Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gast...Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.展开更多
目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的...目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的表达水平。通过直接ROC分析评估FAS和B7-H4分别对sPE的诊断价值和不良心血管结局的预测价值。通过二元logistics回归分析因子预测概率值,然后进行ROC分析,评估AS和B7-H4联合对sPE的诊断价值和不良心血管结局的预测价值。使用sPE血清培养心肌细胞,检测细胞中的肥大因子和纤维化因子。结果:GSE2347219数据集分析结果显示,FAS和B7-H4在sPE胎盘组织中上调。血清检测结果表明,FAS和B7-H4在sPE血清中上调。直接ROC分析结果表明,FAS和B7-H4可以区分sPE病例与正常孕妇,而且可以预测不良心血管结局。二元logistics回归分析和ROC分析结果表明,B7-H4联合FAS可以有效地区分sPE病例与正常孕妇并有效预测不良心血管结局。sPE血清培养可以升高心肌细胞中的肥大因子(ANP、BNP和β-MHC)和纤维化因子COL1A1的水平。结论:B7-H4 (VTCN1)联合FAS可以作为sPE患者的诊断因子和不良心血管结局的预测因子。Objective: The aim of this study was to evaluate the diagnostic value of FAS and B7-H4 in severe preeclampsia (sPE) and its predictive value for adverse perinatal cardiovascular outcomes. Methods: The GSE2347219 dataset was analyzed using the GEO2R online tool. Sixty healthy pregnant women and sixty patients who were sPE were collected and serum was collected. The expression levels of serum B7-H4 and FAS were analyzed by RT-qPCR. The diagnostic value of FAS and B7-H4 for sPE and the predictive value of adverse cardiovascular outcomes were assessed by direct ROC analysis. Predictive probability values were analyzed by binary logistics regression followed by ROC analysis to assess the diagnostic value and predictive value of adverse cardiovascular outcomes of combined FAS and B7-H4. Cardiomyocytes were cultured using sPE serum, and hypertrophic and fibrotic factors were detected in the cells. Results: Analysis of GSE2347219 dataset showed that FAS and B7-H4 were upregulated in sPE placental tissues. Serum assay results indicated that FAS and B7-H4 were upregulated in sPE serum. Direct ROC analysis showed that FAS and B7-H4 could distinguish sPE cases from normal pregnant women and that they could predict adverse cardiovascular outcomes. Binary logistic regression analysis and ROC analysis showed that B7-H4 in combination with FAS could effectively differentiate sPE cases from normal pregnant women and effectively predict adverse cardiovascular outcomes. sPE serum culture elevated the levels of hypertrophic factors (ANP, BNP, and β-MHC) and fibrosis factor COL1A1 in cardiomyocytes. Conclusion: B7-H4 (VTCN1) in combination with FAS can be used as a diagnostic factor and predictor of adverse cardiovascular outcomes in patients with sPE.展开更多
基金supported by the Fundamental Research Funds for the Central Universities (WUT:2020IB029)。
文摘Objective To investigate the effects of the B7-H4 gene rs10754339 and miR-125a gene rs12976445 on cancer susceptibility through a case-control study and meta-analysis.Methods A total of 1,490 cancer patients(lung/gastric/liver/:550/460/480)and 800 controls were recruited in this case-control study.The meta-analysis was performed by pooling the data from previous related studies and the present study.Results The results of this study showed that in the Hubei Han Chinese population,the rs10754339gene was significantly associated with the risk of lung and gastric cancer but not liver cancer,and the rs12976445 gene was significantly associated with the risk of lung cancer but not liver or gastric cancer.The meta-analysis results indicated that rs10754339 and rs12976445 contributed to cancer susceptibility in the Chinese population and also revealed a significant association between rs10754339and breast cancer risk,as well as between rs12976445 and lung cancer risk.Conclusion The B7-H4 gene rs10754339 and miR-125a gene rs12976445 may be the potential genetic markers for cancer susceptibility in the Chinese population,which should be validated in future studies with larger sample sizes in other ethnic populations.
文摘目的:本研究旨在评估FAS和B7-H4在重度子痫前期(sPE)中的诊断价值及其对围产期不良心血管结局的预测价值。方法:使用GEO2R在线工具分析GSE2347219数据集。收集60例健康孕妇和60名sPE患者,并收集血清。通过RT-qPCR分析血清B7-H4和FAS的表达水平。通过直接ROC分析评估FAS和B7-H4分别对sPE的诊断价值和不良心血管结局的预测价值。通过二元logistics回归分析因子预测概率值,然后进行ROC分析,评估AS和B7-H4联合对sPE的诊断价值和不良心血管结局的预测价值。使用sPE血清培养心肌细胞,检测细胞中的肥大因子和纤维化因子。结果:GSE2347219数据集分析结果显示,FAS和B7-H4在sPE胎盘组织中上调。血清检测结果表明,FAS和B7-H4在sPE血清中上调。直接ROC分析结果表明,FAS和B7-H4可以区分sPE病例与正常孕妇,而且可以预测不良心血管结局。二元logistics回归分析和ROC分析结果表明,B7-H4联合FAS可以有效地区分sPE病例与正常孕妇并有效预测不良心血管结局。sPE血清培养可以升高心肌细胞中的肥大因子(ANP、BNP和β-MHC)和纤维化因子COL1A1的水平。结论:B7-H4 (VTCN1)联合FAS可以作为sPE患者的诊断因子和不良心血管结局的预测因子。Objective: The aim of this study was to evaluate the diagnostic value of FAS and B7-H4 in severe preeclampsia (sPE) and its predictive value for adverse perinatal cardiovascular outcomes. Methods: The GSE2347219 dataset was analyzed using the GEO2R online tool. Sixty healthy pregnant women and sixty patients who were sPE were collected and serum was collected. The expression levels of serum B7-H4 and FAS were analyzed by RT-qPCR. The diagnostic value of FAS and B7-H4 for sPE and the predictive value of adverse cardiovascular outcomes were assessed by direct ROC analysis. Predictive probability values were analyzed by binary logistics regression followed by ROC analysis to assess the diagnostic value and predictive value of adverse cardiovascular outcomes of combined FAS and B7-H4. Cardiomyocytes were cultured using sPE serum, and hypertrophic and fibrotic factors were detected in the cells. Results: Analysis of GSE2347219 dataset showed that FAS and B7-H4 were upregulated in sPE placental tissues. Serum assay results indicated that FAS and B7-H4 were upregulated in sPE serum. Direct ROC analysis showed that FAS and B7-H4 could distinguish sPE cases from normal pregnant women and that they could predict adverse cardiovascular outcomes. Binary logistic regression analysis and ROC analysis showed that B7-H4 in combination with FAS could effectively differentiate sPE cases from normal pregnant women and effectively predict adverse cardiovascular outcomes. sPE serum culture elevated the levels of hypertrophic factors (ANP, BNP, and β-MHC) and fibrosis factor COL1A1 in cardiomyocytes. Conclusion: B7-H4 (VTCN1) in combination with FAS can be used as a diagnostic factor and predictor of adverse cardiovascular outcomes in patients with sPE.
