BNC1调控食管鳞状细胞癌细胞的恶性生物学行为及其可能的作用机制

目的:探讨碱性核蛋白1(BNC1)对食管鳞状细胞癌(ESCC)细胞增殖、迁移、侵袭、细胞周期和凋亡的影响及其作用机制。方法:通过q PCR法检测ESCC细胞和正常食管上皮细胞中BNC1 m RNA的表达水平,免疫组织化学染色法检测10例ESCC患者癌及癌旁组织中BNC1的蛋白表达水平。利用si RNA敲低BNC1在KYSE-150和KYSE-30细胞中的表达,CCK-8法、划痕愈合实验、Transwell实验和流式细胞术检测BNC1对细胞增殖、迁移、侵袭、细胞周期和凋亡等的影响。通过CHIP-seq实验和GEPIA在线网站数据分析并结合敲低BNC1后的转录组测序数据分析筛选BNC1调控的下游靶基因,q PCR法验证BNC1敲低后靶基因的表达变化,并用双荧光素酶报告基因实验验证BNC1对靶基因的调控作用。结果:BNC1 m RNA和蛋白水平在ESCC组织中较癌旁组织高表达(均P<0.01)。敲低BNC1可明显抑制KYSE-150、KYSE-30细胞的增殖、迁移和侵袭能力(P<0.05或P<0.01),将细胞阻滞于G1期并促进细胞的凋亡(均P<0.01)。CHIP-seq实验结果和在线网站GEPIA数据分析结合敲低BNC1后的转录组测序数据显示,G蛋白通路抑制因子1(GPS1)可能为BNC1正向调控的致癌靶基因。q PCR法和双荧光素酶报告基因实验结果显示,BNC1对GPS1有调控作用(P<0.01)。结论:BNC1在ESCC组织和细胞中高表达,干扰BNC1可显著抑制ESCC细胞的增殖、迁移和侵袭能力,阻滞细胞于G1期并促进细胞凋亡,其机制可能是BNC1通过靶向GPS1调控ESCC细胞的恶性生物学行为。

Basonuclin 1 deficiency causes testicular premature aging: BNC1 cooperates with TAF7L to regulate spermatogenesis

Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic cells.However,its roles in spermatogenesis and testicular aging were not dear.Previously we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency pedigree.In this study,we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature aging.Genome-wide expression profiling and direct binding studies(by chromatin immunoprecipitation sequencing)with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10(Klhl1O),testis expressed 14(Tex14)9 and spermatogenesis and centriole associated 1(Spatcl).Moreover,biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like(TAF7L),a germ cell-specific paralogue of the transcription factor IID subunit TAF7,both in vitro and in testis,suggesting that BNC1 might directly cooperate with TAF7L to regulate spermatogenesis.The truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex,thus,disturbing expression of related genes and leading to testicular premature aging.Similarly,expressions of Y-box-binding protein 2(YBX2),outer dense fiber of sperm tails 1(ODfl),and glyceraldehyde-3-phosphate dehydrogenase,spermatogenic(GAPDHS)were significantly decreased in the testis of men with non-obstructive azoospermia.The present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.

低肥力下不同施肥方法对AC8328BNC_1玉米种质的耐瘠性研究

利用 8种施肥方法对 AC832 8BNC1 的耐瘠性进行鉴定。结果表明 ,在肥力下低等试验地 ,AC832 8BNC1 在施底肥和追第二次肥下 ,第一次肥不施产量为 349.0 4 kg/ 6 6 7m2 ,较追第一次肥产量为 2 78.4 3kg/ 6 6 7m2 经济有效 ,且差异显著 ;在不施底肥和追第二次肥下 ,第一次肥不追产量为 2 5 4 .4 6 kg/ 6 6 7m2 ,较追第一次肥产量为 2 4 9.4 4 kg/ 6 6 7m2 经济有效 ,但差异不显著。说明 AC832 8BNC1 在低氮、低磷胁迫下 ,仍能较好地协调源、库、流关系 ,确保其产量稳定 ,具有较强的耐低氮和低磷能力 ;同时验证了玉米第二次追肥的必要性。

J_1BNC320数控车床数控系统设计

本文以两种实用的数控系统为例,说明了数控系统的设计方法及联接关系,揭示了它们的内部构造和如何对外设进行控制,阐述了机与电是怎样结合在一起的。。 J_1BNC320数控车床主要用来加工回转体零件,这些加工工艺对数控系统的要求是:能控制主轴正、反转和急停;刀架能实现纵向、横向进给运动,并能在换刀点自动改变四个工位,以便选择刀具:能使冷却泵自动起停;当加工螺纹时,保证主轴转一转,刀架能移动一个被加工螺纹的导程Tmm;能实现直线和圆弧插补;能有超程保护等。