BACKGROUND Aeromonas species are uncommon pathogens in biliary sepsis and cause substantial mortality in patients with impaired hepatobiliary function. Asia has the highest incidence of infection from Aeromonas,wherea...BACKGROUND Aeromonas species are uncommon pathogens in biliary sepsis and cause substantial mortality in patients with impaired hepatobiliary function. Asia has the highest incidence of infection from Aeromonas,whereas cases in the west are rare.CASE SUMMARY We report the case of a 64-year-old woman with advanced pancreatic cancer and jaundice who manifested fever,abdominal pain,severe thrombocytopenia,anemia and kidney failure following the insertion of a percutaneous transhepatic biliary drainage. Blood culture results revealed the presence of Aeromonas veronii biovar veronii(A. veronii biovar veronii). After antibiotic therapy and transfusions,the life-threatening clinical conditions of the patient improved and she was discharged.CONCLUSION This was a rare case of infection,probably the first to be reported in West countries,caused by A. veronii biovar veronii following biliary drainage. A finding of Aeromonas must alert clinician to the possibility of severe sepsis.展开更多
Objectives: To characterize the distribution pattern of biovars and serotypes of Ureaplasma urealyticum in normal healthy women, sexually transmitted infections clinic clients and in sex workers. Methods: We cultured ...Objectives: To characterize the distribution pattern of biovars and serotypes of Ureaplasma urealyticum in normal healthy women, sexually transmitted infections clinic clients and in sex workers. Methods: We cultured cervical swabs taken from 261physical check-up clients, 599 STI clinic outpatients and 98sex workers using commercial selective medium. Somepositive cultures were further biotyped and serotyped byPCR. Results: (1) Biovar 1 of U. urealyticum (95.0%), especially single infection of serotype 1, 3, and 6 of biovar 1, iscommonly found in healthy women. (2) Uo urealyticum ismore commonly isolated in sex workers (90.8%) than inphysical check-up group (60.9%) and STI outpatients group(61.3%) (P< 0.001). (3) Biovar 2 infection of U. urealyticumis more prevalent in sex workers (28.1%) and STIoutpatients group (26.6%) than that in physical check-up group (4.9%) (P < 0.001). (4) Mixed infection caused bymore than one serotype of U. urealyticum is increasing fromphysical check-up group (8.6%) to STI outpatients (12.4%)and to sex workers (23.9%) (P < 0.01). (5) There is nostatistic difference in the distribution of serotype 1, 3, and 6of biovar 1 among these three groups (P=0.763). (6) ThePCR method described here is relatively simple, rapid andspecific for the biotyping and serotyping of biovar 1 of U.urealyticum. Conclusion: we should pay more attention to biovar 2and mixed infection than single infection of biovar 1 of U.urealyticum in clinic practice. PCR is a good method inbiotyping and serotyping.展开更多
Objectives: To characterize the distribution pattern ofbiovars and serotypes of Ureaplasma urealyticum in normalhealthy women, sexually transmitted infections clinic clients,and in sex workers. Methods: We cultured ce...Objectives: To characterize the distribution pattern ofbiovars and serotypes of Ureaplasma urealyticum in normalhealthy women, sexually transmitted infections clinic clients,and in sex workers. Methods: We cultured cervical swabs taken from 261physical check-up clients, 599 STI clinic outpatients and 98 sexworkers using commercial selective medium. Some positivecultures were further biotyped and serotyped by PCR. Results: (1) U. urealyticum is more commonly isolated in sexworkers (90.8%) than in the physical check-up group (60.9%)or the STI outpatient group (61.3%) (P < 0.001). (2) Biovar 1of U. urealyticum (95.0%), especially single infection ofserotype 1, 3, and 6 of biovar 1, is commonly found in healthywomen. (3) Biovar 2 infection of U. urealyticum is moreprevalent in sex workers (28.1%) and STI outpatients group(26.6%) than that in the physical check-up group (4.9%) (P <0.001). (4) Mixed infection caused by more than one serotypeof U. urealyticum increased from physical check-up group(8.6%) to STI outpatients (12.4%) to sex workers (23.9%) (P <0.01). (5) There is no statistically significant difference in thedistribution of serotype 1, 3, and 6 of biovar 1 among thesethree groups (P=0.763). (6) The PCR method described here isrelatively simple, rapid and specific for the biotyping andserotyping of biovar 1 of U. urealyticum. Conclusion: We should pay more attention to biovar 2 andmixed infections of U. urealyticum than single infection ofbiovar 1 in clinic practice. PCR is a good method for biotypingand serotyping.展开更多
Background:Brucellosis is a worldwide zoonotic disease caused by Brucella spp.Brucella invades the body through the skin mucosa,digestive tract,and respiratory tract.However,only a few studies on human spontaneous abo...Background:Brucellosis is a worldwide zoonotic disease caused by Brucella spp.Brucella invades the body through the skin mucosa,digestive tract,and respiratory tract.However,only a few studies on human spontaneous abortion attributable to Brucella have been reported.In this work,the patient living in Shanxi Province in China who had suffered a spontaneous abortion was underwent pathogen detection and Brucella melitensis biovar 3 was identified.Case presentation:The patient in this study was 22 years old.On July 16,2015,she was admitted to Shanxi Grand Hospital,Shanxi Province,China because of one day of vaginal bleeding and three days of abdominal distension accompanied by fever after five months of amenorrhea.A serum tube agglutination test for brucellosis and blood culture were positive.At the time of discharge,she was prescribed oral doxycycline(100 mg/dose,twice a day)and rifampicin(600 mg/dose,once daily)for 6 weeks as recommended by the World Health Organization(WHO).No recurrence was observed during the six months of follow-up after the cessation of antibiotic treatment.Conclusions:This is the first reported case of miscarriage resulting from Brucella melitensis biovar 3 isolated from a pregnant woman who was infected through unpasteurized milk in China.Brucellosis infection was overlooked in the Maternity Hospital because of physician unawareness.Early recognition and prompt treatment of brucellosis infection are crucial for a successful outcome in pregnancy.展开更多
文摘BACKGROUND Aeromonas species are uncommon pathogens in biliary sepsis and cause substantial mortality in patients with impaired hepatobiliary function. Asia has the highest incidence of infection from Aeromonas,whereas cases in the west are rare.CASE SUMMARY We report the case of a 64-year-old woman with advanced pancreatic cancer and jaundice who manifested fever,abdominal pain,severe thrombocytopenia,anemia and kidney failure following the insertion of a percutaneous transhepatic biliary drainage. Blood culture results revealed the presence of Aeromonas veronii biovar veronii(A. veronii biovar veronii). After antibiotic therapy and transfusions,the life-threatening clinical conditions of the patient improved and she was discharged.CONCLUSION This was a rare case of infection,probably the first to be reported in West countries,caused by A. veronii biovar veronii following biliary drainage. A finding of Aeromonas must alert clinician to the possibility of severe sepsis.
文摘Objectives: To characterize the distribution pattern of biovars and serotypes of Ureaplasma urealyticum in normal healthy women, sexually transmitted infections clinic clients and in sex workers. Methods: We cultured cervical swabs taken from 261physical check-up clients, 599 STI clinic outpatients and 98sex workers using commercial selective medium. Somepositive cultures were further biotyped and serotyped byPCR. Results: (1) Biovar 1 of U. urealyticum (95.0%), especially single infection of serotype 1, 3, and 6 of biovar 1, iscommonly found in healthy women. (2) Uo urealyticum ismore commonly isolated in sex workers (90.8%) than inphysical check-up group (60.9%) and STI outpatients group(61.3%) (P< 0.001). (3) Biovar 2 infection of U. urealyticumis more prevalent in sex workers (28.1%) and STIoutpatients group (26.6%) than that in physical check-up group (4.9%) (P < 0.001). (4) Mixed infection caused bymore than one serotype of U. urealyticum is increasing fromphysical check-up group (8.6%) to STI outpatients (12.4%)and to sex workers (23.9%) (P < 0.01). (5) There is nostatistic difference in the distribution of serotype 1, 3, and 6of biovar 1 among these three groups (P=0.763). (6) ThePCR method described here is relatively simple, rapid andspecific for the biotyping and serotyping of biovar 1 of U.urealyticum. Conclusion: we should pay more attention to biovar 2and mixed infection than single infection of biovar 1 of U.urealyticum in clinic practice. PCR is a good method inbiotyping and serotyping.
文摘Objectives: To characterize the distribution pattern ofbiovars and serotypes of Ureaplasma urealyticum in normalhealthy women, sexually transmitted infections clinic clients,and in sex workers. Methods: We cultured cervical swabs taken from 261physical check-up clients, 599 STI clinic outpatients and 98 sexworkers using commercial selective medium. Some positivecultures were further biotyped and serotyped by PCR. Results: (1) U. urealyticum is more commonly isolated in sexworkers (90.8%) than in the physical check-up group (60.9%)or the STI outpatient group (61.3%) (P < 0.001). (2) Biovar 1of U. urealyticum (95.0%), especially single infection ofserotype 1, 3, and 6 of biovar 1, is commonly found in healthywomen. (3) Biovar 2 infection of U. urealyticum is moreprevalent in sex workers (28.1%) and STI outpatients group(26.6%) than that in the physical check-up group (4.9%) (P <0.001). (4) Mixed infection caused by more than one serotypeof U. urealyticum increased from physical check-up group(8.6%) to STI outpatients (12.4%) to sex workers (23.9%) (P <0.01). (5) There is no statistically significant difference in thedistribution of serotype 1, 3, and 6 of biovar 1 among thesethree groups (P=0.763). (6) The PCR method described here isrelatively simple, rapid and specific for the biotyping andserotyping of biovar 1 of U. urealyticum. Conclusion: We should pay more attention to biovar 2 andmixed infections of U. urealyticum than single infection ofbiovar 1 in clinic practice. PCR is a good method for biotypingand serotyping.
基金This study was supported by the Science and Technology Project of the Shanxi Province Health and Family Planning Commission(No.2011077)the National Natural Science Foundation of China(No.81271900)The funders contributed to the study design and data collection.
文摘Background:Brucellosis is a worldwide zoonotic disease caused by Brucella spp.Brucella invades the body through the skin mucosa,digestive tract,and respiratory tract.However,only a few studies on human spontaneous abortion attributable to Brucella have been reported.In this work,the patient living in Shanxi Province in China who had suffered a spontaneous abortion was underwent pathogen detection and Brucella melitensis biovar 3 was identified.Case presentation:The patient in this study was 22 years old.On July 16,2015,she was admitted to Shanxi Grand Hospital,Shanxi Province,China because of one day of vaginal bleeding and three days of abdominal distension accompanied by fever after five months of amenorrhea.A serum tube agglutination test for brucellosis and blood culture were positive.At the time of discharge,she was prescribed oral doxycycline(100 mg/dose,twice a day)and rifampicin(600 mg/dose,once daily)for 6 weeks as recommended by the World Health Organization(WHO).No recurrence was observed during the six months of follow-up after the cessation of antibiotic treatment.Conclusions:This is the first reported case of miscarriage resulting from Brucella melitensis biovar 3 isolated from a pregnant woman who was infected through unpasteurized milk in China.Brucellosis infection was overlooked in the Maternity Hospital because of physician unawareness.Early recognition and prompt treatment of brucellosis infection are crucial for a successful outcome in pregnancy.
