to the medium at different fermentation times affected hydrogen-producing ability. However, the later the addition time was postponed, the less the effect was on hydrogen evolution. Further experiments confirmed the enhancement was dependent on magnesium ions and not on the other inorganic ions such as SO42- or Cl-, which constituted the magnesium salts.
文摘目的观测黏附分子CD49b和负性调节分子淋巴细胞活化基因3(LAG-3)在反复自发性流产(RSA)患者CD14+细胞上的表达。方法收集7例正常对照者和12例RSA患者外周血5 m L,分离外周血单个核细胞(PBMC)和血浆,流式细胞术检测PBMC中CD14+细胞表面CD49b和LAG-3的表达;ELISA检测血浆中细胞因子白细胞介素10(IL-10)和转化生长因子β(TGF-β)的水平。结果 RSA患者外周血中,单核细胞的比例与正常对照组相比无显著性差异;CD14+CD49b+、CD14+LAG-3+、CD14+CD49b+LAG-3+细胞的百分比均低于正常对照组。血浆中,TGF-β的水平低于正常对照组;IL-10的水平无显著差异。结论 RSA患者外周血CD14+细胞表面CD49b和LAG-3的表达和血浆中TGF-β的水平均显著降低。
基金This manuscript is partially supported by the funds from the National Institutes of Health/National Cancer Institute(NIH/NCI)R00CA160638(H.L.)its Supplement for Diversity(V.A.),American Cancer Society 127951-RSG-15-025-01-CSM(H.L.)+1 种基金Susan G.Komen Foundation CCR15332826(H.L.)Department of Defense W81XWH-16-1-0021(H.L.).
文摘Integrins are cell adhesion molecules that are composed of an alpha(a)subunit and a beta(b)subunit with affinity for different extracellular membrane components.The integrin family includes 24 known members that actively regulate cellular growth,differentiation,and apoptosis.Each integrin heterodimer has a particular function in defined contexts as well as some partially overlapping features with other members in the family.As many reviews have covered the general integrin family in molecular and cellular studies in life science,this review will focus on the specific regulation,function,and signaling of integrin a2 subunit(CD49b,VLA-2;encoded by the gene ITGA2)in partnership with b1(CD29)subunit in normal and cancer cells.Its roles in cell adhesion,cell motility,angiogenesis,stemness,and immune/blood cell regulations are discussed.The pivotal role of integrin a2 in many diseases such as cancer suggests its potential to be used as a novel therapeutic target.
基金This manuscript has been partially supported by NIH/NCI grants R00CA160638 and R01CA245699(H.L.),and Supple-ment for Diversity(V.A.),T32 CA080621-15(R.T.),and R01CA213843(R.A.K),American Cancer Society grant ACS127951-RSG-15-025-01-CSM(H.L.)the Susan G.Komen Foundation CCR15332826(H.L.)and CCR18548501(X.L.)+3 种基金the Department of Defense W81XWH-16-1-0021(H.L.)the Lynn Sage Cancer Research Foundation(X.L.and H.L.)North-western University’s Endocrinology Training Grant T32DK007169-39(A.H.)and start-up funds fromCaseWestern Reserve University and at Northwestern University(H.L.).
文摘Cancer metastasis is largely incurable and accounts for 90%of breast cancer deaths,especially for the aggressive basal-like or triple negative breast cancer(TNBC).Combining patient database analyses and functional studies,we examined the association of integrin family members with clinical outcomes as well as their connection with previously identified microRNA regulators of metastasis,such as miR-206 that inhibits stemness and metastasis of TNBC.Here we report that the integrin receptor CD49b-encoding ITGA2,a direct target of miR-206,promotes breast cancer stemness and metastasis.ITGA2 knockdown sup-pressed self-renewal related mammosphere formation and pluripotency marker expression,in-hibited cell cycling,compromised migration and invasion,and therefore decreased lung metastasis of breast cancer.ITGA2 overexpression reversed miR-206-caused cell cycle arrest in G1.RNA sequencing analyses revealed that ITGA2 knockdown inhibits genes related to cell cycle regulation and lipid metabolism,including CCND1 and ACLY as representative targets,respectively.Knockdown of CCND1 or ACLY inhibits mammosphere formation of breast cancer cells.Overexpression of CCND1 rescues the phenotype of ITGA2 knockdown-induced cell cycle arrest.ACLY-encoded ATP citrate lyase is essential to maintain cellular acetyl-CoA levels.CCND1 knockdown further mimics 1TGA2 knodkdown in abolishing lung colonization of breast cancer cells.We identified that the low levels of miR-206 as well as high expression levels of 1TGA2,ACLY and CCND1 are associated with an unf avor able relapse-free survival of the pa-tients with estrogen receptor-negative or high grade breast cancer,especially basal-like or TNBC,possibly serving as potential biomarkers of cancer stemness and thera peutic targets of breast cancer metastasis.
