目的:构建BNN6@PEG-HCuSNPs纳米粒子,同时观察其小鼠体内外抗结肠癌效果。方法:水热反应构建BNN6@PEG-HCuSNPs纳米粒子,体外观察BNN6@PEG-HCuSNPs的光热、光动力以及气体治疗效果,选取CT26细胞作为研究对象,共聚焦显微镜下观察CT26细胞...目的:构建BNN6@PEG-HCuSNPs纳米粒子,同时观察其小鼠体内外抗结肠癌效果。方法:水热反应构建BNN6@PEG-HCuSNPs纳米粒子,体外观察BNN6@PEG-HCuSNPs的光热、光动力以及气体治疗效果,选取CT26细胞作为研究对象,共聚焦显微镜下观察CT26细胞对BNN6@PEG-HCuSNPs的摄取,DCFH-DA荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放ROS的影响,DAF-FMDA荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放NO的影响,BBoxiProbe荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放ONOO-的影响,Calcein-AM和PI检测BNN6@PEG-HCuSNPs对CT26细胞存活的影响。取雌性Balb/c小鼠肿瘤建模,当肿瘤体积生长到约150 mm 3后,将荷瘤小鼠随机分成6组,每组5只:对照组、Laser组、PEG-HCuSNPs组、PEG-HCuSNPs+Laser组、BNN6@PEG-HCuSNPs组、BNN6@PEG-HCuSNPs+Laser组。在治疗过程中,通过尾静脉分别在第0和3天向对应的组注入生理盐水或20 mg/kg的BNN6@PEG-HCuSNPs纳米粒子,观察各组肿瘤体积和重量,HE染色分析细胞结构、TUNEL分析细胞DNA碎片和Ki-67抗原染色。结果:透射电镜、扫描电镜、核磁氢谱显示成功合成了BNN6@PEG-HCuSNPs纳米粒子。BNN6@PEG-HCuSNPs能够被CT26细胞有效内吞,BNN6@PEG-HCuSNPs在联合NIR-Ⅰ照射之后,在细胞水平能够稳定且有效的产生ROS和NO,并且二者经过进一步氧化反应生成更具有细胞毒性的活性氮成分ONOO-。Calcein-AM和PI结果显示BNN6@PEG-HCuSNPs在NIR-Ⅰ照射下能够引起肿瘤细胞CT26的凋亡进而达到治疗作用,且不会对HUVECs细胞和CT26细胞产生明显的毒性。光声成像监测显示BNN6@PEG-HCuSNPs能聚集在肿瘤部位,BNN6@PEG-HCuSNPs联合NIR-Ⅰ所产生的光热/光动力/气体治疗对肿瘤抑制作用优于其他组。TUNEL结果显示BNN6@PEG-HCuSNPs+Laser组所引起的大量肿瘤细胞凋亡而出现较其他组更为明显的绿色荧光;Ki-67抗原染色结果显示相比于对照组、Laser组、PEG-HCuSNPs组、BNN6@PEG-HCuSNPs组和PEG-HCuSNPs+Laser组,BNN6@PEG-HCuSNPs+Laser组的中阳性核数量减少最为明显。结论:BNN6@PEG-HCuSNPs纳米粒子小鼠体内外抗结肠癌效果显著。展开更多
A higher value of the dielectric constant of h-BN makes it quite favourable material in energy storing device. The variation in dielectric constant was observed as a function of thickness. In this research work multil...A higher value of the dielectric constant of h-BN makes it quite favourable material in energy storing device. The variation in dielectric constant was observed as a function of thickness. In this research work multilayers of Hexagonal Boron Nitride (h-BN) was fabricated by using the Chemical exfoliation method. Two solvents Dimethylformamide (DMF) and Isopropyl Alcohol (IPA) were used for the exfoliation of h-BN. Successful sonication of hexagonal boron nitride led to the formation of Boron Nitride nanosheets (BNNs). The stable dispersibility of h-BN in Dimethylformamide and Isopropyl Alcohol was confirmed by UV Visible Spectroscopy, X-ray diffraction (XRD) and Scanning electron microscopy (SEM) confirm the mono crystallite structure (002) and nanoflakes like morphology of h-BN respectively. This appropriate strategy offered a feasible route to produce multilayer of hexagonal boron nitride. After the successful fabrication of h-BN multilayers its dielectric properties were calculated by using LCR meter. Profilometer revealed the variation in thickness and value of Dielectric constant was calculated by using its formula.展开更多
Modern rare isotope beam(RIB)factories will significantly enhance the production of extremely rare isotopes(ERI)at or near drip lines.As one of the most important methods employed in RIB factories,the production of ER...Modern rare isotope beam(RIB)factories will significantly enhance the production of extremely rare isotopes(ERI)at or near drip lines.As one of the most important methods employed in RIB factories,the production of ERIs in projectile fragmentation reactions should be theoretically improved to provide better guidance for experimental research.The cross-sections of ERIs produced in 140 MeV/u^(78,86)Kr/^(58,64)Ni/^(40,48)Ca+9Be projectile fragmentation reactions were predicted using the newly proposed models[i.e.,Bayesian neural network(BNN),BNN+FRACS,and FRACS,see Chin.Phys.C,46:074104(2022)]and the frequently used EPAX3 model.With a minimum cross-section of 1015 mb,the possibilities of ERIs discovery in a new facility for rare isotope beams(FRIB)are discussed.展开更多
The exact evolutionary history of any set of biological taxa is unknown, and all phylogenetic reconstructions are approximations. The problem becomes harder when one must consider a mix of vertical and lateral phyloge...The exact evolutionary history of any set of biological taxa is unknown, and all phylogenetic reconstructions are approximations. The problem becomes harder when one must consider a mix of vertical and lateral phylogenetic signals. In this paper we propose a game theoretic approach to constructing biological networks. The key hypothesis is that evolution is driven by distinct mechanisms that seek to maximize two competing objectives, taxonomic conservation and diversity. One branch of the mathematical theory of games is brought to bear. It translates this evolutionary game hypothesis into a mathematical model in two-player zero-sum games, with the zero-sum assumption conforming to one of the fundamental constraints in nature in mass and energy conservation. We demonstrate why and how a mechanistic and localized adaptation to seek out greater information for conservation and diversity may always lead to a global Nash equilibrium in phylogenetic affinity. Our game theoretic method, referred to as bioinformatic game theory, is used to construct network clusters. As an example, we applied this method to clustering of a multidomain protein family. The protein clusters identified were consistent with known protein subfamilies, indicating that this game-theoretic approach provides a new framework in biological sequence analysis, especially in studying gene-genome and domain-protein relationships.展开更多
Tri-flo cyclone,as a dense-medium separation device,is one of the most typical environmentally friendly industrial techniques in the coal washery plants.Surprisingly,no detailed investigation has been conducted to exp...Tri-flo cyclone,as a dense-medium separation device,is one of the most typical environmentally friendly industrial techniques in the coal washery plants.Surprisingly,no detailed investigation has been conducted to explore the effectiveness of tri-flo cyclone operating parameters on their representative metallurgical responses(yield and recovery).To fill this gap,this work for the first time in the coal processing sector is going to introduce a type of advanced intelligent method(boosted-neural network"BNN")which is able to linearly and nonlinearly assess multivariable correlations among all variables,rank them based on their effectiveness and model their produced responses.These assessments and modeling were considered a new concept called"Conscious Laboratory(CL)".CL can markedly decrease the number of laboratory experiments,reduce cost,save time,remove scaling up risks,expand maintaining processes,and significantly improve our knowledge about the modeled system.In this study,a robust monitoring database from the Tabas coal plant was prepared to cover various conditions for building a CL for coal tri-flo separators.Well-known machine learning methods,random forest,and support vector regression were developed to validate BNN outcomes.The comparisons indicated the accuracy and strength of BNN over the examined traditional modeling methods.In a sentence,generating a novel BNN within the CL concept can apply in various energy and coal processing areas,fill gaps in our knowledge about possible interactions,and open a new window for plants’fully automotive process.展开更多
文摘目的:构建BNN6@PEG-HCuSNPs纳米粒子,同时观察其小鼠体内外抗结肠癌效果。方法:水热反应构建BNN6@PEG-HCuSNPs纳米粒子,体外观察BNN6@PEG-HCuSNPs的光热、光动力以及气体治疗效果,选取CT26细胞作为研究对象,共聚焦显微镜下观察CT26细胞对BNN6@PEG-HCuSNPs的摄取,DCFH-DA荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放ROS的影响,DAF-FMDA荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放NO的影响,BBoxiProbe荧光探针检测BNN6@PEG-HCuSNPs对CT26细胞释放ONOO-的影响,Calcein-AM和PI检测BNN6@PEG-HCuSNPs对CT26细胞存活的影响。取雌性Balb/c小鼠肿瘤建模,当肿瘤体积生长到约150 mm 3后,将荷瘤小鼠随机分成6组,每组5只:对照组、Laser组、PEG-HCuSNPs组、PEG-HCuSNPs+Laser组、BNN6@PEG-HCuSNPs组、BNN6@PEG-HCuSNPs+Laser组。在治疗过程中,通过尾静脉分别在第0和3天向对应的组注入生理盐水或20 mg/kg的BNN6@PEG-HCuSNPs纳米粒子,观察各组肿瘤体积和重量,HE染色分析细胞结构、TUNEL分析细胞DNA碎片和Ki-67抗原染色。结果:透射电镜、扫描电镜、核磁氢谱显示成功合成了BNN6@PEG-HCuSNPs纳米粒子。BNN6@PEG-HCuSNPs能够被CT26细胞有效内吞,BNN6@PEG-HCuSNPs在联合NIR-Ⅰ照射之后,在细胞水平能够稳定且有效的产生ROS和NO,并且二者经过进一步氧化反应生成更具有细胞毒性的活性氮成分ONOO-。Calcein-AM和PI结果显示BNN6@PEG-HCuSNPs在NIR-Ⅰ照射下能够引起肿瘤细胞CT26的凋亡进而达到治疗作用,且不会对HUVECs细胞和CT26细胞产生明显的毒性。光声成像监测显示BNN6@PEG-HCuSNPs能聚集在肿瘤部位,BNN6@PEG-HCuSNPs联合NIR-Ⅰ所产生的光热/光动力/气体治疗对肿瘤抑制作用优于其他组。TUNEL结果显示BNN6@PEG-HCuSNPs+Laser组所引起的大量肿瘤细胞凋亡而出现较其他组更为明显的绿色荧光;Ki-67抗原染色结果显示相比于对照组、Laser组、PEG-HCuSNPs组、BNN6@PEG-HCuSNPs组和PEG-HCuSNPs+Laser组,BNN6@PEG-HCuSNPs+Laser组的中阳性核数量减少最为明显。结论:BNN6@PEG-HCuSNPs纳米粒子小鼠体内外抗结肠癌效果显著。
文摘A higher value of the dielectric constant of h-BN makes it quite favourable material in energy storing device. The variation in dielectric constant was observed as a function of thickness. In this research work multilayers of Hexagonal Boron Nitride (h-BN) was fabricated by using the Chemical exfoliation method. Two solvents Dimethylformamide (DMF) and Isopropyl Alcohol (IPA) were used for the exfoliation of h-BN. Successful sonication of hexagonal boron nitride led to the formation of Boron Nitride nanosheets (BNNs). The stable dispersibility of h-BN in Dimethylformamide and Isopropyl Alcohol was confirmed by UV Visible Spectroscopy, X-ray diffraction (XRD) and Scanning electron microscopy (SEM) confirm the mono crystallite structure (002) and nanoflakes like morphology of h-BN respectively. This appropriate strategy offered a feasible route to produce multilayer of hexagonal boron nitride. After the successful fabrication of h-BN multilayers its dielectric properties were calculated by using LCR meter. Profilometer revealed the variation in thickness and value of Dielectric constant was calculated by using its formula.
基金supported by the National Natural Science Foundation of China (No. 11975091)the Program for Innovative Research Team(in Science and Technology) in University of Henan Province,China(No. 21IRTSTHN011)
文摘Modern rare isotope beam(RIB)factories will significantly enhance the production of extremely rare isotopes(ERI)at or near drip lines.As one of the most important methods employed in RIB factories,the production of ERIs in projectile fragmentation reactions should be theoretically improved to provide better guidance for experimental research.The cross-sections of ERIs produced in 140 MeV/u^(78,86)Kr/^(58,64)Ni/^(40,48)Ca+9Be projectile fragmentation reactions were predicted using the newly proposed models[i.e.,Bayesian neural network(BNN),BNN+FRACS,and FRACS,see Chin.Phys.C,46:074104(2022)]and the frequently used EPAX3 model.With a minimum cross-section of 1015 mb,the possibilities of ERIs discovery in a new facility for rare isotope beams(FRIB)are discussed.
文摘The exact evolutionary history of any set of biological taxa is unknown, and all phylogenetic reconstructions are approximations. The problem becomes harder when one must consider a mix of vertical and lateral phylogenetic signals. In this paper we propose a game theoretic approach to constructing biological networks. The key hypothesis is that evolution is driven by distinct mechanisms that seek to maximize two competing objectives, taxonomic conservation and diversity. One branch of the mathematical theory of games is brought to bear. It translates this evolutionary game hypothesis into a mathematical model in two-player zero-sum games, with the zero-sum assumption conforming to one of the fundamental constraints in nature in mass and energy conservation. We demonstrate why and how a mechanistic and localized adaptation to seek out greater information for conservation and diversity may always lead to a global Nash equilibrium in phylogenetic affinity. Our game theoretic method, referred to as bioinformatic game theory, is used to construct network clusters. As an example, we applied this method to clustering of a multidomain protein family. The protein clusters identified were consistent with known protein subfamilies, indicating that this game-theoretic approach provides a new framework in biological sequence analysis, especially in studying gene-genome and domain-protein relationships.
文摘Tri-flo cyclone,as a dense-medium separation device,is one of the most typical environmentally friendly industrial techniques in the coal washery plants.Surprisingly,no detailed investigation has been conducted to explore the effectiveness of tri-flo cyclone operating parameters on their representative metallurgical responses(yield and recovery).To fill this gap,this work for the first time in the coal processing sector is going to introduce a type of advanced intelligent method(boosted-neural network"BNN")which is able to linearly and nonlinearly assess multivariable correlations among all variables,rank them based on their effectiveness and model their produced responses.These assessments and modeling were considered a new concept called"Conscious Laboratory(CL)".CL can markedly decrease the number of laboratory experiments,reduce cost,save time,remove scaling up risks,expand maintaining processes,and significantly improve our knowledge about the modeled system.In this study,a robust monitoring database from the Tabas coal plant was prepared to cover various conditions for building a CL for coal tri-flo separators.Well-known machine learning methods,random forest,and support vector regression were developed to validate BNN outcomes.The comparisons indicated the accuracy and strength of BNN over the examined traditional modeling methods.In a sentence,generating a novel BNN within the CL concept can apply in various energy and coal processing areas,fill gaps in our knowledge about possible interactions,and open a new window for plants’fully automotive process.
