滑膜组织Galectin⁃3、BMP⁃2蛋白在骨关节炎患者中的表达及临床价值

目的探究滑膜组织半乳糖凝集素3(Galectin⁃3)、骨形成蛋白⁃2(BMP⁃2)在骨关节炎(OA)患者中的表达及临床价值。方法选取本院2017年6月至2019年8月收治的OA患者126例作为观察组,另选取同期因膝关节韧带损伤或半月板损伤行关节镜诊治术患者124例作为对照组。采用免疫组化染色法检测两组滑膜组织Galectin⁃3、BMP⁃2,采用Logistic分析两者与OA发病的关系,对比不同病情程度患者滑膜组织Galectin⁃3、BMP⁃2、关节液炎症标记物[白介素⁃6(IL⁃6)、白介素⁃8(IL⁃8)、肿瘤坏死因子⁃α(TNF⁃α)]水平,采用Pearson分析滑膜组织Galectin⁃3、BMP⁃2与关节液炎症标记物相关性。结果观察组滑膜组织Galectin⁃3、BMP⁃2阳性表达率高于对照组,差异有统计学意义(P<0.05);滑膜组织Galectin⁃3、BMP⁃2与OA发病显著相关,差异有统计学意义(P<0.05);滑膜组织Galectin⁃3、BMP⁃2阳性表达率随病情程度增加呈升高趋势;关节液IL⁃6、IL⁃8、TNF⁃α水平随病情程度增加呈升高趋势,差异有统计学意义(P<0.05);滑膜组织Galectin⁃3与关节液IL⁃6(r=0.515)、IL⁃8(r=0.635)、TNF⁃α(r=0.620)呈正相关,滑膜组织BMP⁃2与关节液IL⁃6(r=0.602)、IL⁃8(r=0.499)、TNF⁃α(r=0.634)呈正相关,差异有统计学意义(P<0.05)。结论滑膜组织Galectin⁃3、BMP⁃2在OA患者中呈高表达,与OA的发病和病情显著相关,并与关节液炎症因子表达呈正相关,提示检测滑膜组织Galectin⁃3、BMP⁃2可辅助OA及其严重程度的诊断和预后的判断。

Reciprocal action between BMP-2 and BMP-3 in cultured fibroblast in vitro

Objective: To explore reciprocal action between BMP 2 (bone morphogenetic protein 2) and BMP 3 for better understanding of the mechanism of BMP during bone fracture union. Methods: rhBMP 2 was added into the cultured fibroblasts with the concentration of 1 200 ng/ml. The expression of BMP 3 in fibroblasts was detected by immunohistochemistry. Eukaryotic expression vector pcDNA3 BMP 3 was transfected into the fibroblasts. After the effective expression of BMP 3 was identified, BMP 2 was also detected by immunohistochemistry in BMP 3 expression cells. The fibroblasts transfected with empty vector pcDNA3 were used as the control. Results: Exogenous rhBMP 2 could promote the expression of BMP 3 in fibroblasts. BMP 3 also could be detected in these cells.Conclusions: BMP 2 and BMP 3 could reciprocally adjust the expression in fibroblasts.