Objective:The aim of this study is to assess the neural interconnection between the acupoint"Chéngshān(承山BL57)"and sciatic nerve from the peripheral nervous system(PNS)to the central nervous system(C...Objective:The aim of this study is to assess the neural interconnection between the acupoint"Chéngshān(承山BL57)"and sciatic nerve from the peripheral nervous system(PNS)to the central nervous system(CNS).Methods:SD rats were systematically examined from the hip to the hind limb along the route of sciatic nerve with regional anatomical technique,and further traced from the BL57 and the trunk of sciatic nerve by the neural tracing technique with cholera toxin subunit B(CTB)and CTB conjugated Alexa Fluor 488 and 594(CTB-AF488/594).Results:After regional anatomy,it was found that the sciatic nerve ran down from the hip to the hind limb and sequentially sent out the tibial and sural branches respectively to the deep and superficial layers of BL57.The CTB labeling associated with both BL57 and sciatic nerve distributed in the same spinal segments and definite regions,including the sensory neurons in lumber 3(L3)-lumber 6(L6)dorsal root ganglia(DRGs),transganglionic axons mainly in the L3-L6 spinal dorsal horn,Clarke’s nucleus,and gracile nucleus,as well as motor neurons mainly in the L3-L6 spinal ventral horn.However,the amount of neural labeling was significantly less in the cases of BL57 than that of sciatic nerve.Conclusions:These results indicate that there is distinct sensory and motor interconnection between the BL57 and the sciatic nerve,which may potentially serve for the active role of the BL57 playing in meliorating the disorders of sciatic nerve.展开更多
目的探究阿托伐他汀对高血糖诱导的小鼠脑缺血后出血转化(HT)的作用及机制。方法36只SPF级雄性C57BL/6小鼠随机分为假手术组、HT模型组和阿托伐他汀组,每组12只。比较各组小鼠神经功能评分、死亡率、HT发生率、HT分级评分,苏木精-伊红...目的探究阿托伐他汀对高血糖诱导的小鼠脑缺血后出血转化(HT)的作用及机制。方法36只SPF级雄性C57BL/6小鼠随机分为假手术组、HT模型组和阿托伐他汀组,每组12只。比较各组小鼠神经功能评分、死亡率、HT发生率、HT分级评分,苏木精-伊红染色观察脑组织出血情况,免疫荧光染色评估血脑屏障通透性,Western blot检测缺血半暗带脑组织免疫球蛋白G(IgG)、闭锁连接蛋白1(ZO-1)、闭合蛋白(occludin)、紧密连接蛋白5(claudin5)、基质金属蛋白酶(MMP)2和MMP-9的蛋白表达。结果与假手术组比较,HT模型组神经功能评分、死亡率、HT发生率、HT评分、IgG荧光强度、IgG、MMP-2、MMP-9蛋白表达水平显著增高,ZO-1、occludin、claudin5蛋白表达水平明显降低(P<0.01)。与HT模型组比较,阿托伐他汀组神经功能评分、死亡率、HT发生率、HT评分、IgG荧光强度及IgG、MMP-2、MMP-9蛋白表达水平显著降低[(2.73±1.19)分vs(3.91±0.94)分,16.7%vs 41.6%,58.3%vs 91.6%,(1.00±1.04)分vs(2.58±1.13)分,(504.30±105.52)a.u vs(859.91±153.28)a.u,4.55±1.40 vs 12.06±3.73,1.87±0.41 vs 2.95±0.68,1.47±0.24 vs 2.12±0.23,P<0.05,P<0.01],ZO-1、occludin、claduin5蛋白表达显著升高(1.55±0.20 vs 0.53±0.10,0.92±0.11 vs 0.35±0.07、0.58±0.04 vs 0.30±0.05,P<0.01)。结论阿托伐他汀可通过抑制MMP-2、MMP-9激活,上调ZO-1、occludin、claudin5表达,降低血脑屏障通透性,从而抑制高血糖诱导的脑缺血后HT。展开更多
基金the project of National Key R&D Program of China:2019YFC1709103National Natural Science Foundation of China:81774211,81774432,81801561:82004492the Fundamental Research Funds for the Central Public Welfare Research Institutes:ZZ13-YQ-068,ZZ201914001,ZZ202017006,ZZ202017015。
文摘Objective:The aim of this study is to assess the neural interconnection between the acupoint"Chéngshān(承山BL57)"and sciatic nerve from the peripheral nervous system(PNS)to the central nervous system(CNS).Methods:SD rats were systematically examined from the hip to the hind limb along the route of sciatic nerve with regional anatomical technique,and further traced from the BL57 and the trunk of sciatic nerve by the neural tracing technique with cholera toxin subunit B(CTB)and CTB conjugated Alexa Fluor 488 and 594(CTB-AF488/594).Results:After regional anatomy,it was found that the sciatic nerve ran down from the hip to the hind limb and sequentially sent out the tibial and sural branches respectively to the deep and superficial layers of BL57.The CTB labeling associated with both BL57 and sciatic nerve distributed in the same spinal segments and definite regions,including the sensory neurons in lumber 3(L3)-lumber 6(L6)dorsal root ganglia(DRGs),transganglionic axons mainly in the L3-L6 spinal dorsal horn,Clarke’s nucleus,and gracile nucleus,as well as motor neurons mainly in the L3-L6 spinal ventral horn.However,the amount of neural labeling was significantly less in the cases of BL57 than that of sciatic nerve.Conclusions:These results indicate that there is distinct sensory and motor interconnection between the BL57 and the sciatic nerve,which may potentially serve for the active role of the BL57 playing in meliorating the disorders of sciatic nerve.
文摘目的探究阿托伐他汀对高血糖诱导的小鼠脑缺血后出血转化(HT)的作用及机制。方法36只SPF级雄性C57BL/6小鼠随机分为假手术组、HT模型组和阿托伐他汀组,每组12只。比较各组小鼠神经功能评分、死亡率、HT发生率、HT分级评分,苏木精-伊红染色观察脑组织出血情况,免疫荧光染色评估血脑屏障通透性,Western blot检测缺血半暗带脑组织免疫球蛋白G(IgG)、闭锁连接蛋白1(ZO-1)、闭合蛋白(occludin)、紧密连接蛋白5(claudin5)、基质金属蛋白酶(MMP)2和MMP-9的蛋白表达。结果与假手术组比较,HT模型组神经功能评分、死亡率、HT发生率、HT评分、IgG荧光强度、IgG、MMP-2、MMP-9蛋白表达水平显著增高,ZO-1、occludin、claudin5蛋白表达水平明显降低(P<0.01)。与HT模型组比较,阿托伐他汀组神经功能评分、死亡率、HT发生率、HT评分、IgG荧光强度及IgG、MMP-2、MMP-9蛋白表达水平显著降低[(2.73±1.19)分vs(3.91±0.94)分,16.7%vs 41.6%,58.3%vs 91.6%,(1.00±1.04)分vs(2.58±1.13)分,(504.30±105.52)a.u vs(859.91±153.28)a.u,4.55±1.40 vs 12.06±3.73,1.87±0.41 vs 2.95±0.68,1.47±0.24 vs 2.12±0.23,P<0.05,P<0.01],ZO-1、occludin、claduin5蛋白表达显著升高(1.55±0.20 vs 0.53±0.10,0.92±0.11 vs 0.35±0.07、0.58±0.04 vs 0.30±0.05,P<0.01)。结论阿托伐他汀可通过抑制MMP-2、MMP-9激活,上调ZO-1、occludin、claudin5表达,降低血脑屏障通透性,从而抑制高血糖诱导的脑缺血后HT。
