OBJECTIVE: To study the molecular mechanism of Buyang Huanwu Tang( 补阳还五汤)(BHT) protecting retinal ganglion cells(RGCs) from oxygen induced oxidative stress and apoptosis after anterior ischemia. METHODS: In this ...OBJECTIVE: To study the molecular mechanism of Buyang Huanwu Tang( 补阳还五汤)(BHT) protecting retinal ganglion cells(RGCs) from oxygen induced oxidative stress and apoptosis after anterior ischemia. METHODS: In this study, the Chinese herbs of BHT were extracted by first boiling in water, then were filtered, concentrated, and freeze-dried. The chemical profile of BHT extract was determined by liquid chromatography mass spectrometry(LC-MS). H2O2-induced RGC-5 cells were used as a cell model to investigate the protective effect and mechanism of BHT on RGCs. RESULTS: The survival rate of damaged RGC-5 by BHT was significantly increased by the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazolium-romid method. Fluorescence activating cell sorter(FACS analysis) showed that BHT could significantly reduce apoptosis induced by oxidative stress via the reactive oxygen species(ROS)-mitogen-activated protein kinase(MAPK)-Caspase-3 signal pathway. CONCLUSION: BHT possesses a high antioxidant capacity and could significantly reduce ROS levels of RGC-5 cells damaged by H2O2.Therefore, the present study has provided possible alternative strategies for the prevention and treatment of ischemic optic disease by using traditional Chinese herbal formulas.展开更多
Objective:To explore the mechanism of Buyang Huanwu Tang (补阳还五汤 Decoction Invigorating Yang for Recuperation) combined with bone marrow mesenchymal stem cells (MSCs) transplantation in protecting nerves of cerebr...Objective:To explore the mechanism of Buyang Huanwu Tang (补阳还五汤 Decoction Invigorating Yang for Recuperation) combined with bone marrow mesenchymal stem cells (MSCs) transplantation in protecting nerves of cerebral ischemic injury. Methods: Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang(补阳还五汤)was administrated. Vascular endothelial growth factor (VEGF) and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results: VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group (P<0.05), and with the most strongest effect in the combination group. Conclusion: Buyang Huanwu Tang(补阳还五汤)combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.展开更多
目的:运用网络药理学方法建立补阳还五汤靶点-疾病网络,挖掘补阳还五汤治疗勃起功能障碍(erectile dysfunction,ED)的作用机制。方法:运用中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database ...目的:运用网络药理学方法建立补阳还五汤靶点-疾病网络,挖掘补阳还五汤治疗勃起功能障碍(erectile dysfunction,ED)的作用机制。方法:运用中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)收集补阳还五汤组成药物的主要活性成分,采用Swiss target prediction和TCMSP靶点预测工具预测药物成分潜在作用靶点,在GENECARDS等多个数据库获取ED相关靶标。寻找复方成分与疾病共有靶标,应用拓扑分析明确重要靶点,借助AVID在线工具进行关键作用靶点的基因本体论功能富集分析(gene ontology,GO)和KEGG富集(kyoto encyclopedia of genes and genomes,KEGG)分析。最后通过Cytoscape 3.5.1构建补阳还五汤中药-靶点-疾病可视化网络图。结果:补阳还五汤中共收集到符合条件的活性成分73个,其中黄芪18个,当归尾2个,赤芍14个,川芎6个,桃仁18个,红花15个。这些活性成分有621个潜在靶标,ED疾病靶标308个,补阳还五汤靶标基因与ED疾病靶标基因的交集基因有71个,其中hub节点36个。对这36个关键作用靶点进行整合分析发现,补阳还五汤主要通过细胞因子与细胞因子受体的相互作用、PI3K-Akt信号通路、肿瘤坏死因子信号通路、核转录因子κB信号通路、HIF-1信号通路、NOD样受体信号通路、Jak-STAT信号通路、p53信号通路、前列腺癌等途径实现对基因表达的干预,从而治疗ED。结论:网络药理学方法揭示补阳还五汤治疗ED是多成分、多靶点、多条信号通路共同作用的结果。展开更多
目的:探究地黄饮子加减与补阳还五汤治疗中风后失语症的临床疗效。方法:选取脑中风后失语症患者86例,按随机数字表法分为观察组与对照组,每组43例。两组均给予现代医学常规治疗,对照组给予补阳还五汤治疗,观察组给予补阳还五汤合地黄饮...目的:探究地黄饮子加减与补阳还五汤治疗中风后失语症的临床疗效。方法:选取脑中风后失语症患者86例,按随机数字表法分为观察组与对照组,每组43例。两组均给予现代医学常规治疗,对照组给予补阳还五汤治疗,观察组给予补阳还五汤合地黄饮子加减治疗。比较两组临床疗效、功能性语言沟通能力量表(Chinese functional communication profile,CFCP)评分、中国康复研究中心汉语标准失语症检查量表(the Chinese Rehabilitation Research Center Standard Aphasia Examination,CRRCAE)评分、美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、脑卒中失语患者生活质量量表(the Stroke and Aphasia Quality of Life Scale-39,SAQOL-39)评分、日常生活活动能力(Activities of Daily Living,ADL)评分及不良反应发生率。结果:观察组总有效率[88.37%(38/43)]高于对照组[65.12%(28/43)](P<0.01)。治疗前,两组CFCP各维度评分及总评分和CRRCAE、NIHSS、SAQOL-39及ADL评分比较,差异无统计学意义(P>0.05),治疗前后两组组内比较差异有统计学意义(P<0.05),治疗后两组组间比较,差异也有统计学意义(P<0.05)。观察组不良反应率[9.30%(4/43)]低于对照组[6.98%(3/43)],两组比较差异无统计学意义(P>0.05)。结论:在常规治疗的基础上,相较于服用补阳还五汤治疗,联合服用地黄饮子能更加明显地改善脑卒中失语症患者的神经功能、生活质量和日常生活活动能力,促进语言功能的恢复。展开更多
基金Supported by China Postdoctoral Science Foundation (Experimental Study on the Inhibitory Effect of Yiqi Tongluo Method on RGCs Apoptosis in AION Model Rats, No. 2017M621180)。
文摘OBJECTIVE: To study the molecular mechanism of Buyang Huanwu Tang( 补阳还五汤)(BHT) protecting retinal ganglion cells(RGCs) from oxygen induced oxidative stress and apoptosis after anterior ischemia. METHODS: In this study, the Chinese herbs of BHT were extracted by first boiling in water, then were filtered, concentrated, and freeze-dried. The chemical profile of BHT extract was determined by liquid chromatography mass spectrometry(LC-MS). H2O2-induced RGC-5 cells were used as a cell model to investigate the protective effect and mechanism of BHT on RGCs. RESULTS: The survival rate of damaged RGC-5 by BHT was significantly increased by the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazolium-romid method. Fluorescence activating cell sorter(FACS analysis) showed that BHT could significantly reduce apoptosis induced by oxidative stress via the reactive oxygen species(ROS)-mitogen-activated protein kinase(MAPK)-Caspase-3 signal pathway. CONCLUSION: BHT possesses a high antioxidant capacity and could significantly reduce ROS levels of RGC-5 cells damaged by H2O2.Therefore, the present study has provided possible alternative strategies for the prevention and treatment of ischemic optic disease by using traditional Chinese herbal formulas.
基金supported by Henan Province Higher Learning Institution Outstanding Scientific Research Talent Innovation Engineering Project (2007KYCX007)Henan Province Outstanding Youth Project (08100510015)
文摘Objective:To explore the mechanism of Buyang Huanwu Tang (补阳还五汤 Decoction Invigorating Yang for Recuperation) combined with bone marrow mesenchymal stem cells (MSCs) transplantation in protecting nerves of cerebral ischemic injury. Methods: Local cerebral ischemia-reperfusion rat model was established with modified Zea-Longa thread-occlusion method, and MSCs were injected into the caudal vein, and Buyang Huanwu Tang(补阳还五汤)was administrated. Vascular endothelial growth factor (VEGF) and Ki-67 expression in the ischemic side of the brain in the cerebral ischemic-reperfusion rat were detected with immuno-histochemical staining method. Results: VEGF and Ki-67 expressions were significantly up-regulated in the MSCs group and the combination group, with significant differences as compared with the model group and the sham operation group (P<0.05), and with the most strongest effect in the combination group. Conclusion: Buyang Huanwu Tang(补阳还五汤)combined with MSCs transplantation repairs the injured blood vessels and lesion tissues possibly by up-regulation of VEGF and Ki-67 expression.
文摘目的:运用网络药理学方法建立补阳还五汤靶点-疾病网络,挖掘补阳还五汤治疗勃起功能障碍(erectile dysfunction,ED)的作用机制。方法:运用中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)收集补阳还五汤组成药物的主要活性成分,采用Swiss target prediction和TCMSP靶点预测工具预测药物成分潜在作用靶点,在GENECARDS等多个数据库获取ED相关靶标。寻找复方成分与疾病共有靶标,应用拓扑分析明确重要靶点,借助AVID在线工具进行关键作用靶点的基因本体论功能富集分析(gene ontology,GO)和KEGG富集(kyoto encyclopedia of genes and genomes,KEGG)分析。最后通过Cytoscape 3.5.1构建补阳还五汤中药-靶点-疾病可视化网络图。结果:补阳还五汤中共收集到符合条件的活性成分73个,其中黄芪18个,当归尾2个,赤芍14个,川芎6个,桃仁18个,红花15个。这些活性成分有621个潜在靶标,ED疾病靶标308个,补阳还五汤靶标基因与ED疾病靶标基因的交集基因有71个,其中hub节点36个。对这36个关键作用靶点进行整合分析发现,补阳还五汤主要通过细胞因子与细胞因子受体的相互作用、PI3K-Akt信号通路、肿瘤坏死因子信号通路、核转录因子κB信号通路、HIF-1信号通路、NOD样受体信号通路、Jak-STAT信号通路、p53信号通路、前列腺癌等途径实现对基因表达的干预,从而治疗ED。结论:网络药理学方法揭示补阳还五汤治疗ED是多成分、多靶点、多条信号通路共同作用的结果。
文摘目的:探究地黄饮子加减与补阳还五汤治疗中风后失语症的临床疗效。方法:选取脑中风后失语症患者86例,按随机数字表法分为观察组与对照组,每组43例。两组均给予现代医学常规治疗,对照组给予补阳还五汤治疗,观察组给予补阳还五汤合地黄饮子加减治疗。比较两组临床疗效、功能性语言沟通能力量表(Chinese functional communication profile,CFCP)评分、中国康复研究中心汉语标准失语症检查量表(the Chinese Rehabilitation Research Center Standard Aphasia Examination,CRRCAE)评分、美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分、脑卒中失语患者生活质量量表(the Stroke and Aphasia Quality of Life Scale-39,SAQOL-39)评分、日常生活活动能力(Activities of Daily Living,ADL)评分及不良反应发生率。结果:观察组总有效率[88.37%(38/43)]高于对照组[65.12%(28/43)](P<0.01)。治疗前,两组CFCP各维度评分及总评分和CRRCAE、NIHSS、SAQOL-39及ADL评分比较,差异无统计学意义(P>0.05),治疗前后两组组内比较差异有统计学意义(P<0.05),治疗后两组组间比较,差异也有统计学意义(P<0.05)。观察组不良反应率[9.30%(4/43)]低于对照组[6.98%(3/43)],两组比较差异无统计学意义(P>0.05)。结论:在常规治疗的基础上,相较于服用补阳还五汤治疗,联合服用地黄饮子能更加明显地改善脑卒中失语症患者的神经功能、生活质量和日常生活活动能力,促进语言功能的恢复。
