Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplif...Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplified from HL-60 cells by RT-PCR, confirmed to be correct by sequencing and cloned into shuttle vector pAdTrack-CMV carrying a green fluorescence protein gene to generate a recombinant plasmid pAdTrack-CMV-BIMS. This plasmid and adenovirus backbone plasmid pAdEasy-1 were linearized and electroporated into E.coli BJ5183 host bacteria to mediate homologous recombination. The positive clone was identified by restrict endonuclease digestion. The recombinant pAdEasy-CMV-BIMS was transferred into HEK293 cells for packaging and amplification. The successful construction of recombinant human BIMS adenovirus （Ad-BIMS） was demonstrated by Western blot. To test whether Ad-BIMS has the capability of inducing apoptosis of tumor cells, Ad-BIMS was used to infect GC resistant Burkitt lymphoma Raji cells. Results After infected for 2-5 days, BIMS expression in Raji cells was detected by RT-PCR and Western blot. The significant growth retardation and apoptosis of Raji cells were also observed by MTI- and flow cytometry. Conclusion These results indicated that BIMS might be a potential candidate of gene therapy for chemoresistant tumor cells.展开更多
BIMS(BUSINESS INFORMATION MANAGE-MENT SYSTEM)商业信息管理系统是在国外大型商业管理软件的基础上,结合国内大、中型商业企业管理现代化的需要,应用先进的计算机及网络技术,由北京优航计算机系统有限公司(UNIMAC)研制开发的商业管理...BIMS(BUSINESS INFORMATION MANAGE-MENT SYSTEM)商业信息管理系统是在国外大型商业管理软件的基础上,结合国内大、中型商业企业管理现代化的需要,应用先进的计算机及网络技术,由北京优航计算机系统有限公司(UNIMAC)研制开发的商业管理软件包,是一个通用的模块化、集成化软件系统。 BIMS商业信息管理系统运行于UNIX系统平台,采用多窗口汉字显示,人机界面友好。展开更多
文摘Objective To transfer pro-apoptotic BIM directly into tumor cells bypass the complicated biologica processes of BIM activation so as to reverse the chemoresistance of cancer cells. Methods BIMS was specifically amplified from HL-60 cells by RT-PCR, confirmed to be correct by sequencing and cloned into shuttle vector pAdTrack-CMV carrying a green fluorescence protein gene to generate a recombinant plasmid pAdTrack-CMV-BIMS. This plasmid and adenovirus backbone plasmid pAdEasy-1 were linearized and electroporated into E.coli BJ5183 host bacteria to mediate homologous recombination. The positive clone was identified by restrict endonuclease digestion. The recombinant pAdEasy-CMV-BIMS was transferred into HEK293 cells for packaging and amplification. The successful construction of recombinant human BIMS adenovirus （Ad-BIMS） was demonstrated by Western blot. To test whether Ad-BIMS has the capability of inducing apoptosis of tumor cells, Ad-BIMS was used to infect GC resistant Burkitt lymphoma Raji cells. Results After infected for 2-5 days, BIMS expression in Raji cells was detected by RT-PCR and Western blot. The significant growth retardation and apoptosis of Raji cells were also observed by MTI- and flow cytometry. Conclusion These results indicated that BIMS might be a potential candidate of gene therapy for chemoresistant tumor cells.
文摘BIMS(BUSINESS INFORMATION MANAGE-MENT SYSTEM)商业信息管理系统是在国外大型商业管理软件的基础上,结合国内大、中型商业企业管理现代化的需要,应用先进的计算机及网络技术,由北京优航计算机系统有限公司(UNIMAC)研制开发的商业管理软件包,是一个通用的模块化、集成化软件系统。 BIMS商业信息管理系统运行于UNIX系统平台,采用多窗口汉字显示,人机界面友好。
