AIM: To observe the status of tumor-associated B7molecule mRNA expression in human colorectal cancer tissue by in situ hybridization.METHODS: The mRNA expression patterns of cancerassociated B7-1, B7H1, B7H2, ICOS in ...AIM: To observe the status of tumor-associated B7molecule mRNA expression in human colorectal cancer tissue by in situ hybridization.METHODS: The mRNA expression patterns of cancerassociated B7-1, B7H1, B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization (ISH) with digoxin-labeled oligonucleotide probes.RESULTS: B7-1, B7H1,B7H2, ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes (TIL).The mRNA expression level of these molecules in tumor cells was higher than that in TIL (0.76±0.54-1.62±0.82vs 0.38±0.19-0.65±0.33, P＜0.001). There was no relationship between expression level of tested B7 family molecules and patients' sex, age, differentiation status of cancer and regional lymph node metastasis.CONCLUSION: Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1,B7H2 co-signal molecules in tumor cells and TIL.展开更多
基金Supported by the National Natural Science Foundation of China, No. 30370549
文摘AIM: To observe the status of tumor-associated B7molecule mRNA expression in human colorectal cancer tissue by in situ hybridization.METHODS: The mRNA expression patterns of cancerassociated B7-1, B7H1, B7H2, ICOS in 22 specimens of human colorectal cancer tissue were monitored by in situ hybridization (ISH) with digoxin-labeled oligonucleotide probes.RESULTS: B7-1, B7H1,B7H2, ICOS mRNA were detected in both cancer cells and tumor infiltrating lymphocytes (TIL).The mRNA expression level of these molecules in tumor cells was higher than that in TIL (0.76±0.54-1.62±0.82vs 0.38±0.19-0.65±0.33, P＜0.001). There was no relationship between expression level of tested B7 family molecules and patients' sex, age, differentiation status of cancer and regional lymph node metastasis.CONCLUSION: Th2 cytokine predominant in tumor microenvironment might be related to the expression of B7H1,B7H2 co-signal molecules in tumor cells and TIL.
