Objective:To observe and compare the clinical effects of different electroacupuncture waveforms on primary dysmenorrhea.Methods: This was a prospective,randomized,three-group,parallel-controlled trial.Participants wit...Objective:To observe and compare the clinical effects of different electroacupuncture waveforms on primary dysmenorrhea.Methods: This was a prospective,randomized,three-group,parallel-controlled trial.Participants with primary dysmenorrhea were randomly divided into dense-sparse wave,continuous wave,and discontinuous wave groups in a 1:1:1 ratio.Two lateral Ciliao(BL 32)points were used.All three groups started treatment 3–5 days before menstruation,once a day for six sessions per course of treatment,one course of treatment per menstrual cycle,and three menstrual cycles.The primary outcome measure was the proportion with an average visual analog scale(VAS)score reduction of≥50%from baseline for dysmenorrhea in the third menstrual cycle during treatment.The secondary outcome measures included changes in dysmenorrhea VAS scores,Cox Menstrual Symptom Scale scores and the proportion of patients taking analgesic drugs.Results: The proportion of cases where the average VAS score for dysmenorrhea decreased by≥50%from baseline in the third menstrual cycle was not statistically significant(P>.05).Precisely 30 min after acupuncture and regarding immediate analgesia on the most severe day of dysmenorrhea,there was a statistically significant difference in the dense-sparse wave group compared with the other two groups during the third menstrual cycle(P<.05).Additionally,there was a statistically significant difference between the dense-sparse wave and discontinuous wave groups 24 h after acupuncture(P<.05).Conclusions: Waveform electroacupuncture can alleviate primary dysmenorrhea and its related symptoms in patients.The three groups showed similar results in terms of short-and long-term analgesic efficacy and a reduction in the number of patients taking analgesic drugs.Regarding achieving immediate analgesia,the dense-sparse wave group was slightly better than the other two groups.展开更多
[Objective] The paper was to improve the preparation efficacy of Taq DNA polymerase. [Method] Ni column was used to purify Taq DNA polymerase carrying with 6xHis tag,and recombined vector. Using the thermal-resistant ...[Objective] The paper was to improve the preparation efficacy of Taq DNA polymerase. [Method] Ni column was used to purify Taq DNA polymerase carrying with 6xHis tag,and recombined vector. Using the thermal-resistant characteristics of Taq DNA polymerase,the crude extract was treated at 75 ℃ for 1 h,and the activity of prepared enzyme solution was verified by PCR test. [Result] The recombinant pET-32A-Taq could highly express in BL21 （DE3） host bacteria and remove hybrid protein by thermal denaturation. The enzyme preparation with the activity further higher than purchased Taq DNA polymerase was obtained. [Conclusion] Taq DNA polymerase prepared by thermal purification method is simple with low cost,and can meet the needs of a large number of conventional PCR amplification.展开更多
基金supported by Technology Innovation Special Project of Dongzhimen Hospital affiliated to Beijing University of Chinese Medicine.
文摘Objective:To observe and compare the clinical effects of different electroacupuncture waveforms on primary dysmenorrhea.Methods: This was a prospective,randomized,three-group,parallel-controlled trial.Participants with primary dysmenorrhea were randomly divided into dense-sparse wave,continuous wave,and discontinuous wave groups in a 1:1:1 ratio.Two lateral Ciliao(BL 32)points were used.All three groups started treatment 3–5 days before menstruation,once a day for six sessions per course of treatment,one course of treatment per menstrual cycle,and three menstrual cycles.The primary outcome measure was the proportion with an average visual analog scale(VAS)score reduction of≥50%from baseline for dysmenorrhea in the third menstrual cycle during treatment.The secondary outcome measures included changes in dysmenorrhea VAS scores,Cox Menstrual Symptom Scale scores and the proportion of patients taking analgesic drugs.Results: The proportion of cases where the average VAS score for dysmenorrhea decreased by≥50%from baseline in the third menstrual cycle was not statistically significant(P>.05).Precisely 30 min after acupuncture and regarding immediate analgesia on the most severe day of dysmenorrhea,there was a statistically significant difference in the dense-sparse wave group compared with the other two groups during the third menstrual cycle(P<.05).Additionally,there was a statistically significant difference between the dense-sparse wave and discontinuous wave groups 24 h after acupuncture(P<.05).Conclusions: Waveform electroacupuncture can alleviate primary dysmenorrhea and its related symptoms in patients.The three groups showed similar results in terms of short-and long-term analgesic efficacy and a reduction in the number of patients taking analgesic drugs.Regarding achieving immediate analgesia,the dense-sparse wave group was slightly better than the other two groups.
基金Supported by National Natural Science Foundation of China (30872254)~~
文摘[Objective] The paper was to improve the preparation efficacy of Taq DNA polymerase. [Method] Ni column was used to purify Taq DNA polymerase carrying with 6xHis tag,and recombined vector. Using the thermal-resistant characteristics of Taq DNA polymerase,the crude extract was treated at 75 ℃ for 1 h,and the activity of prepared enzyme solution was verified by PCR test. [Result] The recombinant pET-32A-Taq could highly express in BL21 （DE3） host bacteria and remove hybrid protein by thermal denaturation. The enzyme preparation with the activity further higher than purchased Taq DNA polymerase was obtained. [Conclusion] Taq DNA polymerase prepared by thermal purification method is simple with low cost,and can meet the needs of a large number of conventional PCR amplification.
