[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentratio...[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentrations of drug-containing serum(0.316%,0.158%and 0.079%)for 0,48,72 and 96 h,respectively.Then,the proliferation of the cells was detected with CCK-8 method,and the expression of related proteins,B lymphocytoma-2(Bcl-2),phosphorylated protein kinase B(p-Akt),protein kinase B(Akt)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH),was detected using Western blotting.[Results]The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.)Reichb.ex Meisn.var.albiflora(CH)and B.sessilicarpa Lévl.(S)(P<0.01),in dose-dependent and time-dependent manners.The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration;the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01);different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells;the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05,P<0.01),in a certain dose-dependent manner;at the same dose,the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05,P<0.01);administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05,P<0.01).[Conclusions]Obazema has the capacity to inhibit the proliferation of BGC-823 cells.The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2,and GAPDH may be the target gene of its anti-tumor mechanism.The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.展开更多
This study aimed to investigate the ability of chelerythrine chloride(CHE),the main active ingredient of Macleaya cordata,to induce apoptosis in human gastric cancer BGC-823 cells.The results demonstrate that CHE inhi...This study aimed to investigate the ability of chelerythrine chloride(CHE),the main active ingredient of Macleaya cordata,to induce apoptosis in human gastric cancer BGC-823 cells.The results demonstrate that CHE inhibits cell proliferation in a time-and dose-dependent manner with accompanying S phase arrest.It also induces apoptosis by a mechanism involving a reduction in the mitochondrial mem brane potential,the release of cytochrome c,activation of caspase 3 and cleavage of poly-ADP-ribose polymerase.In addition,CHE-induced apoptosis is accompanied by down-regulation of Bcl-x1 and Bcl-2 proteins with no change in the levels of Bax proteins.Taken together,the results support the development of CHE as a potentially useful anticancer drug for the treatment of gastric cancer.展开更多
The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. ...The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the m RNA and protein expression levels in mitochondrial pathway. S1 significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased m RNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.展开更多
AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulatedprotein 94 (grp94) in human gastric carcinoma cell line BGC-823.METHODS: The expression and localization of ...AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulatedprotein 94 (grp94) in human gastric carcinoma cell line BGC-823.METHODS: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofiuorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grpg4 and cell cycle in BGC-823 cell line.RESULTS: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9±4.94% and 79.6±5.16%, respectively. Bothof them were stained in cell plasma. There was a significant difference compared with control group (1.9±0.94%,P＜0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.CONCLUSION: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.展开更多
The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 d...The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.展开更多
目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡...目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡的DNA水平变化;RT-PCR方法检测细胞凋亡相关基因bcl-2、bax和surv iv in mRNA表达水平变化;免疫细胞化学方法检测bcl-2、bax和surv iv in蛋白表达的变化。结果经CPE作用后,人胃癌细胞BGC-823出现明显的细胞凋亡形态学变化及超微结构改变,细胞DNA琼脂糖凝胶电泳呈现梯形图。经250μg/mL CPE处理48 h后,多数BGC-823细胞被阻滞在G2/M期,而且细胞发生明显的凋亡变化,BGC-823细胞凋亡率可达18.9%。CPE可抑制BGC-823细胞bcl和surv iv in mRNA及蛋白的表达,促进baxmRNA及蛋白的表达。CPE可明显延长S180荷瘤小鼠生存期,且具有剂量依赖性。结论CPE通过阻滞BGC-823细胞于G2/M期及诱导BGC-823细胞凋亡发挥抗肿瘤作用,其作用机制与抑制细胞的bcl-2和surv iv in基因mRNA及蛋白表达、促进bax基因和蛋白的表达有关。展开更多
基金Supported by National Key Technology Research and Development Program(2018FYC1708000)Basic Research Project for Application of Science and Technology in Sichuan Province(2017JY0274)Science and Technology Research Project of Sichuan Traditional Chinese Medicine Administration(2018JC028).
文摘[Objectives]This study aimed to investigate the inhibiting effect of Obazema on proliferation of gastric cancer BGC-823 cells and its mechanism.[Methods]BGC-823 cells were treated with high,medium and low concentrations of drug-containing serum(0.316%,0.158%and 0.079%)for 0,48,72 and 96 h,respectively.Then,the proliferation of the cells was detected with CCK-8 method,and the expression of related proteins,B lymphocytoma-2(Bcl-2),phosphorylated protein kinase B(p-Akt),protein kinase B(Akt)and glyceraldehyde-3-phosphate dehydrogenase(GAPDH),was detected using Western blotting.[Results]The proliferation of the BGC-823 cells was significantly inhibited with different doses of Boenninghausenia albiflora(Hook.)Reichb.ex Meisn.var.albiflora(CH)and B.sessilicarpa Lévl.(S)(P<0.01),in dose-dependent and time-dependent manners.The inhibition of high-dose S on cell proliferation was similar to that of CTX 48 h after administration;the inhibition of high-dose CH on cell proliferation was significantly stronger than that of CTX(P<0.01);different doses of drug administration groups significantly inhibited the expression of p-Akt and Bcl-2 in the BGC-823 cells;the inhibition of high-dose CH on the expression of P-Akt and Bcl-2 and the inhibition of medium-dose CH on the expression of Bcl-2 were significantly stronger than that of CTX(P<0.05,P<0.01),in a certain dose-dependent manner;at the same dose,the inhibition of CH on the expression of the proteins was stronger than that of S(P<0.05,P<0.01);administration of S and CH significantly inhibited the expression of GAPDH compared with CTX(P<0.05,P<0.01).[Conclusions]Obazema has the capacity to inhibit the proliferation of BGC-823 cells.The mechanism may be achieved by inhibiting the expression of p-Akt and Bcl-2,and GAPDH may be the target gene of its anti-tumor mechanism.The inhibiting effect of CH on BGC-823 cells was more significantly than that of S.
基金This work was supported by grant 20080430283 from the China Postdoctoral Science Foundation and Jiangxi Yongfeng Boyuan Industry Co.,Ltd.(Jiangxi,China).
文摘This study aimed to investigate the ability of chelerythrine chloride(CHE),the main active ingredient of Macleaya cordata,to induce apoptosis in human gastric cancer BGC-823 cells.The results demonstrate that CHE inhibits cell proliferation in a time-and dose-dependent manner with accompanying S phase arrest.It also induces apoptosis by a mechanism involving a reduction in the mitochondrial mem brane potential,the release of cytochrome c,activation of caspase 3 and cleavage of poly-ADP-ribose polymerase.In addition,CHE-induced apoptosis is accompanied by down-regulation of Bcl-x1 and Bcl-2 proteins with no change in the levels of Bax proteins.Taken together,the results support the development of CHE as a potentially useful anticancer drug for the treatment of gastric cancer.
基金supported by the 11th Five Plan for Major Scientific and Technological Specialized Project for Significant Formulation of New Drugs(No.2009ZX09301-007)
文摘The aim of the study was to investigate the anti-proliferation and apoptosis-inducing effects of S1, a novel tetrandrine derivative, in human gastric cancer BGC-823 cells and explore the possible mechanism of action. The anti-proliferative activity was determined by MTT assay; the induction of cell cycle arrest and apoptosis were detected by flow cytometry. Quantitative real time RT-PCR and Western blotting were used to evaluate the m RNA and protein expression levels in mitochondrial pathway. S1 significantly reduced cell viability and induced a G2/M phase arrest and apoptosis in dose- and time-dependent manner. Further studies showed that S1 increased m RNA and protein expression of Bax and the Bax/Bcl-2 ratio. Moreover, S1 decreased the protein expression of procaspase-9 and procaspase-3, suggesting that the induction of apoptosis may be related to the alteration of the ratio of Bax/Bcl-2 and the activation of caspases. These findings suggested that S1 merits further investigation as a novel therapeutic agent for the treatment of human gastric cancer.
基金Supported by the Research Fund for Young Scholars of Beijing,No.02120031Research Program of Beijing Education Committee,No.0410025002
文摘AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulatedprotein 94 (grp94) in human gastric carcinoma cell line BGC-823.METHODS: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofiuorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grpg4 and cell cycle in BGC-823 cell line.RESULTS: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9±4.94% and 79.6±5.16%, respectively. Bothof them were stained in cell plasma. There was a significant difference compared with control group (1.9±0.94%,P＜0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823.CONCLUSION: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.
文摘The effect of LaCit on cell proliferation, DNA synthesize, cell cycle, cell membrane fluidity, growth in soft agar in human lung cancer cells PG and human gastric carcinoma cells BGC 823 was studied by MTT(3 (4,5 dimethylthiazol 2 yl) 2,5 diphenytetrazolium bromide) assay, 3 H TdR incorporation, flow cytometry, fluorescence polarization assay, and soft agar culture. The results indicate that when the concentrations of LaCit are 0.05, 0.1, 0.5 and 1 mmol·L -1 , it inhibits PG cells proliferation. When the concentrations are 0.5 and 1 mmol·L -1 LaCit inhibits PG cells 3 H TdR incorporation, and decreases the proportion of PG cells in S phase while increases the proportion of PG cells in G 1 phase and decreases the membrane fluidity of PG cells. But LaCit has no effect on BGC 823 cells except for cell membrane fluidity and no effect on 2BS cells.
文摘目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡的DNA水平变化;RT-PCR方法检测细胞凋亡相关基因bcl-2、bax和surv iv in mRNA表达水平变化;免疫细胞化学方法检测bcl-2、bax和surv iv in蛋白表达的变化。结果经CPE作用后,人胃癌细胞BGC-823出现明显的细胞凋亡形态学变化及超微结构改变,细胞DNA琼脂糖凝胶电泳呈现梯形图。经250μg/mL CPE处理48 h后,多数BGC-823细胞被阻滞在G2/M期,而且细胞发生明显的凋亡变化,BGC-823细胞凋亡率可达18.9%。CPE可抑制BGC-823细胞bcl和surv iv in mRNA及蛋白的表达,促进baxmRNA及蛋白的表达。CPE可明显延长S180荷瘤小鼠生存期,且具有剂量依赖性。结论CPE通过阻滞BGC-823细胞于G2/M期及诱导BGC-823细胞凋亡发挥抗肿瘤作用,其作用机制与抑制细胞的bcl-2和surv iv in基因mRNA及蛋白表达、促进bax基因和蛋白的表达有关。
