INTRODUCTION There has been some previous research in the field of cardiopulmonary resuscitation (CPR) using cadaver models.[1]The Thiel method was developed by Professor Walter Thiel (Graz, Austria) and described in ...INTRODUCTION There has been some previous research in the field of cardiopulmonary resuscitation (CPR) using cadaver models.[1]The Thiel method was developed by Professor Walter Thiel (Graz, Austria) and described in 1992 and 2002. It consists of both an intravascular injection of the embalming solution and submersion of the bodies in a tank with the same solution for a determined period.[2,3]展开更多
Background: The available data on cryptogenic chronic hypersensitivity pneumonitis (ccHP) indicate an inherited predisposition to disease with triggering autoimmune phenomena. Hence, we evaluated prospectively the rol...Background: The available data on cryptogenic chronic hypersensitivity pneumonitis (ccHP) indicate an inherited predisposition to disease with triggering autoimmune phenomena. Hence, we evaluated prospectively the role of a new autoimmune regimen in treatment of its severe and progressive disease. Patients and Methods: A total of 9 patients were included in the study. They had criteria for ccHP viz. 1) clinical features of cryptogenic progressive restrictive lung disease, 2) high-resolution computed tomographic pulmonary abnormalities, and 3) bronchoalveolar lavage lymphocytosis (>30%). The regimen consisted of an initial induction phase of 3-month Solumedrol 1 g IV daily for 3 days followed by 1 month of Prednisone (P) 60 mg/day to tapered down to discontinuation by 3rd month. They also had received Mycophenolate mofetil (MMF) 1 g twice daily for 3 months. This stage was followed by a maintenance phase of yearly Rituximab infusions (1 g followed by 1 g 2 weeks later). Results: compared to their previous 6 months deterioration;all patients showed significant improvement in their forced vital volume, diffusion capacity for carbon monoxide, 6-minutes-walk after the induction phase (at 3 months) which improved further at 15 months with Rituximab therapy. Conclusion: After 3-month induction therapy with P and MMF;yearly R treatment is a safe, practical and effective long-term therapy for ccHP.展开更多
本文尝试了以 cRNA 为内在参照物,以逆转录—DNA 聚合酶链反应测定人支气管肺泡洗出液细胞肿瘤坏死因子αmRNA 含量的方法。自一重组质粒(pAW 108)体外转录出互补 RNA(cRNA),此cRNA 含两段肿瘤坏死因子α特异性引物序列。将 cRNA 用分...本文尝试了以 cRNA 为内在参照物,以逆转录—DNA 聚合酶链反应测定人支气管肺泡洗出液细胞肿瘤坏死因子αmRNA 含量的方法。自一重组质粒(pAW 108)体外转录出互补 RNA(cRNA),此cRNA 含两段肿瘤坏死因子α特异性引物序列。将 cRNA 用分光光度计定量后取一定量和提取自人支气管肺泡洗出液细胞的 mRNA 混在一起做逆转录,得到的 cDNA 混合物经1:3稀释后以肿瘤坏死因子α特异性引物做 DNA 聚合酶链反应放大.在反应中加入一定量末端标记^(32)P 的引物.放大产物经琼脂糖凝胶电泳分带,将各区带切下做β-液闪计数.以各区带的放射性计数对 cRNA 含量和细胞数做图,根据已知的 cRNA 的含量从图上可以推出一定量的细胞中的肿瘤坏死因子αmRNA 的含量。展开更多
基金SO has received a grant to fund the ventilator study.The study was funded by the Austrian Association of Emergency and Disaster Medicine(abbr.:ÖNK)with the“Reinhard Malzer Award”.The association did not interfere with any steps towards the ventilator study or the current paper.
文摘INTRODUCTION There has been some previous research in the field of cardiopulmonary resuscitation (CPR) using cadaver models.[1]The Thiel method was developed by Professor Walter Thiel (Graz, Austria) and described in 1992 and 2002. It consists of both an intravascular injection of the embalming solution and submersion of the bodies in a tank with the same solution for a determined period.[2,3]
文摘Background: The available data on cryptogenic chronic hypersensitivity pneumonitis (ccHP) indicate an inherited predisposition to disease with triggering autoimmune phenomena. Hence, we evaluated prospectively the role of a new autoimmune regimen in treatment of its severe and progressive disease. Patients and Methods: A total of 9 patients were included in the study. They had criteria for ccHP viz. 1) clinical features of cryptogenic progressive restrictive lung disease, 2) high-resolution computed tomographic pulmonary abnormalities, and 3) bronchoalveolar lavage lymphocytosis (>30%). The regimen consisted of an initial induction phase of 3-month Solumedrol 1 g IV daily for 3 days followed by 1 month of Prednisone (P) 60 mg/day to tapered down to discontinuation by 3rd month. They also had received Mycophenolate mofetil (MMF) 1 g twice daily for 3 months. This stage was followed by a maintenance phase of yearly Rituximab infusions (1 g followed by 1 g 2 weeks later). Results: compared to their previous 6 months deterioration;all patients showed significant improvement in their forced vital volume, diffusion capacity for carbon monoxide, 6-minutes-walk after the induction phase (at 3 months) which improved further at 15 months with Rituximab therapy. Conclusion: After 3-month induction therapy with P and MMF;yearly R treatment is a safe, practical and effective long-term therapy for ccHP.
文摘本文尝试了以 cRNA 为内在参照物,以逆转录—DNA 聚合酶链反应测定人支气管肺泡洗出液细胞肿瘤坏死因子αmRNA 含量的方法。自一重组质粒(pAW 108)体外转录出互补 RNA(cRNA),此cRNA 含两段肿瘤坏死因子α特异性引物序列。将 cRNA 用分光光度计定量后取一定量和提取自人支气管肺泡洗出液细胞的 mRNA 混在一起做逆转录,得到的 cDNA 混合物经1:3稀释后以肿瘤坏死因子α特异性引物做 DNA 聚合酶链反应放大.在反应中加入一定量末端标记^(32)P 的引物.放大产物经琼脂糖凝胶电泳分带,将各区带切下做β-液闪计数.以各区带的放射性计数对 cRNA 含量和细胞数做图,根据已知的 cRNA 的含量从图上可以推出一定量的细胞中的肿瘤坏死因子αmRNA 的含量。
