Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 （ANAC092） has recently been shown to play a critical role in positively...Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 （ANAC092） has recently been shown to play a critical role in positively controlling senescence in Arabidopsis thaliana; however, no direct target gene through which it exerts its molecular function has been identified previously. Here, we report that BIFUNCTIONAL NUCLEASE1 （BFN1）, a well-known senescence-enhanced gene, is directly regulated by ORE1. We detected elevated expression of BFN1 already 2 h after induction of ORE1 in estradiol-inducible ORE1 overexpression lines and 6 h after transfection of Arabidopsis mesophyll cell protoplasts with a 35S：ORE1 construct, ORE1 and BFN1 expression patterns largely overlap, as shown by promoter-reporter gene （GUS） fusions, while BFN1 expression in senescent leaves and the abscission zones of maturing flower organs was virtually absent in ore1 mutant background. In vitro binding site assays revealed a bipartite ORE1 binding site, similar to that of ORS1, a paralog of ORE1. A bipartite ORE1 binding site was identified in the BFN1 promoter; mutating the cis-element within the context of the full-length BFN1 promoter drastically reduced OREl-mediated transactivation capacity in tran- siently transfected Arabidopsis mesophyll cell protoplasts. Furthermore, chromatin immunoprecipitation （CHIP） demon- strates in vivo binding of ORE1 to the BFN1 promoter. We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, namely SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence.展开更多
Plant senescence- or PCD-associated nucleases share significant homology with nucleases from different organisms. However, knowledge of their function is limited. Intracellular localization of the Arabidopsis senescen...Plant senescence- or PCD-associated nucleases share significant homology with nucleases from different organisms. However, knowledge of their function is limited. Intracellular localization of the Arabidopsis senescence- and PCD-associated nuclease BFN1 was investigated. Analysis of BFN1-GFP localization in transiently transformed tobacco protoplasts revealed initial localization in filamentous structures spread throughout the cytoplasm, which then clustered around the nuclei as the protoplasts senesced. These filamentous structures were identified as being of ER origin. In BFN1- GFP-transgenicArabidopsis plants, similar localization of BFN1-GFP was observed in young leaves, that is, in filamentous structures that reorganized around the nuclei only in senescing cells. In late senescence, BFN1-GFP was localized with fragmented nuclei in membrane-wrapped vesicles. BFNI＇s postulated function as a nucleic acid-degrading enzyme in senescence and PCD is supported by its localization pattern. Our results suggest the existence of a dedicated compartment mediating nucleic acid degradation in senescence and PCD processes.展开更多
Since the outbreak and spread of corona virus disease 2019(COVID-19),the prevalence of mental disorders,such as depression,has continued to increase.To explore the abnormal changes of brain functional connections in p...Since the outbreak and spread of corona virus disease 2019(COVID-19),the prevalence of mental disorders,such as depression,has continued to increase.To explore the abnormal changes of brain functional connections in patients with depression,this paper proposes a depression analysis method based on brain function network(BFN).To avoid the volume conductor effect,BFN was constructed based on phase lag index(PLI).Then the indicators closely related to depression were selected from weighted BFN based on small-worldness(SW)characteristics and binarization BFN based on the minimum spanning tree(MST).Differences analysis between groups and correlation analysis between these indicators and diagnostic indicators were performed in turn.The resting state electroencephalogram(EEG)data of 24 patients with depression and 29 healthy controls(HC)was used to verify our proposed method.The results showed that compared with HC,the information processing of BFN in patients with depression decreased,and BFN showed a trend of randomization.展开更多
The investigation of the multimode beamforming network （BFN） has been developed from its scattering matrix （S-matrix） analysis. A substrate integrated waveguide （SIW） BFN is designed and fabricated on a single R...The investigation of the multimode beamforming network （BFN） has been developed from its scattering matrix （S-matrix） analysis. A substrate integrated waveguide （SIW） BFN is designed and fabricated on a single Rogers 5880 substrate. This device is not only marked by features of conventional BFN, such as Butler matrix, but also has additional benefits, e.g. more compact configuration and higher radiation efficiency. Measured and simulated results based on the proposed structure are in a good agreement, which indicates that this novel type of BFN has good characteristics and presents an excellent candidate in the development of intelligent microwave and millimeter-wave multibeam antenna systems.展开更多
文摘Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 （ANAC092） has recently been shown to play a critical role in positively controlling senescence in Arabidopsis thaliana; however, no direct target gene through which it exerts its molecular function has been identified previously. Here, we report that BIFUNCTIONAL NUCLEASE1 （BFN1）, a well-known senescence-enhanced gene, is directly regulated by ORE1. We detected elevated expression of BFN1 already 2 h after induction of ORE1 in estradiol-inducible ORE1 overexpression lines and 6 h after transfection of Arabidopsis mesophyll cell protoplasts with a 35S：ORE1 construct, ORE1 and BFN1 expression patterns largely overlap, as shown by promoter-reporter gene （GUS） fusions, while BFN1 expression in senescent leaves and the abscission zones of maturing flower organs was virtually absent in ore1 mutant background. In vitro binding site assays revealed a bipartite ORE1 binding site, similar to that of ORS1, a paralog of ORE1. A bipartite ORE1 binding site was identified in the BFN1 promoter; mutating the cis-element within the context of the full-length BFN1 promoter drastically reduced OREl-mediated transactivation capacity in tran- siently transfected Arabidopsis mesophyll cell protoplasts. Furthermore, chromatin immunoprecipitation （CHIP） demon- strates in vivo binding of ORE1 to the BFN1 promoter. We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, namely SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence.
文摘Plant senescence- or PCD-associated nucleases share significant homology with nucleases from different organisms. However, knowledge of their function is limited. Intracellular localization of the Arabidopsis senescence- and PCD-associated nuclease BFN1 was investigated. Analysis of BFN1-GFP localization in transiently transformed tobacco protoplasts revealed initial localization in filamentous structures spread throughout the cytoplasm, which then clustered around the nuclei as the protoplasts senesced. These filamentous structures were identified as being of ER origin. In BFN1- GFP-transgenicArabidopsis plants, similar localization of BFN1-GFP was observed in young leaves, that is, in filamentous structures that reorganized around the nuclei only in senescing cells. In late senescence, BFN1-GFP was localized with fragmented nuclei in membrane-wrapped vesicles. BFNI＇s postulated function as a nucleic acid-degrading enzyme in senescence and PCD is supported by its localization pattern. Our results suggest the existence of a dedicated compartment mediating nucleic acid degradation in senescence and PCD processes.
基金supported by the National Natural Science Foundation of China(Nos.61962034,61862058)Longyuan Youth Innovation and Entrepreneurship Talent(Individual)Project and Tianyou Youth Talent Lift Program of Lanzhou Jiaotong Univesity。
文摘Since the outbreak and spread of corona virus disease 2019(COVID-19),the prevalence of mental disorders,such as depression,has continued to increase.To explore the abnormal changes of brain functional connections in patients with depression,this paper proposes a depression analysis method based on brain function network(BFN).To avoid the volume conductor effect,BFN was constructed based on phase lag index(PLI).Then the indicators closely related to depression were selected from weighted BFN based on small-worldness(SW)characteristics and binarization BFN based on the minimum spanning tree(MST).Differences analysis between groups and correlation analysis between these indicators and diagnostic indicators were performed in turn.The resting state electroencephalogram(EEG)data of 24 patients with depression and 29 healthy controls(HC)was used to verify our proposed method.The results showed that compared with HC,the information processing of BFN in patients with depression decreased,and BFN showed a trend of randomization.
基金Supported by the National Natural Science Foundation of China (Grant No. 60621002)the National High-Tech Research & Development Program of China (Grant No. 2007AA01Z2B4)the Scientific Research Foundation of Graduate School of Southeast University
文摘The investigation of the multimode beamforming network （BFN） has been developed from its scattering matrix （S-matrix） analysis. A substrate integrated waveguide （SIW） BFN is designed and fabricated on a single Rogers 5880 substrate. This device is not only marked by features of conventional BFN, such as Butler matrix, but also has additional benefits, e.g. more compact configuration and higher radiation efficiency. Measured and simulated results based on the proposed structure are in a good agreement, which indicates that this novel type of BFN has good characteristics and presents an excellent candidate in the development of intelligent microwave and millimeter-wave multibeam antenna systems.
