Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arab...Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana.In this study,NtBRL3 was constructed as a vector and genetically transformed to obtain‘N.Tobacco K326’overexpression of NtBRL3.The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed.The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions,and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses,respectively.Transgenic tobacco NtBRL3ox demonstrated an excessive accumulation of droughtrelated metabolites,sugars such as sucrose and maltotetraose,and amino acids such as proline,compared with WT.We discovered drought-related differential genes in the root transcriptome,among which LOX6,RD22,WSD1,CCD8,and UGT were key genes which play an important role in plant response to drought stress.Our results demonstrate that NtBRL3 overexpression in K326 enhances drought resistance in transgenic tobacco.展开更多
The aim of the present study was to determine the preventive effects of the polysaccharide of Larimichthys crocea swim bladder(PLCSB) on CCl4-induced hepatic damage in ICR mice.The in vitro preventive effects of PLCSB...The aim of the present study was to determine the preventive effects of the polysaccharide of Larimichthys crocea swim bladder(PLCSB) on CCl4-induced hepatic damage in ICR mice.The in vitro preventive effects of PLCSB on CCl4-induced liver cytotoxic effect were evaluated in BRL 3A rat liver cells using the MTT assay.The serum levels of AST,ALT,and LDH in mice were determined using commercially available kits.The levels of IL-6,IL-12,TNF-α,and IFN-γ were determined using ELISA kits.The pathological analysis of hepatic tissues was performed with H and E staining,and the gene and protein expressions were determined by RT-PCR and Western blotting,respectively.PLCSB(20 μg·m L-1) could increase the growth of BRL 3A rat liver cells treated with CCl4.The serum levels of AST,ALT,and LDH were significantly decreased when the mice were treated with two doses of PLCSB,compared with the control mice(P < 0.05).PLCSB-treated groups also showed reduced levels of the serum pro-inflammatory cytokines IL-6,IL-12,TNF-α,and IFN-γ.PLCSB could decrease the liver weight,compared to the CCl4-treated control mice.The histopathology sections of liver tissues in the 100 mg·kg-1 PLCSB group indicated that the animals were recovered well from CCl4 damage,but the 50 mg·kg-1 PLCSB group showed necrosis to a more serious extent.The 100 mg·kg-1 PLCSB group showed significantly decreased mR NA and protein expression levels of NF-κB,i NOS,and COX-2,and increased expression of IκB-α compared with the CCl4-treated control group.In conclusion,PLCSB prevented from CCl4-induced hepatic damage in vivo.展开更多
基金supported by the Science and Technology Project of Guizhou Tobacco Company(2021XM04)the Creation of“Tobacco T-DNA Activation Insertion Mutant Library and Screening of Important Trait Mutants”Project of Guizhou University Talent Introduction(Guizhou University Hezi[2013]50).
文摘Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana.In this study,NtBRL3 was constructed as a vector and genetically transformed to obtain‘N.Tobacco K326’overexpression of NtBRL3.The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed.The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions,and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses,respectively.Transgenic tobacco NtBRL3ox demonstrated an excessive accumulation of droughtrelated metabolites,sugars such as sucrose and maltotetraose,and amino acids such as proline,compared with WT.We discovered drought-related differential genes in the root transcriptome,among which LOX6,RD22,WSD1,CCD8,and UGT were key genes which play an important role in plant response to drought stress.Our results demonstrate that NtBRL3 overexpression in K326 enhances drought resistance in transgenic tobacco.
基金supported by Program for Innovation Team Building at Institutions of Higher Education in Chongqing(KJTD201325)the Program for Innovative Research Team in Chongqing University of Education(No.KYC-cxtd03-20141002)
文摘The aim of the present study was to determine the preventive effects of the polysaccharide of Larimichthys crocea swim bladder(PLCSB) on CCl4-induced hepatic damage in ICR mice.The in vitro preventive effects of PLCSB on CCl4-induced liver cytotoxic effect were evaluated in BRL 3A rat liver cells using the MTT assay.The serum levels of AST,ALT,and LDH in mice were determined using commercially available kits.The levels of IL-6,IL-12,TNF-α,and IFN-γ were determined using ELISA kits.The pathological analysis of hepatic tissues was performed with H and E staining,and the gene and protein expressions were determined by RT-PCR and Western blotting,respectively.PLCSB(20 μg·m L-1) could increase the growth of BRL 3A rat liver cells treated with CCl4.The serum levels of AST,ALT,and LDH were significantly decreased when the mice were treated with two doses of PLCSB,compared with the control mice(P < 0.05).PLCSB-treated groups also showed reduced levels of the serum pro-inflammatory cytokines IL-6,IL-12,TNF-α,and IFN-γ.PLCSB could decrease the liver weight,compared to the CCl4-treated control mice.The histopathology sections of liver tissues in the 100 mg·kg-1 PLCSB group indicated that the animals were recovered well from CCl4 damage,but the 50 mg·kg-1 PLCSB group showed necrosis to a more serious extent.The 100 mg·kg-1 PLCSB group showed significantly decreased mR NA and protein expression levels of NF-κB,i NOS,and COX-2,and increased expression of IκB-α compared with the CCl4-treated control group.In conclusion,PLCSB prevented from CCl4-induced hepatic damage in vivo.
