Determination of rhynchophylline,isorhynchophylline,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San by HPLC

OBJECTIVE: To develop an HPLC method for the simultaneous determination of rhynchophylline, isorhynchophylline,prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.METHODS: The chromatograhic separation was performed on C_(18) column(4.6 mm×200 mm, 5 μm), a linear elution of acetonitrile-methanol(1:4) and 0.1% triethylamine solution was adopted at the flow rate of 0.7 mL·min^(-1). The detection wavelength was 254 nm and the column temperature was 30 ℃.RESULTS: Rhynchophylline, isorhynchophylline, prim-Oglucosylcimifugin and 5-O-methylvisammioside had a good linearity at 5.10 to 102.00 μg·mL^(-1)(r=0.9994), 5.95 to 119.00μg·mL^(-1)(r=0.9996), 6.10 to 122.00 μg·mL^(-1)(r=0.9999), 5.65 to113.00 μg·mL^(-1)(r=0.9998), respectively. The average recoveries and RSD were 97.66%(1.32%), 96.72%(0.52%), 98.99%(0.78%) and 99.16%(1.56%), respectively.CONCLUSION: The method was simple, convenience and may be used in the determination of rhynchophylline, isorhynchophylline, prim-O-glucosylcimifugin and 5-O-methylvisammioside in Babaojingfeng San.

UHPLC法测定八宝惊风散中沉香四醇的含量

目的建立超高效液相色谱(UHPLC)法测定八宝惊风散中沉香四醇含量的方法。方法采用Agilent Poroshell 120 SBC18(100 mm×4.6 mm,2.7μm)为色谱柱固定相,以乙腈(A)-0.1%甲酸水溶液(B)为流动相,梯度洗脱,流速为0.8 mL·min^(-1);柱温为40℃;检测波长为252 nm。结果沉香四醇实现较好地分离,在4.02~40.2μg·mL^(-1)范围内线性良好(r=0.999 9);平均回收率为98.90%(n=6),RSD为1.64%。结论该方法测定结果准确可靠,重现性好,可以作为八宝惊风散质量控制的参考依据。