IM To elucidate the importance and the changing patterns of biliary concanavalin Abinding proteins (CPs) in the early stage of cholesterol gallstone.METHODS CPs concentrations and nucleation activities were measured...IM To elucidate the importance and the changing patterns of biliary concanavalin Abinding proteins (CPs) in the early stage of cholesterol gallstone.METHODS CPs concentrations and nucleation activities were measured by lectin affinity chromatography in biles of patients with cholesterol gallstone, pigment gallstone, gallbladder cholesterosis and nonbiliary diseases.RESULTS The concentrations of CPs were much higher in patients with cholesterol gallstone (039g/L±011g/L, n=36, P<001) or gallbladder cholesterosis (040g/L±009g/L, n=9, P<001) than in those with pigment gallstone (026g/L±012g/L, n=7) and/or nonbiliary diseases (027g/L±009g/L, n=10). Pronucleating activities were much stronger in patients with cholesterol gallstones (nucleation time ratio: 057±021, n=5, P<001 vs pigment gallstone and/or nonbiliary diseases) and gallbladder cholesterosis (nucleation time ratio: 044±023, n=5, P<001 vs pigment gallstone or nonbilliary diseases). The binding percentages of CPs to model biliary vesicles were also higher in patients with cholesterol gallstones (n=6) than those with pigment gallstones (n=6) (24%±09% vs 09%±05%, P<001).CONCLUSION Hypersecretion of CPs, especially those in vesicular phase may be the important changes in the early stage of cholesterol gallstone.展开更多
文摘IM To elucidate the importance and the changing patterns of biliary concanavalin Abinding proteins (CPs) in the early stage of cholesterol gallstone.METHODS CPs concentrations and nucleation activities were measured by lectin affinity chromatography in biles of patients with cholesterol gallstone, pigment gallstone, gallbladder cholesterosis and nonbiliary diseases.RESULTS The concentrations of CPs were much higher in patients with cholesterol gallstone (039g/L±011g/L, n=36, P<001) or gallbladder cholesterosis (040g/L±009g/L, n=9, P<001) than in those with pigment gallstone (026g/L±012g/L, n=7) and/or nonbiliary diseases (027g/L±009g/L, n=10). Pronucleating activities were much stronger in patients with cholesterol gallstones (nucleation time ratio: 057±021, n=5, P<001 vs pigment gallstone and/or nonbiliary diseases) and gallbladder cholesterosis (nucleation time ratio: 044±023, n=5, P<001 vs pigment gallstone or nonbilliary diseases). The binding percentages of CPs to model biliary vesicles were also higher in patients with cholesterol gallstones (n=6) than those with pigment gallstones (n=6) (24%±09% vs 09%±05%, P<001).CONCLUSION Hypersecretion of CPs, especially those in vesicular phase may be the important changes in the early stage of cholesterol gallstone.