The blood–brain barrier(BBB) and the blood–brain tumor barrier(BBTB) prevent drug and nano-drug delivery systems from entering the brain. However, ligand-mediated nano-drug delivery systems have significantly enhanc...The blood–brain barrier(BBB) and the blood–brain tumor barrier(BBTB) prevent drug and nano-drug delivery systems from entering the brain. However, ligand-mediated nano-drug delivery systems have significantly enhanced the therapeutic treatment of glioma. In this study we investigated the mechanism especially the integrity of liposomes and lipid disks while traversing the BBB and BBTB both in vitro and in vivo. Fluorophores(DiO, DiI and DiD) were loaded into liposomes and lipid disks to form F?rster resonance energy transfer(FRET) nano-drug delivery systems. Using brain capillary endothelial cells as a BBB model, we show that liposomes and disks are present in the cytoplasm as their intact forms and traverse the BBB with a ratio of 0.68‰ and 1.67‰, respectively. Using human umbilical vein endothelial cells as BBTB model, liposomes and disks remained intact and traversed the BBTB with a ratio of 2.31‰and 8.32‰ at 3 h. Ex vivo imaging and immunohistochemical results revealed that liposomes and disks could traverse the BBB and BBTB in vivo as intact forms. In conclusion, these observations explain in part the mechanism by which nano-drug delivery systems increase the therapeutic treatment of glioma.展开更多
挥发性有机化合物(VOCs)作为具有较强毒性的污染物,对生物和环境都产生了巨大的威胁.本文通过改变N,N-二甲基甲酰胺(DMF)的添加量,制得不同比例的Bi/Bi_(2)WO_(6)(简称BBWO),通过调控Bi单质的含量制得一系列Bi/B-TiO_(2)/Bi_(2)WO_(6)(...挥发性有机化合物(VOCs)作为具有较强毒性的污染物,对生物和环境都产生了巨大的威胁.本文通过改变N,N-二甲基甲酰胺(DMF)的添加量,制得不同比例的Bi/Bi_(2)WO_(6)(简称BBWO),通过调控Bi单质的含量制得一系列Bi/B-TiO_(2)/Bi_(2)WO_(6)(简称BBTB)催化剂,并测试其光催化降解甲苯的活性.结果表明:利用5 mL DMF制备的Bi/B-TiO_(2)/Bi_(2)WO_(6)(简称5-BBTB)表现出最高的甲苯降解活性,1 h内甲苯降解效率可达85%.通过XRD、FTIR、Raman、XPS、SEM、TEM等表征技术对BBTB催化剂进行化学组成及氧空位等结构的分析,证明了Bi单质的成功析出.DRS、PL及电化学性能测试证明了Bi单质的引入可以有效地提高催化剂对于可见光的响应能力及电子-空穴的分离效率.同时,利用EPR表征分析了光催化降解甲苯的过程中产生的主要活性氧物种.Bi_(2)WO_(6)与B-TiO_(2)(N2氛围下,500℃,3 h煅烧后的黑色TiO_(2))的异质结及Bi单质的存在提高了BBTB催化剂光催化转化甲苯的效率,但较多的Bi单质的存在会成为电子和空穴重组中心,从而造成催化活性的下降.展开更多
基金supported by China Postdoctoral Science Foundation Grant (No.2017M611464)National Basic Research Program of China (973 Program, No. 2013CB932500)+2 种基金National Natural Science Foundation of China (Nos. 81773657, 81690263 and 81473149)Shanghai Education Commission Major Project (2017-01-07-00-07E00052)Shanghai International Science and Technology Cooperation Project (No. 16430723800)
文摘The blood–brain barrier(BBB) and the blood–brain tumor barrier(BBTB) prevent drug and nano-drug delivery systems from entering the brain. However, ligand-mediated nano-drug delivery systems have significantly enhanced the therapeutic treatment of glioma. In this study we investigated the mechanism especially the integrity of liposomes and lipid disks while traversing the BBB and BBTB both in vitro and in vivo. Fluorophores(DiO, DiI and DiD) were loaded into liposomes and lipid disks to form F?rster resonance energy transfer(FRET) nano-drug delivery systems. Using brain capillary endothelial cells as a BBB model, we show that liposomes and disks are present in the cytoplasm as their intact forms and traverse the BBB with a ratio of 0.68‰ and 1.67‰, respectively. Using human umbilical vein endothelial cells as BBTB model, liposomes and disks remained intact and traversed the BBTB with a ratio of 2.31‰and 8.32‰ at 3 h. Ex vivo imaging and immunohistochemical results revealed that liposomes and disks could traverse the BBB and BBTB in vivo as intact forms. In conclusion, these observations explain in part the mechanism by which nano-drug delivery systems increase the therapeutic treatment of glioma.
文摘挥发性有机化合物(VOCs)作为具有较强毒性的污染物,对生物和环境都产生了巨大的威胁.本文通过改变N,N-二甲基甲酰胺(DMF)的添加量,制得不同比例的Bi/Bi_(2)WO_(6)(简称BBWO),通过调控Bi单质的含量制得一系列Bi/B-TiO_(2)/Bi_(2)WO_(6)(简称BBTB)催化剂,并测试其光催化降解甲苯的活性.结果表明:利用5 mL DMF制备的Bi/B-TiO_(2)/Bi_(2)WO_(6)(简称5-BBTB)表现出最高的甲苯降解活性,1 h内甲苯降解效率可达85%.通过XRD、FTIR、Raman、XPS、SEM、TEM等表征技术对BBTB催化剂进行化学组成及氧空位等结构的分析,证明了Bi单质的成功析出.DRS、PL及电化学性能测试证明了Bi单质的引入可以有效地提高催化剂对于可见光的响应能力及电子-空穴的分离效率.同时,利用EPR表征分析了光催化降解甲苯的过程中产生的主要活性氧物种.Bi_(2)WO_(6)与B-TiO_(2)(N2氛围下,500℃,3 h煅烧后的黑色TiO_(2))的异质结及Bi单质的存在提高了BBTB催化剂光催化转化甲苯的效率,但较多的Bi单质的存在会成为电子和空穴重组中心,从而造成催化活性的下降.