BACKGROUND Ankylosing spondylitis(AS)is strongly associated with the human leukocyte antigen(HLA)B27 haplotype.In regions where conventional polymerase chain reaction for HLA typing is available for antigens such as H...BACKGROUND Ankylosing spondylitis(AS)is strongly associated with the human leukocyte antigen(HLA)B27 haplotype.In regions where conventional polymerase chain reaction for HLA typing is available for antigens such as HLA B27 or HLA B51,it is common to perform the HLA B27 test for evaluation of AS.While HLA B27-associated clustered occurrences of AS have been reported in families,we report the first case series of HLA B51-related occurrences of AS in a family.CASE SUMMARY A father and his daughters were diagnosed with AS and did not have the HLA B27 haplotype.Although they were positive for HLA B51,they exhibited no signs of Behçet’s disease(BD).Of the five daughters,one had AS,and three,including the daughter with AS,were positive for HLA B51.The two daughters with the HLA B51 haplotype(excluding the daughter with AS)exhibited bilateral grade 1 sacroiliitis,whereas the daughters without the HLA B51 haplotype did not have sacroiliitis.Thus,this Korean family exhibited a strong association with the HLA B51 haplotype and clinical sacroiliitis,irrespective of the symptoms of BD.CONCLUSION It is advisable to check for HLA B51 positivity in patients with AS/spondyloarthropathy who test negative for HLA B27.展开更多
近年来,三唑类杀菌剂成为生产上防治小麦赤霉病(Fusarium Head Blight,FHB)的主要药剂,主要作用于甾醇生物合成中的14α-脱甲基酶(CYP51),使真菌麦角甾醇的合成受阻,从而破坏细胞膜功能,起到杀菌的功能。本研究的目的是采用结构生物学...近年来,三唑类杀菌剂成为生产上防治小麦赤霉病(Fusarium Head Blight,FHB)的主要药剂,主要作用于甾醇生物合成中的14α-脱甲基酶(CYP51),使真菌麦角甾醇的合成受阻,从而破坏细胞膜功能,起到杀菌的功能。本研究的目的是采用结构生物学的方法探究禾谷镰刀菌(Fusarium graminearum)中14α-脱甲基酶(FgCYP51B)的三维结构、生化功能以及其与三唑类杀菌剂的互作机制,进一步阐释禾谷镰刀菌对三唑类杀菌剂潜在的抗性风险。以禾谷镰刀菌(F.graminearum)的cDNA为模板,根据14α-脱甲基酶基因(FgCYP51B)序列设计特异性引物进行PCR扩增,克隆了FgCYP51B基因并构建了3个全长表达载体pHAT2-CYP51B、pETM-20-CYP51B和pETM-30-CYP51B以及3个蛋白截短体表达载体pHAT2-CYP51B^(50-527),pETM-20-CYP51B^(50-527)和pETM-30-CYP51B^(50-527),将重组表达载体转化到大肠杆菌BL21(DE3)和Rosetta(DE3),并利用异丙基硫代半乳糖苷(IPTG)进行诱导表达。结果表明:全长FgCYP51B蛋白在BL21(DE3)和Rosetta(DE3)中均不表达;重组蛋白pHAT2-CYP51B^(50-527)在大肠杆菌中不表达,而重组蛋白pETM-20-CYP51B^(50-527)和pETM-30-CYP51B^(50-527)成功表达,并通过亲和层析、离子交换与分子筛对重组蛋白进行纯化。该蛋白的表达与纯化为其结构功能的研究奠定了基础。展开更多
文摘BACKGROUND Ankylosing spondylitis(AS)is strongly associated with the human leukocyte antigen(HLA)B27 haplotype.In regions where conventional polymerase chain reaction for HLA typing is available for antigens such as HLA B27 or HLA B51,it is common to perform the HLA B27 test for evaluation of AS.While HLA B27-associated clustered occurrences of AS have been reported in families,we report the first case series of HLA B51-related occurrences of AS in a family.CASE SUMMARY A father and his daughters were diagnosed with AS and did not have the HLA B27 haplotype.Although they were positive for HLA B51,they exhibited no signs of Behçet’s disease(BD).Of the five daughters,one had AS,and three,including the daughter with AS,were positive for HLA B51.The two daughters with the HLA B51 haplotype(excluding the daughter with AS)exhibited bilateral grade 1 sacroiliitis,whereas the daughters without the HLA B51 haplotype did not have sacroiliitis.Thus,this Korean family exhibited a strong association with the HLA B51 haplotype and clinical sacroiliitis,irrespective of the symptoms of BD.CONCLUSION It is advisable to check for HLA B51 positivity in patients with AS/spondyloarthropathy who test negative for HLA B27.
文摘近年来,三唑类杀菌剂成为生产上防治小麦赤霉病(Fusarium Head Blight,FHB)的主要药剂,主要作用于甾醇生物合成中的14α-脱甲基酶(CYP51),使真菌麦角甾醇的合成受阻,从而破坏细胞膜功能,起到杀菌的功能。本研究的目的是采用结构生物学的方法探究禾谷镰刀菌(Fusarium graminearum)中14α-脱甲基酶(FgCYP51B)的三维结构、生化功能以及其与三唑类杀菌剂的互作机制,进一步阐释禾谷镰刀菌对三唑类杀菌剂潜在的抗性风险。以禾谷镰刀菌(F.graminearum)的cDNA为模板,根据14α-脱甲基酶基因(FgCYP51B)序列设计特异性引物进行PCR扩增,克隆了FgCYP51B基因并构建了3个全长表达载体pHAT2-CYP51B、pETM-20-CYP51B和pETM-30-CYP51B以及3个蛋白截短体表达载体pHAT2-CYP51B^(50-527),pETM-20-CYP51B^(50-527)和pETM-30-CYP51B^(50-527),将重组表达载体转化到大肠杆菌BL21(DE3)和Rosetta(DE3),并利用异丙基硫代半乳糖苷(IPTG)进行诱导表达。结果表明:全长FgCYP51B蛋白在BL21(DE3)和Rosetta(DE3)中均不表达;重组蛋白pHAT2-CYP51B^(50-527)在大肠杆菌中不表达,而重组蛋白pETM-20-CYP51B^(50-527)和pETM-30-CYP51B^(50-527)成功表达,并通过亲和层析、离子交换与分子筛对重组蛋白进行纯化。该蛋白的表达与纯化为其结构功能的研究奠定了基础。
