Bursicon is a neuropeptide that regulates cuticle sclerotization （hardening and tanning） in insect via a G-protein coupled receptor. However, the signal transduction pathway downstream of the G-protein coupled recep...Bursicon is a neuropeptide that regulates cuticle sclerotization （hardening and tanning） in insect via a G-protein coupled receptor. However, the signal transduction pathway downstream of the G-protein coupled receptor is currently not well known. In our recent microarray analysis, we identified a panel of genes regulated by bursicon in Drosophila. One of the genes, Suppressor of Hairless, or Su（H）, has drawn our attention because its product acts down-stream of the bursicon receptor. In the present study, we cloned the Drosophila homolog, mdSu（H）, from the house fly Musca domestica using 3＇ and 5＇ rapid amplification of complementary DNA ends. Real-time polymerase chain reaction analysis revealed that the level ofmdSu（H） transcript is up-regulated by ~3-fold 1 h after recombinant bursicon injection, which correlates well with the cuticle sclerotization process observed in the recombinant bursicon-injected flies. We infer that Su（H） is an essential gene involved in the insect cuticle sclerotization process.展开更多
The tanning hormone,Bursicon,is a neuropeptide secreted by the insect nervous system that functions as a heterodimer composed of Burs-αand Burs-βsubunits.It plays a critical role in the processes of cuticle tanning ...The tanning hormone,Bursicon,is a neuropeptide secreted by the insect nervous system that functions as a heterodimer composed of Burs-αand Burs-βsubunits.It plays a critical role in the processes of cuticle tanning and wing expansion in insects.In this study,we successfully identified the AcBurs-αand AcBurs-βgenes in Aphis citricidus.The open reading frames of AcBurs-αand AcBurs-βwere 480 and 417 bp in length,respectively.Both AcBurs-αand AcBurs-βexhibited 11 conserved cysteine residues.AcBurs-αand AcBurs-βwere expressed during all developmental stages of A.citricidus and showed high expression levels in the winged aphids.To investigate the potential role of AcBurs-αand AcBurs-βin wing development,we employed RNA interference(RNAi)techniques.With the efficient silencing of AcBurs-α(44.90%)and AcBurs-β(52.31%),malformed wings were induced in aphids.The proportions of malformed wings were 22.50%,25.84%,and 38.34%in dsAcBurs-α-,dsAcBur-β-,and dsAcBurs-α+dsAcBur-β-treated groups,respectively.Moreover,feeding protein kinase A inhibitors(H-89)also increased the proportion of malformed wings to 30.00%.Feeding both double-stranded RNA and inhibitors(H-89)significantly downregulated the wing development-related genes nubbin,vestigial,notch and spalt major.Silence of vestigial through RNAi also led to malformed wings.Meanwhile,the exogenous application of 3 hormones that influence wing development did not affect the expression level of AcBursicon genes.These findings indicate that AcBursicon genes plays a crucial role in wing development in A.citricidus;therefore,it represents a potential molecular target for the control of this pest through RNAi-based approaches.展开更多
文摘Bursicon is a neuropeptide that regulates cuticle sclerotization （hardening and tanning） in insect via a G-protein coupled receptor. However, the signal transduction pathway downstream of the G-protein coupled receptor is currently not well known. In our recent microarray analysis, we identified a panel of genes regulated by bursicon in Drosophila. One of the genes, Suppressor of Hairless, or Su（H）, has drawn our attention because its product acts down-stream of the bursicon receptor. In the present study, we cloned the Drosophila homolog, mdSu（H）, from the house fly Musca domestica using 3＇ and 5＇ rapid amplification of complementary DNA ends. Real-time polymerase chain reaction analysis revealed that the level ofmdSu（H） transcript is up-regulated by ~3-fold 1 h after recombinant bursicon injection, which correlates well with the cuticle sclerotization process observed in the recombinant bursicon-injected flies. We infer that Su（H） is an essential gene involved in the insect cuticle sclerotization process.
基金supported by the National Natural Science Foundation of China(32272526,32302338,and 32020103010)Natural Science Foundation of Chongqing,China(CSTB2022NSCQ-MSX0750)China Agriculture Research System of the Ministry of Finance and the Ministry of Agriculture and Rural Affairs.
文摘The tanning hormone,Bursicon,is a neuropeptide secreted by the insect nervous system that functions as a heterodimer composed of Burs-αand Burs-βsubunits.It plays a critical role in the processes of cuticle tanning and wing expansion in insects.In this study,we successfully identified the AcBurs-αand AcBurs-βgenes in Aphis citricidus.The open reading frames of AcBurs-αand AcBurs-βwere 480 and 417 bp in length,respectively.Both AcBurs-αand AcBurs-βexhibited 11 conserved cysteine residues.AcBurs-αand AcBurs-βwere expressed during all developmental stages of A.citricidus and showed high expression levels in the winged aphids.To investigate the potential role of AcBurs-αand AcBurs-βin wing development,we employed RNA interference(RNAi)techniques.With the efficient silencing of AcBurs-α(44.90%)and AcBurs-β(52.31%),malformed wings were induced in aphids.The proportions of malformed wings were 22.50%,25.84%,and 38.34%in dsAcBurs-α-,dsAcBur-β-,and dsAcBurs-α+dsAcBur-β-treated groups,respectively.Moreover,feeding protein kinase A inhibitors(H-89)also increased the proportion of malformed wings to 30.00%.Feeding both double-stranded RNA and inhibitors(H-89)significantly downregulated the wing development-related genes nubbin,vestigial,notch and spalt major.Silence of vestigial through RNAi also led to malformed wings.Meanwhile,the exogenous application of 3 hormones that influence wing development did not affect the expression level of AcBursicon genes.These findings indicate that AcBursicon genes plays a crucial role in wing development in A.citricidus;therefore,it represents a potential molecular target for the control of this pest through RNAi-based approaches.
