目的:研究乳腺癌患者乳腺癌手术标本中乳腺癌易感基因1(breast cancer susceptibility gene1,BRCA-1)蛋白表达、原癌基因HER-2的基因扩增与蛋白表达及其与临床病理特征之间的关系。方法:采用免疫组织化学和显色原位杂交的方法,检测乳腺...目的:研究乳腺癌患者乳腺癌手术标本中乳腺癌易感基因1(breast cancer susceptibility gene1,BRCA-1)蛋白表达、原癌基因HER-2的基因扩增与蛋白表达及其与临床病理特征之间的关系。方法:采用免疫组织化学和显色原位杂交的方法,检测乳腺癌手术切除肿瘤组织中的HER-2和BRCA-1蛋白表达以及HER-2基因扩增情况,对这两个基因的表达与临床相关病理特征之间的关系进行分析。结果:BRCA-1和HER-2蛋白表达在不同组织类型中差异无统计学意义,但在不同肿瘤大小、不同组织分级和有无淋巴结转移中差异有显著性(P<0.05)。BRCA-1蛋白表达与HER-2基因扩增率呈负相关。结论:BRCA-1和HER-2蛋白表达对乳腺癌的发生发展具有一定作用,检测BRCA-1和HER-2蛋白表达在乳腺癌的早期诊断、判断生物学行为、预测预后中有重要意义。另外BRCA-1与HER-2可能存在相关性,须进一步研究。展开更多
The authors try to decide a problem of ovarian cancer resistance to platinum drugs by the way of correlation finding between platinum-resistance of tumor and presence of gene mutations in the patient.It was shown a va...The authors try to decide a problem of ovarian cancer resistance to platinum drugs by the way of correlation finding between platinum-resistance of tumor and presence of gene mutations in the patient.It was shown a variety of options for BRCA gene mutations in patients with ovarian cancer:BRCA 1(185delAG)-64.2%,BRCA 1(5382 insC)-55.7%,and BRCA 2(6174delT)-53.8%.Authors discovered a significant positive correlation between carriage of mutations in the BRCA genes 1/2 and the sensitivity of malignant ovarian tumors to chemotherapy with platinum.Mutations in these genes occurred significantly more often in patients with platinum-sensitive ovarian cancer.展开更多
A series of Olaparib derivatives was synthesized, and their structures were confirmed by 1H NMR, MS and elemental analysis. Their antitumor activities on breast cancer susceptbility gene 1/2（BRCAl/2）-deficient cance...A series of Olaparib derivatives was synthesized, and their structures were confirmed by 1H NMR, MS and elemental analysis. Their antitumor activities on breast cancer susceptbility gene 1/2（BRCAl/2）-deficient cancer cell lines including HCC1937, Capan-1 and MDA-MB-436 were evaluated. The antitumor activity of compound Olaparib-1 was better than the positive control Olaparib in BRCAl-deficient cell line HCC1937.展开更多
文摘目的:研究乳腺癌患者乳腺癌手术标本中乳腺癌易感基因1(breast cancer susceptibility gene1,BRCA-1)蛋白表达、原癌基因HER-2的基因扩增与蛋白表达及其与临床病理特征之间的关系。方法:采用免疫组织化学和显色原位杂交的方法,检测乳腺癌手术切除肿瘤组织中的HER-2和BRCA-1蛋白表达以及HER-2基因扩增情况,对这两个基因的表达与临床相关病理特征之间的关系进行分析。结果:BRCA-1和HER-2蛋白表达在不同组织类型中差异无统计学意义,但在不同肿瘤大小、不同组织分级和有无淋巴结转移中差异有显著性(P<0.05)。BRCA-1蛋白表达与HER-2基因扩增率呈负相关。结论:BRCA-1和HER-2蛋白表达对乳腺癌的发生发展具有一定作用,检测BRCA-1和HER-2蛋白表达在乳腺癌的早期诊断、判断生物学行为、预测预后中有重要意义。另外BRCA-1与HER-2可能存在相关性,须进一步研究。
文摘The authors try to decide a problem of ovarian cancer resistance to platinum drugs by the way of correlation finding between platinum-resistance of tumor and presence of gene mutations in the patient.It was shown a variety of options for BRCA gene mutations in patients with ovarian cancer:BRCA 1(185delAG)-64.2%,BRCA 1(5382 insC)-55.7%,and BRCA 2(6174delT)-53.8%.Authors discovered a significant positive correlation between carriage of mutations in the BRCA genes 1/2 and the sensitivity of malignant ovarian tumors to chemotherapy with platinum.Mutations in these genes occurred significantly more often in patients with platinum-sensitive ovarian cancer.
文摘A series of Olaparib derivatives was synthesized, and their structures were confirmed by 1H NMR, MS and elemental analysis. Their antitumor activities on breast cancer susceptbility gene 1/2（BRCAl/2）-deficient cancer cell lines including HCC1937, Capan-1 and MDA-MB-436 were evaluated. The antitumor activity of compound Olaparib-1 was better than the positive control Olaparib in BRCAl-deficient cell line HCC1937.
