Phenotypic assessment of breeding population is important to identify robust lines for incorporating into future breeding programs. The objective of this study was to identify potential lines from a wheat (<i>&l...Phenotypic assessment of breeding population is important to identify robust lines for incorporating into future breeding programs. The objective of this study was to identify potential lines from a wheat (<i><span style="font-family:Verdana;">Triticum</span></i><span style="font-family:Verdana;"> <i>aestivum</i></span><span style="font-family:Verdana;"> L.) population, based on their morpho-physiological traits, for improved heat tolerance. A subset of 100 lines of the double haploid (DH) population named “Buster”, developed from two successful Oklahoma wheat varieties (Billings and Duster)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> w</span><span style="font-family:Verdana;">as</span><span style="font-family:""><span style="font-family:Verdana;"> used in the study. Two experiments were conducted one in a greenhouse and the other in growth chambers. Data on plant height, tiller number, leaf number, and photosynthetic pigments were collected from the greenhouse;whereas the data on physiological parameters (leaf net photosynthesis (Pn), transpiration (T), stomatal conductance (g</span><sub><span style="font-family:Verdana;">s</span></sub><span style="font-family:Verdana;">), intercellular carbon dioxide concentration (C</span><sub><span style="font-family:Verdana;">i</span></sub><span style="font-family:Verdana;">), electron transport rate (ETR), Photosystem II efficiency (Fv</span></span><i><span style="font-family:Verdana;">'</span></i><span style="font-family:Verdana;">/Fm</span><i><span style="font-family:Verdana;">'</span></i><span style="font-family:""><span style="font-family:Verdana;">) and instantaneous water use efficiency (IWUE)) were collected from the growth chambers. Buster lines were significantly (</span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"> < 0.05) different both morphologically and physiologically. A wide range of observations among genotypes for different morphological and physiological characteristics was found. For example, the Chlorophyll A:B ratio ranged from 1.8 to 4.3, average plant height ranged from 8.4 to 13.3 cm, and the net photosynthesis under heat stress ranged from 11.29 to 25.28 μmol CO</span><sub><span style="font-family:Verdana;">2</span></sub></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">m</span><sup><span style="font-family:Verdana;">-2</span></sup></span><span style="font-family:""><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">s</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">. The differences in leaf physiological parameters were more discernible under heat stress. This study provides a piece of baseline information on morpho-physiological characteristics of Buster lines, and identified lines can be used in future breeding programs for incorporating heat stress tolerance.</span></span></span>展开更多
Objective: To compare the effectiveness of the Bug Buster kit regimen with a single treatment of over the counter pediculicides for eliminating head lice. Design: Single blind, multicentre, randomised, comparative cli...Objective: To compare the effectiveness of the Bug Buster kit regimen with a single treatment of over the counter pediculicides for eliminating head lice. Design: Single blind, multicentre, randomised, comparative clinical study. Setting: Four counties in England and one county in Scotland. Participants: 133 young people aged 2-15 years with head louse infestation; 56 were allocated to the Bug Buster kit and 70 to pediculicide treatment. Interventions: Home use of proprietary pediculicides (organophosphate or pyrethroid) or the Bug Buster kit. Main outcome measure: Presence of head lice 2-4 days after end of treatment: day 5 for the pediculicides and day 15 for the Bug Buster kit. Results: The cure rate using the Bug Buster kit was significantly greater than that for the pediculicides (57%v 13%; relative risk 4.4, 95%confi-dence interval 2.3 to 8.5). Number needed to treat for the Bug Buster kit compared with the pediculicides was 2.26. Conclusion: The Bug Buster kit was the most effective over the counter treatment for head louse infestation in the community when compared with pediculicides.展开更多
BEING a white South African didn't make Sara Blecher part of a privileged community even during, apartheid. "On my grandfather's side we are Lithuanian Jews and he was the only one who escaped while the rest of the...BEING a white South African didn't make Sara Blecher part of a privileged community even during, apartheid. "On my grandfather's side we are Lithuanian Jews and he was the only one who escaped while the rest of the family was murdered during World War Ⅱ," she said. "Even though I am white, I am on the wrong side of history."展开更多
BACKGROUND: Nattokinase (NK) is a serine protease enzyme of the subtilisin family. It exhibits a strong fibrinolytic activity. The fibrinolytic enzymes from Bacillus sp. have attracted interest as thrombolytic agen...BACKGROUND: Nattokinase (NK) is a serine protease enzyme of the subtilisin family. It exhibits a strong fibrinolytic activity. The fibrinolytic enzymes from Bacillus sp. have attracted interest as thrombolytic agents because of their efficiency in the fibrinolytic process including plasmin activation. METHODS: In the present study, VIT garden soil was collected and subjected to isolation process in order to screen for the NK production. Screening for NK enzyme was performed by radial caseinolytic assay. The production of NK enzyme was done in two different production medium for comparative studies. The NK enzyme was purified by gel permeation chromatography. The activity of the purified NK was checked by clot lysis and casein digestion assay. To investigate the structural basis of NK and fibrinogen interaction and also to identify the best binding mode, molecular dynamics and docking studies were performed. RESULTS: Based on the morphological and biochemical characterization, the isolate was identified as Bacillus sp. The overall purification fold of NK was about 3 with the specific activity of 664U/mg and 9.9% yield. Homogeneity of the purified enzyme was analyzed and confirmed by the single band obtained in SDS-PAGE. Molecular weight of the purified protease was estimated as 25 kDa. Purified NK enzyme exhibited 97% of effective clot lysis activity. The NK was docked in to the knob region of the fibrinogen at its binding site using Dock server. A total of 26 residues of fibrinogen and 29 residues of NK constitute the interface region. However, 9 residues offibrinogen (THR238, MET264, LYS266, ARG275, THR277, ALA279, ASN308, MET310, and LYS321) and 8 residues ofNK (GLY61, SER63, THR99, PHE189, LEU209, TYR217, ASN218, and MET222) are involved in intact binding. CONCLUSIONS: A significant amount of NK enzyme was obtained from Bacillus sp. The docking analysis revealed that the NK and fibrinogen adopt an extended binding pattern and interacts with the crucial residues to exhibit their activity.展开更多
BACKGROUND: Screening of isolates for their potency to produce streptokinase was an important criterion of this research. The current study emphasizes the strain improvement, optimization and purification studies for...BACKGROUND: Screening of isolates for their potency to produce streptokinase was an important criterion of this research. The current study emphasizes the strain improvement, optimization and purification studies for enhanced production of streptokinase from Streptococcus uberis TNA-M1 isolated from bovine milk. METHODS: The study was carried out on samples collected from milk sample. Primary screening and characterization is used as an excellent source for the isolation of 13-hemolytic organisms. Strain improvement was done by both physical & chemical mutagenesis. The enzyme activity was checked by clot lysis assay and confirmed by fibrin plate method. The partially purified and crude enzyme were analysed by high-performance liquid chromatography. Molecular weight & enzyme purity was checked by SDS -PAGE, further confirmed by fibrin zymography. RESULTS: Out of the 3 isolated strains, only one isolate expressed 13-haemolysis with streptokinase (SK) activity. Based on the results of radial caseinolytic assay and blood clot dissolving assay, isolate TNA-M1 demonstrated the highest streptokinase activity. Based on morphological, biochemical and molecular characterization, it was identified as Streptococcus uberis and the strain was named as Streptococcus uberis TNA-M1. The results indicated that ultra-violet (UV) and ethyl methane sulfonate (EMS) were effective mutagenic agents for strain improvement of Streptococcus uberis TNA-M1 and enhanced SK productivity. HPLC analysis was performed in order to confirm the presence of streptokinase with the similar retention time (0.875 min) with its standard (0.854) min. SDS-PAGE of the enzyme showed protein band of approximately 47 kDa and confirmed by fibrin zymography. It exhibited fibrinolytic activity, which was more potent than other fibrinolytic enzymes. Glucose and peptone were recorded to be the optimum carbon and nitrogen sources respectively. CONCLUSION: Thus this study presents its novelty by highlighting the potential of Streptococcus uberis TNA-M1 as a significant source for the production of fibrinolytic enzymes.展开更多
文摘Phenotypic assessment of breeding population is important to identify robust lines for incorporating into future breeding programs. The objective of this study was to identify potential lines from a wheat (<i><span style="font-family:Verdana;">Triticum</span></i><span style="font-family:Verdana;"> <i>aestivum</i></span><span style="font-family:Verdana;"> L.) population, based on their morpho-physiological traits, for improved heat tolerance. A subset of 100 lines of the double haploid (DH) population named “Buster”, developed from two successful Oklahoma wheat varieties (Billings and Duster)</span><span style="font-family:Verdana;">,</span><span style="font-family:Verdana;"> w</span><span style="font-family:Verdana;">as</span><span style="font-family:""><span style="font-family:Verdana;"> used in the study. Two experiments were conducted one in a greenhouse and the other in growth chambers. Data on plant height, tiller number, leaf number, and photosynthetic pigments were collected from the greenhouse;whereas the data on physiological parameters (leaf net photosynthesis (Pn), transpiration (T), stomatal conductance (g</span><sub><span style="font-family:Verdana;">s</span></sub><span style="font-family:Verdana;">), intercellular carbon dioxide concentration (C</span><sub><span style="font-family:Verdana;">i</span></sub><span style="font-family:Verdana;">), electron transport rate (ETR), Photosystem II efficiency (Fv</span></span><i><span style="font-family:Verdana;">'</span></i><span style="font-family:Verdana;">/Fm</span><i><span style="font-family:Verdana;">'</span></i><span style="font-family:""><span style="font-family:Verdana;">) and instantaneous water use efficiency (IWUE)) were collected from the growth chambers. Buster lines were significantly (</span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"> < 0.05) different both morphologically and physiologically. A wide range of observations among genotypes for different morphological and physiological characteristics was found. For example, the Chlorophyll A:B ratio ranged from 1.8 to 4.3, average plant height ranged from 8.4 to 13.3 cm, and the net photosynthesis under heat stress ranged from 11.29 to 25.28 μmol CO</span><sub><span style="font-family:Verdana;">2</span></sub></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">m</span><sup><span style="font-family:Verdana;">-2</span></sup></span><span style="font-family:""><span style="font-family:Verdana;">·</span><span><span style="font-family:Verdana;">s</span><sup><span style="font-family:Verdana;">-1</span></sup><span style="font-family:Verdana;">. The differences in leaf physiological parameters were more discernible under heat stress. This study provides a piece of baseline information on morpho-physiological characteristics of Buster lines, and identified lines can be used in future breeding programs for incorporating heat stress tolerance.</span></span></span>
文摘Objective: To compare the effectiveness of the Bug Buster kit regimen with a single treatment of over the counter pediculicides for eliminating head lice. Design: Single blind, multicentre, randomised, comparative clinical study. Setting: Four counties in England and one county in Scotland. Participants: 133 young people aged 2-15 years with head louse infestation; 56 were allocated to the Bug Buster kit and 70 to pediculicide treatment. Interventions: Home use of proprietary pediculicides (organophosphate or pyrethroid) or the Bug Buster kit. Main outcome measure: Presence of head lice 2-4 days after end of treatment: day 5 for the pediculicides and day 15 for the Bug Buster kit. Results: The cure rate using the Bug Buster kit was significantly greater than that for the pediculicides (57%v 13%; relative risk 4.4, 95%confi-dence interval 2.3 to 8.5). Number needed to treat for the Bug Buster kit compared with the pediculicides was 2.26. Conclusion: The Bug Buster kit was the most effective over the counter treatment for head louse infestation in the community when compared with pediculicides.
文摘BEING a white South African didn't make Sara Blecher part of a privileged community even during, apartheid. "On my grandfather's side we are Lithuanian Jews and he was the only one who escaped while the rest of the family was murdered during World War Ⅱ," she said. "Even though I am white, I am on the wrong side of history."
文摘BACKGROUND: Nattokinase (NK) is a serine protease enzyme of the subtilisin family. It exhibits a strong fibrinolytic activity. The fibrinolytic enzymes from Bacillus sp. have attracted interest as thrombolytic agents because of their efficiency in the fibrinolytic process including plasmin activation. METHODS: In the present study, VIT garden soil was collected and subjected to isolation process in order to screen for the NK production. Screening for NK enzyme was performed by radial caseinolytic assay. The production of NK enzyme was done in two different production medium for comparative studies. The NK enzyme was purified by gel permeation chromatography. The activity of the purified NK was checked by clot lysis and casein digestion assay. To investigate the structural basis of NK and fibrinogen interaction and also to identify the best binding mode, molecular dynamics and docking studies were performed. RESULTS: Based on the morphological and biochemical characterization, the isolate was identified as Bacillus sp. The overall purification fold of NK was about 3 with the specific activity of 664U/mg and 9.9% yield. Homogeneity of the purified enzyme was analyzed and confirmed by the single band obtained in SDS-PAGE. Molecular weight of the purified protease was estimated as 25 kDa. Purified NK enzyme exhibited 97% of effective clot lysis activity. The NK was docked in to the knob region of the fibrinogen at its binding site using Dock server. A total of 26 residues of fibrinogen and 29 residues of NK constitute the interface region. However, 9 residues offibrinogen (THR238, MET264, LYS266, ARG275, THR277, ALA279, ASN308, MET310, and LYS321) and 8 residues ofNK (GLY61, SER63, THR99, PHE189, LEU209, TYR217, ASN218, and MET222) are involved in intact binding. CONCLUSIONS: A significant amount of NK enzyme was obtained from Bacillus sp. The docking analysis revealed that the NK and fibrinogen adopt an extended binding pattern and interacts with the crucial residues to exhibit their activity.
文摘BACKGROUND: Screening of isolates for their potency to produce streptokinase was an important criterion of this research. The current study emphasizes the strain improvement, optimization and purification studies for enhanced production of streptokinase from Streptococcus uberis TNA-M1 isolated from bovine milk. METHODS: The study was carried out on samples collected from milk sample. Primary screening and characterization is used as an excellent source for the isolation of 13-hemolytic organisms. Strain improvement was done by both physical & chemical mutagenesis. The enzyme activity was checked by clot lysis assay and confirmed by fibrin plate method. The partially purified and crude enzyme were analysed by high-performance liquid chromatography. Molecular weight & enzyme purity was checked by SDS -PAGE, further confirmed by fibrin zymography. RESULTS: Out of the 3 isolated strains, only one isolate expressed 13-haemolysis with streptokinase (SK) activity. Based on the results of radial caseinolytic assay and blood clot dissolving assay, isolate TNA-M1 demonstrated the highest streptokinase activity. Based on morphological, biochemical and molecular characterization, it was identified as Streptococcus uberis and the strain was named as Streptococcus uberis TNA-M1. The results indicated that ultra-violet (UV) and ethyl methane sulfonate (EMS) were effective mutagenic agents for strain improvement of Streptococcus uberis TNA-M1 and enhanced SK productivity. HPLC analysis was performed in order to confirm the presence of streptokinase with the similar retention time (0.875 min) with its standard (0.854) min. SDS-PAGE of the enzyme showed protein band of approximately 47 kDa and confirmed by fibrin zymography. It exhibited fibrinolytic activity, which was more potent than other fibrinolytic enzymes. Glucose and peptone were recorded to be the optimum carbon and nitrogen sources respectively. CONCLUSION: Thus this study presents its novelty by highlighting the potential of Streptococcus uberis TNA-M1 as a significant source for the production of fibrinolytic enzymes.