垃圾场BFCF防渗浆材配制及吸附阻滞机理的研究

为有效解决垃圾场渗滤液的渗漏问题,用于垃圾填埋场隔离墙防渗工程,通过正交试验优选出了BFCF浆材配方（质量百分比）：膨润土22%～28%、粉煤灰17%～23%、水泥18%～24%、纤维0.06%～0.12%、纯碱0.8%～1.5%、NUF-5减水剂0.4%～0.7%,余之为水。该浆材具有良好可灌性,浆材结石率〉99.0%,其固结体28d的渗透系数、抗压强度和弹性模量分别为0.12～0.98×10-7cm/s、0.75～2.1MPa和230～350 MPa。BFCF浆材具有良好吸附阻滞性能,对磷、铵态氮等无机物阻滞率在98%以上,对CODCr、BOD5阻滞率在83.74%以上,对Hg、As、Pb、Cd等重金属离子均有很好的阻滞作用,阻滞率均在99.91%以上,满足了垃圾填埋场隔离墙对浆材渗透性及抗压强度的要求,具有广阔的市场应用前景。

BFC及BFCF型单元制动器抱轮故障的处理

介绍新乡机务段配属的HXD2C型机车BFC及BFCF型单元制动器的工作原理,分析单元制动器抱轮的故障原因,并针对该故障提出现场的应急处理办法及合理化的解决方法。

缺血-再灌注对大鼠肠道组织碱性成纤维细胞生长因子和转化生长因子β基因表达的影响

目的：研究缺血再灌注损伤致肠道内源性碱性成纤维细胞生长因子（ｂＦＧＦ）和转化生长因子β（ＴＧＦβ）基因表达的变化特征与规律，探讨这两种因子基因表达变化与肠道修复的关系。方法：利用大鼠肠系膜上动脉夹闭４５分钟与再灌注６小时与２４小时动物模型，用原位杂交与逆转录聚合酶链式反应技术检测两种因子ｍＲＮＡ表达水平、定位及时相变化。结果：在正常小肠粘膜，ｂＦＧＦ与ＴＧＦβ的ｍＲＮＡ表达均为弱阳性，主要位于小肠绒毛的固有层。缺血４５分钟，小肠粘膜ｂＦＧＦｍＲＮＡ的阳性信号消失，而ＴＧＦβｍＲＮＡ的表达量却明显增加；再灌注６小时与２４小时，两种因子ｍＲＮＡ表达量接近于伤前。结论：缺血再灌注损伤可导致肠道内源性ｂＦＧＦ与ＴＧＦβ基因表达的改变。

碱性成纤维细胞生长因子的研究进展

碱性成纤维细胞生长因子是一种多肽细胞生长因子，具有广泛的生理功能。碱性成纤维细胞生长因子的研究近年来取得了迅速发展，尤其是基因的表达及临床应用等方面。本文就该生长因子基因的克隆和表达、生物功能及临床应用研究进展作一概述。

碱性成纤维细胞生长因子（BFGF）诱变性研究

本文应用活体小鼠骨髓细胞染色体畸变（ＣＡ）试验及骨髓嗜多染红细胞（ＰＣＥ）微核（ＭＮ）试验，对碱性成纤维细胞生长因子（ＢＦＧＦ）的诱变活性进行为期ｌＷＫ、２ＷＫ及４ＷＫ的实验研究。结果表明：经ＢＦＧＦ处理的实验各组微核率与正常对照组比较无显著性差异（Ｐ＞０．０５），但是，染色体畸变试验结果揭示，其与对照组比较显示出一定的诱变活性。ＣＡ及ＭＮ两种方法测定结果均无剂量效应关系。

大鼠角膜碱烧伤后碱性成纤维细胞生长因子在角膜中的表达及意义

目的 探讨大鼠角膜碱烧伤后成纤维细胞生长因子（bFGF）在角膜中的表达和意义。方法 采用碱烧伤大鼠角膜建立角膜炎症性新生血管动物模型;免疫印迹法检测大鼠角膜碱烧伤后不同时间段bFGF在角膜中的表达;免疫组织化学方法检测大鼠角膜碱烧伤后bFGF在角膜不同组织层次的表达。结果 免疫印迹法检测显示:去除上皮后的正常大鼠角膜无bFGF表达;碱烧伤后96h,大鼠角膜可检测出bFGF表达,随着时间进展,bFGF蛋白表达量逐渐增加。免疫组化结果:正常大鼠角膜上皮层表达bFGF;碱烧伤后角膜上皮层bFGF着染程度逐渐增强,在碱烧伤后48h以前,基质层浸润的炎症细胞无bFGF着染,96h以后,浸润的炎症细胞和新生血管内皮细胞均呈bFGF阳性。结论 bFGF未参与碱烧伤后角膜新生血管增殖的早期诱导,对角膜炎症性新生血管的增殖仅起到后续支持作用。

Synergistic effect of RhBMP-2 and bFGF on ectopic osteogenesis in mice

Objective:To investigate the synergistic effect and mechanism of the combined application of recombinant human bone morphogenetic protein-2(rhBMP-2) and basic fibroblast growth factor(bFGF).Methods:24 KM male mice were randomly divided into 6 groups with 4 mice in each group,namely,Group A(control group),Group B(only treated with collagen),Group C(treated with2 ng bFGF+collagen).Group D(treated with 4μg rhBMP-2+collagen),Group E(treated with 4 μg rhBMP-2+2 ng bFGF+collagen) and Group F(treated with 4 μg rhBMP-2+4 ng bFGF+collagem.The composites were implanted into the intermuscular septum of hind legs mice:whereas in control group,intermuscular septum of mice was separated and no implantation was performed.General observation,detection of concentration of calcium content,micro computed tomography(Micro-CT).three-dimensional reconstruction scan.measurement of bone mineral density(BMD).bone volume fraction(BVF) and trabecular thickness(Tb.Th).as well as histological observation with HE staining and ALP and CD34 immumohistochemical staining were performed.Results:Ectopic osteogenesis was found in Groups D.F and F mice.The difference in concentration of calcium contents was statistically significant between Groups D and E(P<0.05),but insignificant between Groups E and F(P>0.05).Micro-CT and three-dimensional reconstruction revealed continuous newborn bone substance in external surface of ectopic bone formation,and the center of bone formation did not show obvious substantial filling by bone substance.The differences in BMD,BVF and Tb.Th were statistically significant between Groups D and E or F(P<0.01 or <0.05).HE staining showed that in Groups D.E and F.newborn bone substance was mainly located at the edge of ectopic bone formation,and the bone formation in Groups E and F was better than that in Group D.ALP and CD34 immumohistochemical staining revealed the positive expression mainly at the edge of ectopic bone formation,and area of positive expression in Groups E and F was larger than that in Groups D.Conclusions:rhBMP-2 possesses the capacity to induce ectopic osteogenesis independently,but bFGF does not have this ability;the combined application of rhBMP-2 and bFGF can enhance the synergetic effect on inducing ectopic osteogenesis.

浅谈高校“四信”教育

当代大学生是社会主义建设事业的主力军和接班人、帮助他们树立正确的世界观、人生观、价值观、对促进社会主义建设事业有积极作用。本文从教育者的角度出发,提出了对受教育者实施“四信”教育的必然性。并且详细分析了这种教育的特点,以及“四信”本身所具有的内在的辩证统一关系。

bFGF反义寡核苷酸诱导胶质瘤细胞的凋亡

目的:探讨碱性成纤维细胞生长因子(bFGF)反义寡核苷酸对胶质瘤细胞凋亡的诱导作用。方法:将硫代磷酸修饰的bFGF反义寡核苷酸转染人脑胶质瘤细胞系TJ905,采用原位细胞死亡检测方法和电子显微镜,观察胶质瘤细胞凋亡的形态学变化,并用免疫组化法检测了增殖细胞核抗原(PCNA)的变化。结果:胶质瘤细胞呈现凋亡的形态改变,PCNA的表达明显降低。结论:bFGF反义寡核苷酸可诱导胶质瘤细胞的凋亡,抑制胶质瘤细胞的增殖。bFGF基因可作为反义治疗的目的基因。