<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying...<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of <i>Legionella</i> in the environment using conventional and modern technologies. The present study aims to compare detection of <i>L. pneumophila</i> in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for <i>L. pneumophila</i> after 8 h of incubation. Also, <i>L. pneumophila</i> was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of <i>L. pneumophila</i> from water samples. It was concluded that pre-enrichment incubation allows the detection of <i>L. pneumophila</i> by PCR within a maximum of 12 h from the collection of water samples.展开更多
文摘<i>Legionella pneumophila</i> (<i>L. pneumophila</i>) is the most common causative agents for all outbreaks of Legionnaires’ disease. Prevention and control of Legionellosis requires surveying and monitoring of <i>Legionella</i> in the environment using conventional and modern technologies. The present study aims to compare detection of <i>L. pneumophila</i> in water samples using both culture and PCR techniques. A pre-enriched contaminated water sample was split into 13 subsamples. Culture and PCR tests were done from the subsamples after different intervals. The results showed a positive PCR result for <i>L. pneumophila</i> after 8 h of incubation. Also, <i>L. pneumophila</i> was detected by culture on non-selective BCYNE agar and selective GPVC agar after 5 and 6 days of incubation respectively. There was no significant difference between the non-selective BCYE- and the selective GVPC method. The PCR procedure was found more sensitive and differed significantly from the conventional selective GVPC method in isolation of <i>L. pneumophila</i> from water samples. It was concluded that pre-enrichment incubation allows the detection of <i>L. pneumophila</i> by PCR within a maximum of 12 h from the collection of water samples.
