Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus\|free plantlets are used to prevent...Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus\|free plantlets are used to prevent BBTD in banana production, therefore, it is very important to establish a method to detect BBTV quickly, sensitively and specifically. ELISA is now popularly used to detect BBTV. The sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo\|positive results often occur. According to DNA coding sequences of component Ⅲ,Ⅳ and Ⅰ of BBTV isolates from Zhangzhou, China, three pairs of primers are designed to establish a PCR method to specifically amplify parts of coding sequences of the BBTV coat protein, movement protein and replicase\|association. This method is also applicable to detect BBTV of bananas or cultured banana seedlings in other regions.展开更多
为了研究香蕉束顶病毒与香蕉寄主致病的互作分子机制,本文报道利用Make Your Own"Mate&Plate" Library System试剂盒成功构建感染BBTV香蕉叶片的cDNA文库。通过改良CTAB法提取感染BBTV香蕉叶片的总RNA,采用SMART法反转录合成双链c...为了研究香蕉束顶病毒与香蕉寄主致病的互作分子机制,本文报道利用Make Your Own"Mate&Plate" Library System试剂盒成功构建感染BBTV香蕉叶片的cDNA文库。通过改良CTAB法提取感染BBTV香蕉叶片的总RNA,采用SMART法反转录合成双链cDNA,经靳,酶切并去除短片段之后,与经同样酶切的pGADT7-SfiI载体连接,利用电转法将重组载体转化到大肠杆菌宿主细胞中,获得初级cDNA文库,最后以初级文库100万克隆为基数扩增,得到扩增文库并提取质粒。结果得到库容量大于2.0X10。的初级文库,检测表明文库cDNA插入片段长度主要分布在700—2000bp,文库重组率为87.5%。结果表明,该文库质量较好,可用于后续酵母双杂交互作蛋白筛选试验,本研究为开展病毒与寄主互作的研究奠定基础。展开更多
香蕉束顶病毒(Banana Bunchy Top Virus,BBTV)是香蕉上一种危害严重但又难以防治的病毒。目前只能通过及时铲除染病植株来控制传播,因此快速有效的病毒检测技术是防治BBTV的关键。BBTV为单链环状DNA病毒,本试验采用优化后的DNA提取方法...香蕉束顶病毒(Banana Bunchy Top Virus,BBTV)是香蕉上一种危害严重但又难以防治的病毒。目前只能通过及时铲除染病植株来控制传播,因此快速有效的病毒检测技术是防治BBTV的关键。BBTV为单链环状DNA病毒,本试验采用优化后的DNA提取方法提取感病的香蕉样品的DNA,并用特异性引物对提取产物进行PCR检测。结果显示本试验优化的方法操作方便、用时短,并且提取产物能够用于BBTV快速检测,从而为BBTV的快速检测提供新的方法。展开更多
利用PCR方法克隆香蕉束顶病毒海南分离物(Banana bunchy top virus,BBTV-HN)DNA2编码区,将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶(GST)基因下游。所得克隆pGEX-HN-B2测序结果表明,插入的DNA2片段为267bp,且表达相位和理论上...利用PCR方法克隆香蕉束顶病毒海南分离物(Banana bunchy top virus,BBTV-HN)DNA2编码区,将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶(GST)基因下游。所得克隆pGEX-HN-B2测序结果表明,插入的DNA2片段为267bp,且表达相位和理论上设计的完全一致。把此重组质粒转化到E.coli Rosetta(DE3)中,经过异丙硫代半乳糖苷(IPTG)诱导后出现约33Ku融合蛋白质表达条带。为获得特异的血清,切下目的表达条带并免疫兔子。经Western-blotting检测,获取的血清可以和表达的33Ku融合蛋白特异结合,表明已成功获得DNA2编码蛋白的血清。展开更多
The DNA component 6 of a Chinese isolate of banana bunchy top virus (BBTV) was cloned and sequenced. It is 1,081 nucleotides in length. Between the component sequences of Chinese and Australian BBTV isolates, there ar...The DNA component 6 of a Chinese isolate of banana bunchy top virus (BBTV) was cloned and sequenced. It is 1,081 nucleotides in length. Between the component sequences of Chinese and Australian BBTV isolates, there are 85.5% nucleotide sequence identity in full length, 92.5% nucleotide sequence identity in predicted open reading frame (ORF) and 94.8% amino acid sequence identity of potentially encoded proteins. The DNA component 6 is present in all BBTV infections tested, so it may have special function in transmission, infection, replication or movement of BBTV.展开更多
文摘Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus\|free plantlets are used to prevent BBTD in banana production, therefore, it is very important to establish a method to detect BBTV quickly, sensitively and specifically. ELISA is now popularly used to detect BBTV. The sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo\|positive results often occur. According to DNA coding sequences of component Ⅲ,Ⅳ and Ⅰ of BBTV isolates from Zhangzhou, China, three pairs of primers are designed to establish a PCR method to specifically amplify parts of coding sequences of the BBTV coat protein, movement protein and replicase\|association. This method is also applicable to detect BBTV of bananas or cultured banana seedlings in other regions.
文摘为了研究香蕉束顶病毒与香蕉寄主致病的互作分子机制,本文报道利用Make Your Own"Mate&Plate" Library System试剂盒成功构建感染BBTV香蕉叶片的cDNA文库。通过改良CTAB法提取感染BBTV香蕉叶片的总RNA,采用SMART法反转录合成双链cDNA,经靳,酶切并去除短片段之后,与经同样酶切的pGADT7-SfiI载体连接,利用电转法将重组载体转化到大肠杆菌宿主细胞中,获得初级cDNA文库,最后以初级文库100万克隆为基数扩增,得到扩增文库并提取质粒。结果得到库容量大于2.0X10。的初级文库,检测表明文库cDNA插入片段长度主要分布在700—2000bp,文库重组率为87.5%。结果表明,该文库质量较好,可用于后续酵母双杂交互作蛋白筛选试验,本研究为开展病毒与寄主互作的研究奠定基础。
文摘香蕉束顶病毒(Banana Bunchy Top Virus,BBTV)是香蕉上一种危害严重但又难以防治的病毒。目前只能通过及时铲除染病植株来控制传播,因此快速有效的病毒检测技术是防治BBTV的关键。BBTV为单链环状DNA病毒,本试验采用优化后的DNA提取方法提取感病的香蕉样品的DNA,并用特异性引物对提取产物进行PCR检测。结果显示本试验优化的方法操作方便、用时短,并且提取产物能够用于BBTV快速检测,从而为BBTV的快速检测提供新的方法。
文摘利用PCR方法克隆香蕉束顶病毒海南分离物(Banana bunchy top virus,BBTV-HN)DNA2编码区,将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶(GST)基因下游。所得克隆pGEX-HN-B2测序结果表明,插入的DNA2片段为267bp,且表达相位和理论上设计的完全一致。把此重组质粒转化到E.coli Rosetta(DE3)中,经过异丙硫代半乳糖苷(IPTG)诱导后出现约33Ku融合蛋白质表达条带。为获得特异的血清,切下目的表达条带并免疫兔子。经Western-blotting检测,获取的血清可以和表达的33Ku融合蛋白特异结合,表明已成功获得DNA2编码蛋白的血清。
文摘The DNA component 6 of a Chinese isolate of banana bunchy top virus (BBTV) was cloned and sequenced. It is 1,081 nucleotides in length. Between the component sequences of Chinese and Australian BBTV isolates, there are 85.5% nucleotide sequence identity in full length, 92.5% nucleotide sequence identity in predicted open reading frame (ORF) and 94.8% amino acid sequence identity of potentially encoded proteins. The DNA component 6 is present in all BBTV infections tested, so it may have special function in transmission, infection, replication or movement of BBTV.