Objective:To explore the effect of bacilli Galmette-Gurin(BCG)-polysaccharide nuceic acid on atopic dermatitis in mice and its mechanism.Methods:Forty NC/Nga mice were selected and randomly divided into Group A(model ...Objective:To explore the effect of bacilli Galmette-Gurin(BCG)-polysaccharide nuceic acid on atopic dermatitis in mice and its mechanism.Methods:Forty NC/Nga mice were selected and randomly divided into Group A(model group),Group B(dexamethasone treatment group),Group C(BCG polysaccharide nucleic acid treatment group) and Group D(control group) with 10 mice in each group.Atopic dermatitis model were constructed by applying 2,4-dinitrochlorobenzene on the skin of the mice.Mice in Group D were treated with acetone solution(100 μ L) on the foot pad and abdomen after hair removal at the age of 7 weeks.then on ear skin at the age of 8-13 weeks.For mice in A,B and C groups,100 μL of acetone solution containing 2,4-dinitrochlorobenzene was applied to the foot pad and the abdomen at the age of 7 weeks,then on ear skins at the age of8 to 13 weeks.At the age of 7-13 weeks,mice in Group A and Group D were treated with 100 μL saline(i.p.);mice were given dexamethasone(0.1 mL/kg,i.p.) every other day for 7 weeks in Group B;mice were treated with BCG polysaccharide nucleic acid(0.5 mg/kg,i.p.) every other day for7 weeks in Group C.The ear thickness was measured every week and the scratching frequency was recorded 1 times for 10 min a week.The mice were sacrificed after the last administration of drugs,IgE,IL-4,IL-10,IL-I2 and IFN- γ in the plasma were detected using ELISA,and RT-PCR method was employed to detect the concentrations of IL-4,IL-10,IL-12 and IFN- γ proteins.After IIK staining,the lesion degree of inflammation in ear tissue was observed microscopically.Results:The ear thickness and scratching frequency of Group A were significantly higher than those in group B,C and D(P<0.05),and there was no significant difference between Group B and C(P>0.05);the concentrations of IgE,IL-4 and IL-10 in the plasma and the expression of IL-4,IL-10 mRNA in the spleen tissues of Group A,B and C were all significantly higher than those of Group D(P<0.05);the concentrations of plasma IL-12 and IFN- γ,and spleen protein expression of IL-12 and IFN- γ in Group C mice were significantly higher than those of Group A(P<0.05).Histological observation showed obvious ear tissue exudation,erythema,swelling,desquamation of skin,and scabbing in Group A.Histopathology of the skin lesion also showed hyperkeratosis,focal-parakeratosis,stratum spinosum hypertrophy,mild sponge-like edema,a large number of lymphocytes along with plasma cell infiltration in dermis,angiectasis and hyperemia in Group A,while degree of ear skin lesion in Group B and D mice was significandy lighter than that of Group A.Conclusions:BCC polysaccharide nucleic acid can significandy reduce the serum IgE concentrations,increase the expression of IL-12,IFN- γ protein,correct the imbalance of Th1/Th2 in atopic dermatitis mice,and has obvious inhibitory effect on atopic dermatitis in NC/Nga mice.展开更多
目的系统评价卡介菌多糖核酸注射液联合抗组胺药治疗慢性荨麻疹的有效性和安全性。方法计算机检索Pub Med、Cochrane图书馆对照试验资料库、EMBase、中国期刊全文数据库(CNKI)、万方数据库、维普信息资源系统(VIP)中关于卡介菌多糖核酸...目的系统评价卡介菌多糖核酸注射液联合抗组胺药治疗慢性荨麻疹的有效性和安全性。方法计算机检索Pub Med、Cochrane图书馆对照试验资料库、EMBase、中国期刊全文数据库(CNKI)、万方数据库、维普信息资源系统(VIP)中关于卡介菌多糖核酸注射液联合抗组胺药治疗慢性荨麻疹有效性和安全性的文献,检索时间为1979年1月(1979年未建立的数据库以建库日为起始时间)—2014年10月。采用Rev Man 5.1统计软件对纳入文献进行系统评价。结果共纳入文献8篇,纳入患者总数为853例。Meta分析结果显示,两组有效率比较,差异有统计学意义〔OR(95%CI)=3.49(2.42,5.04),P<0.01〕;复发率比较,差异有统计学意义〔OR(95%CI)=0.22(0.12,0.41),P<0.01〕;而不良反应发生率比较,差异无统计学意义〔OR(95%CI)=0.85(0.50,1.43),P=0.53〕。结论与单纯抗组胺药治疗相比,卡介菌多糖核酸注射液与抗组胺药联合应用治疗慢性荨麻疹的有效率较高,复发率较低,且无严重不良反应,有效性和安全性均较高。展开更多
文摘Objective:To explore the effect of bacilli Galmette-Gurin(BCG)-polysaccharide nuceic acid on atopic dermatitis in mice and its mechanism.Methods:Forty NC/Nga mice were selected and randomly divided into Group A(model group),Group B(dexamethasone treatment group),Group C(BCG polysaccharide nucleic acid treatment group) and Group D(control group) with 10 mice in each group.Atopic dermatitis model were constructed by applying 2,4-dinitrochlorobenzene on the skin of the mice.Mice in Group D were treated with acetone solution(100 μ L) on the foot pad and abdomen after hair removal at the age of 7 weeks.then on ear skin at the age of 8-13 weeks.For mice in A,B and C groups,100 μL of acetone solution containing 2,4-dinitrochlorobenzene was applied to the foot pad and the abdomen at the age of 7 weeks,then on ear skins at the age of8 to 13 weeks.At the age of 7-13 weeks,mice in Group A and Group D were treated with 100 μL saline(i.p.);mice were given dexamethasone(0.1 mL/kg,i.p.) every other day for 7 weeks in Group B;mice were treated with BCG polysaccharide nucleic acid(0.5 mg/kg,i.p.) every other day for7 weeks in Group C.The ear thickness was measured every week and the scratching frequency was recorded 1 times for 10 min a week.The mice were sacrificed after the last administration of drugs,IgE,IL-4,IL-10,IL-I2 and IFN- γ in the plasma were detected using ELISA,and RT-PCR method was employed to detect the concentrations of IL-4,IL-10,IL-12 and IFN- γ proteins.After IIK staining,the lesion degree of inflammation in ear tissue was observed microscopically.Results:The ear thickness and scratching frequency of Group A were significantly higher than those in group B,C and D(P<0.05),and there was no significant difference between Group B and C(P>0.05);the concentrations of IgE,IL-4 and IL-10 in the plasma and the expression of IL-4,IL-10 mRNA in the spleen tissues of Group A,B and C were all significantly higher than those of Group D(P<0.05);the concentrations of plasma IL-12 and IFN- γ,and spleen protein expression of IL-12 and IFN- γ in Group C mice were significantly higher than those of Group A(P<0.05).Histological observation showed obvious ear tissue exudation,erythema,swelling,desquamation of skin,and scabbing in Group A.Histopathology of the skin lesion also showed hyperkeratosis,focal-parakeratosis,stratum spinosum hypertrophy,mild sponge-like edema,a large number of lymphocytes along with plasma cell infiltration in dermis,angiectasis and hyperemia in Group A,while degree of ear skin lesion in Group B and D mice was significandy lighter than that of Group A.Conclusions:BCC polysaccharide nucleic acid can significandy reduce the serum IgE concentrations,increase the expression of IL-12,IFN- γ protein,correct the imbalance of Th1/Th2 in atopic dermatitis mice,and has obvious inhibitory effect on atopic dermatitis in NC/Nga mice.
文摘目的系统评价卡介菌多糖核酸注射液联合抗组胺药治疗慢性荨麻疹的有效性和安全性。方法计算机检索Pub Med、Cochrane图书馆对照试验资料库、EMBase、中国期刊全文数据库(CNKI)、万方数据库、维普信息资源系统(VIP)中关于卡介菌多糖核酸注射液联合抗组胺药治疗慢性荨麻疹有效性和安全性的文献,检索时间为1979年1月(1979年未建立的数据库以建库日为起始时间)—2014年10月。采用Rev Man 5.1统计软件对纳入文献进行系统评价。结果共纳入文献8篇,纳入患者总数为853例。Meta分析结果显示,两组有效率比较,差异有统计学意义〔OR(95%CI)=3.49(2.42,5.04),P<0.01〕;复发率比较,差异有统计学意义〔OR(95%CI)=0.22(0.12,0.41),P<0.01〕;而不良反应发生率比较,差异无统计学意义〔OR(95%CI)=0.85(0.50,1.43),P=0.53〕。结论与单纯抗组胺药治疗相比,卡介菌多糖核酸注射液与抗组胺药联合应用治疗慢性荨麻疹的有效率较高,复发率较低,且无严重不良反应,有效性和安全性均较高。
