and 3′ flanking region of ovine BLG were amplified from sheep genomic DNA according to the published whole sequence of ovine BLG and cloned to pGEM-T vector correspondently.By partially sequencing,the sequences of BL...and 3′ flanking region of ovine BLG were amplified from sheep genomic DNA according to the published whole sequence of ovine BLG and cloned to pGEM-T vector correspondently.By partially sequencing,the sequences of BLG 5′ and 3′ flanking were the same as that of publication completely.The recombinant structure used to direct exogenous gene especially to express in mammary gland was constructed by joining 4.2kb 5′ flanking with 2.1kb 3′ flanking.In order to assess the efficiency of BLG regulatory elements,green fluorescent protein (GFP) gene as a reporter was fused with BLG construct and transfected the mammary epithelial cells (TD47).Through observation under UV microscope and detection by fluorometer,it is demonstrated that the GFP has been successfully expressed in TD47 cell line.By virtue of direct observation and quantitative analysis,the BLG-GFP construct can be served as a model for the quick assessment of mammary gland expression construct.展开更多
The bistratified lobula giant type 1(BLG1) neuron is an identified looming-sensitive neuron in crab's visual brain that demonstrates special sensitivity to diving targets, or descending approaching motions. In thi...The bistratified lobula giant type 1(BLG1) neuron is an identified looming-sensitive neuron in crab's visual brain that demonstrates special sensitivity to diving targets, or descending approaching motions. In this paper, a novel neural model is proposed to shape such unique selectivity through incorporating a bio-plausible feedforward contrast inhibition synapse and a radially extending spatial enhancement distribution. Herein the synaptic connections and neuronal functions of this model are placed within a framework for matching and describing underlying biological findings. The systematic and comparative experiments have validated the proposed computational model that reconciles with the characteristics of BLG1 neurons in crab.展开更多
文摘and 3′ flanking region of ovine BLG were amplified from sheep genomic DNA according to the published whole sequence of ovine BLG and cloned to pGEM-T vector correspondently.By partially sequencing,the sequences of BLG 5′ and 3′ flanking were the same as that of publication completely.The recombinant structure used to direct exogenous gene especially to express in mammary gland was constructed by joining 4.2kb 5′ flanking with 2.1kb 3′ flanking.In order to assess the efficiency of BLG regulatory elements,green fluorescent protein (GFP) gene as a reporter was fused with BLG construct and transfected the mammary epithelial cells (TD47).Through observation under UV microscope and detection by fluorometer,it is demonstrated that the GFP has been successfully expressed in TD47 cell line.By virtue of direct observation and quantitative analysis,the BLG-GFP construct can be served as a model for the quick assessment of mammary gland expression construct.
基金supported by the National Natural Science Foundation of China (No.12031003)the European Union's Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Grant Agreement (No.778062 ULTRACEPT)。
文摘The bistratified lobula giant type 1(BLG1) neuron is an identified looming-sensitive neuron in crab's visual brain that demonstrates special sensitivity to diving targets, or descending approaching motions. In this paper, a novel neural model is proposed to shape such unique selectivity through incorporating a bio-plausible feedforward contrast inhibition synapse and a radially extending spatial enhancement distribution. Herein the synaptic connections and neuronal functions of this model are placed within a framework for matching and describing underlying biological findings. The systematic and comparative experiments have validated the proposed computational model that reconciles with the characteristics of BLG1 neurons in crab.
